Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits
Introduction: In our country RT-PCR is the only method used to diagnose COVID-19 infection, caused by SARS-CoV-2, one of the greatest epidemic in world history. In this study we aimed to compare two most frequently used commercial diagnostic kits. Materials and Methods: A total of 100 samples wh...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
2022-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-01/177-182%20Sebnem%20Senol%20Akar%202.pdf |
| Summary: | Introduction: In our country RT-PCR is the only method used to diagnose COVID-19 infection, caused by SARS-CoV-2, one of the greatest epidemic in world history. In this study we aimed to compare two most frequently used commercial diagnostic kits.
Materials and Methods: A total of 100 samples which were referred to our laboratory were used in this study. These nucleic acid samples were diagnosed as positive (50) or negative (50) by Coronex® COVID-19 (Ver.2.0) Multipleks RT-qPCR Diagnosis Kit (DS Bio and Nano Technology, Ankara, Turkey) and kept at -20°C. The samples were cross checked with RealStar® SARS-CoV-2 RT-PCR Kit 1.0 (Altona Diagnostic, Hamburg, Germany). Extraction of the samples was performed by QIAsymphony (Qiagen, Hollanda). Data was evaluated with kappa analysis and t test.
Results: All of the 50 positive samples were positive with Real Star® as well. Two of the negative samples were found positive when studied with Real Star®. There was a high concordance between the two kits (Kappa= 0.96). Mean Ct values were found as 24.1 ± 4.9 and 19.6 ± 4.2 for Real Star® and Coronex®, respectively. The Ct value was found less than 20 in 51.9% of the 52 positive samples studied with Real Star®.
Conclusion: There is a high concordance between the two commercial kits. Both kits may be used with confidence in symptomatic patients for the diagnosis of COVID-19. |
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| ISSN: | 1300-932X 1308-5115 |