Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia
With advances in sequencing technologies, there has been an increase in the discovery of viruses that do not group with any currently described virus families. The newly described taxon Negevirus encompasses a group of viruses displaying an insect-specific phenotype which have been isolated from mul...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-02-01
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| Series: | Evolutionary Bioinformatics |
| Online Access: | https://doi.org/10.1177/1176934317691269 |
| _version_ | 1811330657819820032 |
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| author | Caitlin A O’Brien Breeanna J McLean Agathe M G Colmant Jessica J Harrison Sonja Hall-Mendelin Andrew F van den Hurk Cheryl A Johansen Daniel Watterson Helle Bielefeldt-Ohmann Natalee D Newton Benjamin L Schulz Roy A Hall Jody Hobson-Peters |
| author_facet | Caitlin A O’Brien Breeanna J McLean Agathe M G Colmant Jessica J Harrison Sonja Hall-Mendelin Andrew F van den Hurk Cheryl A Johansen Daniel Watterson Helle Bielefeldt-Ohmann Natalee D Newton Benjamin L Schulz Roy A Hall Jody Hobson-Peters |
| author_sort | Caitlin A O’Brien |
| collection | DOAJ |
| description | With advances in sequencing technologies, there has been an increase in the discovery of viruses that do not group with any currently described virus families. The newly described taxon Negevirus encompasses a group of viruses displaying an insect-specific phenotype which have been isolated from multiple host species on numerous continents. Using a broad-spectrum virus screening assay based on the detection of double-stranded RNA and next-generation sequencing, we have detected a novel species of negevirus, from Anopheles, Culex , and Aedes mosquitoes collected in 4 geographically separate regions of Australia. Bioinformatic analysis of the virus, tentatively named Castlerea virus, revealed that it is genetically distinct from previously described negeviruses but clusters in the newly proposed Nelorpivirus clade within this taxon. Analysis of virions confirmed the presence of 2 proteins of 24 and 40 kDa which support previous bioinformatic predictions of negevirus structural proteins. |
| first_indexed | 2024-04-13T16:06:58Z |
| format | Article |
| id | doaj.art-92a28ebc71c84f91858fd23bba2e35ff |
| institution | Directory Open Access Journal |
| issn | 1176-9343 |
| language | English |
| last_indexed | 2024-04-13T16:06:58Z |
| publishDate | 2017-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Evolutionary Bioinformatics |
| spelling | doaj.art-92a28ebc71c84f91858fd23bba2e35ff2022-12-22T02:40:22ZengSAGE PublishingEvolutionary Bioinformatics1176-93432017-02-011310.1177/117693431769126910.1177_1176934317691269Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in AustraliaCaitlin A O’Brien0Breeanna J McLean1Agathe M G Colmant2Jessica J Harrison3Sonja Hall-Mendelin4Andrew F van den Hurk5Cheryl A Johansen6Daniel Watterson7Helle Bielefeldt-Ohmann8Natalee D Newton9Benjamin L Schulz10Roy A Hall11Jody Hobson-Peters12Australian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaPublic Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Coopers Plains, QLD, AustraliaPublic Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Coopers Plains, QLD, AustraliaDepartment of Health – Pathwest Laboratory Medicine WA, Nedlands, WA, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaWith advances in sequencing technologies, there has been an increase in the discovery of viruses that do not group with any currently described virus families. The newly described taxon Negevirus encompasses a group of viruses displaying an insect-specific phenotype which have been isolated from multiple host species on numerous continents. Using a broad-spectrum virus screening assay based on the detection of double-stranded RNA and next-generation sequencing, we have detected a novel species of negevirus, from Anopheles, Culex , and Aedes mosquitoes collected in 4 geographically separate regions of Australia. Bioinformatic analysis of the virus, tentatively named Castlerea virus, revealed that it is genetically distinct from previously described negeviruses but clusters in the newly proposed Nelorpivirus clade within this taxon. Analysis of virions confirmed the presence of 2 proteins of 24 and 40 kDa which support previous bioinformatic predictions of negevirus structural proteins.https://doi.org/10.1177/1176934317691269 |
| spellingShingle | Caitlin A O’Brien Breeanna J McLean Agathe M G Colmant Jessica J Harrison Sonja Hall-Mendelin Andrew F van den Hurk Cheryl A Johansen Daniel Watterson Helle Bielefeldt-Ohmann Natalee D Newton Benjamin L Schulz Roy A Hall Jody Hobson-Peters Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia Evolutionary Bioinformatics |
| title | Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia |
| title_full | Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia |
| title_fullStr | Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia |
| title_full_unstemmed | Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia |
| title_short | Discovery and Characterisation of Castlerea Virus, a New Species of Isolated in Australia |
| title_sort | discovery and characterisation of castlerea virus a new species of isolated in australia |
| url | https://doi.org/10.1177/1176934317691269 |
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