Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii
The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. ro...
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X2200003X |
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| author | Xue Liu Lingxia Zhou Biyun Luo Hongli Qian Baoqing Ye Keyi Ma Gaofeng Qiu |
| author_facet | Xue Liu Lingxia Zhou Biyun Luo Hongli Qian Baoqing Ye Keyi Ma Gaofeng Qiu |
| author_sort | Xue Liu |
| collection | DOAJ |
| description | The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M. rosenbergii. |
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| issn | 2468-550X |
| language | English |
| last_indexed | 2025-03-22T04:41:39Z |
| publishDate | 2023-09-01 |
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| series | Aquaculture and Fisheries |
| spelling | doaj.art-92a910bdef794269aed8ac931da633b82024-04-27T22:27:10ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2023-09-0185509513Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergiiXue Liu0Lingxia Zhou1Biyun Luo2Hongli Qian3Baoqing Ye4Keyi Ma5Gaofeng Qiu6National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, ChinaTemasek Life Sciences Laboratory, National University of Singapore, 117604, Republic of SingaporeNational Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, China; Corresponding author. College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Pudong New Area, Shanghai, 201306, China.National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), Shanghai Engineering Research Center of Aquaculture (Shanghai Ocean University), Shanghai, 201306, China; Corresponding author. College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Pudong New Area, Shanghai, 201306, China.The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M. rosenbergii.http://www.sciencedirect.com/science/article/pii/S2468550X2200003XSex chromosome-specific markerZW/ZZMacrobrachium rosenbergii |
| spellingShingle | Xue Liu Lingxia Zhou Biyun Luo Hongli Qian Baoqing Ye Keyi Ma Gaofeng Qiu Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii Aquaculture and Fisheries Sex chromosome-specific marker ZW/ZZ Macrobrachium rosenbergii |
| title | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii |
| title_full | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii |
| title_fullStr | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii |
| title_full_unstemmed | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii |
| title_short | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii |
| title_sort | identification of novel z w chromosome specific markers from the giant freshwater prawn macrobrachium rosenbergii |
| topic | Sex chromosome-specific marker ZW/ZZ Macrobrachium rosenbergii |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X2200003X |
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