Recommendations for good practice in MS-based lipidomics
In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227521001206 |
| _version_ | 1819320408116035584 |
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| author | Harald C. Köfeler Robert Ahrends Erin S. Baker Kim Ekroos Xianlin Han Nils Hoffmann Michal Holčapek Markus R. Wenk Gerhard Liebisch |
| author_facet | Harald C. Köfeler Robert Ahrends Erin S. Baker Kim Ekroos Xianlin Han Nils Hoffmann Michal Holčapek Markus R. Wenk Gerhard Liebisch |
| author_sort | Harald C. Köfeler |
| collection | DOAJ |
| description | In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field. |
| first_indexed | 2024-12-24T11:19:06Z |
| format | Article |
| id | doaj.art-92a585f7f3db439c898bcd07c0242453 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-24T11:19:06Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-92a585f7f3db439c898bcd07c02424532022-12-21T16:58:18ZengElsevierJournal of Lipid Research0022-22752021-01-0162100138Recommendations for good practice in MS-based lipidomicsHarald C. Köfeler0Robert Ahrends1Erin S. Baker2Kim Ekroos3Xianlin Han4Nils Hoffmann5Michal Holčapek6Markus R. Wenk7Gerhard Liebisch8Core Facility Mass Spectrometry, Medical University of Graz, Graz, Austria; For correspondence: Harald C. Köfeler; Gerhard LiebischDepartment for Analytical Chemistry, University of Vienna, Vienna, AustriaDepartment of Chemistry, North Carolina State University, Raleigh, NC, USALipidomics Consulting Ltd., Esbo, FinlandBarshop Inst Longev & Aging Studies, Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX, USACenter for Biotechnology, Universität Bielefeld, Bielefeld, GermanyDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech RepublicSingapore Lipidomics Incubator (SLING), Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore, SingaporeInstitute of Clinical Chemistry and Laboratory Medicine, Regensburg University Hospital, Regensburg, Germany; For correspondence: Harald C. Köfeler; Gerhard LiebischIn the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.http://www.sciencedirect.com/science/article/pii/S0022227521001206lipidomicsmetabolomicsMSchromatographyion mobility spectrometryphospholipids |
| spellingShingle | Harald C. Köfeler Robert Ahrends Erin S. Baker Kim Ekroos Xianlin Han Nils Hoffmann Michal Holčapek Markus R. Wenk Gerhard Liebisch Recommendations for good practice in MS-based lipidomics Journal of Lipid Research lipidomics metabolomics MS chromatography ion mobility spectrometry phospholipids |
| title | Recommendations for good practice in MS-based lipidomics |
| title_full | Recommendations for good practice in MS-based lipidomics |
| title_fullStr | Recommendations for good practice in MS-based lipidomics |
| title_full_unstemmed | Recommendations for good practice in MS-based lipidomics |
| title_short | Recommendations for good practice in MS-based lipidomics |
| title_sort | recommendations for good practice in ms based lipidomics |
| topic | lipidomics metabolomics MS chromatography ion mobility spectrometry phospholipids |
| url | http://www.sciencedirect.com/science/article/pii/S0022227521001206 |
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