Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits
Introduction: In our country RT-PCR is the only method used to diagnose COVID-19 infection, caused by SARS-CoV-2, one of the greatest epidemic in world history. In this study we aimed to compare two most frequently used commercial diagnostic kits. Materials and Methods: A total of 100 samples wh...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-01/177-182%20Sebnem%20Senol%20Akar%202.pdf |
| _version_ | 1797923489050525696 |
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| author | Şebnem ŞENOL AKAR Sinem AKÇALI |
| author_facet | Şebnem ŞENOL AKAR Sinem AKÇALI |
| author_sort | Şebnem ŞENOL AKAR |
| collection | DOAJ |
| description | Introduction: In our country RT-PCR is the only method used to diagnose COVID-19 infection, caused by SARS-CoV-2, one of the greatest epidemic in world history. In this study we aimed to compare two most frequently used commercial diagnostic kits.
Materials and Methods: A total of 100 samples which were referred to our laboratory were used in this study. These nucleic acid samples were diagnosed as positive (50) or negative (50) by Coronex® COVID-19 (Ver.2.0) Multipleks RT-qPCR Diagnosis Kit (DS Bio and Nano Technology, Ankara, Turkey) and kept at -20°C. The samples were cross checked with RealStar® SARS-CoV-2 RT-PCR Kit 1.0 (Altona Diagnostic, Hamburg, Germany). Extraction of the samples was performed by QIAsymphony (Qiagen, Hollanda). Data was evaluated with kappa analysis and t test.
Results: All of the 50 positive samples were positive with Real Star® as well. Two of the negative samples were found positive when studied with Real Star®. There was a high concordance between the two kits (Kappa= 0.96). Mean Ct values were found as 24.1 ± 4.9 and 19.6 ± 4.2 for Real Star® and Coronex®, respectively. The Ct value was found less than 20 in 51.9% of the 52 positive samples studied with Real Star®.
Conclusion: There is a high concordance between the two commercial kits. Both kits may be used with confidence in symptomatic patients for the diagnosis of COVID-19. |
| first_indexed | 2024-04-10T14:47:00Z |
| format | Article |
| id | doaj.art-92a707c1de514fd3ac207563bc28205f |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T14:47:00Z |
| publishDate | 2022-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-92a707c1de514fd3ac207563bc28205f2023-02-15T16:07:50ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-03-0127117718210.5578/flora.20229913Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic KitsŞebnem ŞENOL AKAR0https://orcid.org/0000-0001-7438-7306Sinem AKÇALI1Department of Infectious Diseases and Clinical Microbiology, Manisa Celal Bayar University Faculty of Medicine, Manisa, TurkeyDepartment of Medical Microbiology, Manisa Celal Bayar University Faculty of Medicine, Manisa, TurkeyIntroduction: In our country RT-PCR is the only method used to diagnose COVID-19 infection, caused by SARS-CoV-2, one of the greatest epidemic in world history. In this study we aimed to compare two most frequently used commercial diagnostic kits. Materials and Methods: A total of 100 samples which were referred to our laboratory were used in this study. These nucleic acid samples were diagnosed as positive (50) or negative (50) by Coronex® COVID-19 (Ver.2.0) Multipleks RT-qPCR Diagnosis Kit (DS Bio and Nano Technology, Ankara, Turkey) and kept at -20°C. The samples were cross checked with RealStar® SARS-CoV-2 RT-PCR Kit 1.0 (Altona Diagnostic, Hamburg, Germany). Extraction of the samples was performed by QIAsymphony (Qiagen, Hollanda). Data was evaluated with kappa analysis and t test. Results: All of the 50 positive samples were positive with Real Star® as well. Two of the negative samples were found positive when studied with Real Star®. There was a high concordance between the two kits (Kappa= 0.96). Mean Ct values were found as 24.1 ± 4.9 and 19.6 ± 4.2 for Real Star® and Coronex®, respectively. The Ct value was found less than 20 in 51.9% of the 52 positive samples studied with Real Star®. Conclusion: There is a high concordance between the two commercial kits. Both kits may be used with confidence in symptomatic patients for the diagnosis of COVID-19.http://floradergisi.org/managete/fu_folder/2022-01/177-182%20Sebnem%20Senol%20Akar%202.pdfsars-cov-2rt-pcrin vitro diagnostics |
| spellingShingle | Şebnem ŞENOL AKAR Sinem AKÇALI Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi sars-cov-2 rt-pcr in vitro diagnostics |
| title | Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits |
| title_full | Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits |
| title_fullStr | Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits |
| title_full_unstemmed | Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits |
| title_short | Comparison of Two Different Commercial SARS-CoV-2 PCR Diagnostic Kits |
| title_sort | comparison of two different commercial sars cov 2 pcr diagnostic kits |
| topic | sars-cov-2 rt-pcr in vitro diagnostics |
| url | http://floradergisi.org/managete/fu_folder/2022-01/177-182%20Sebnem%20Senol%20Akar%202.pdf |
| work_keys_str_mv | AT sebnemsenolakar comparisonoftwodifferentcommercialsarscov2pcrdiagnostickits AT sinemakcali comparisonoftwodifferentcommercialsarscov2pcrdiagnostickits |