Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model
Glioblastoma multiforme (GBM) is a malignant tumor with a higher prevalence in men and a higher survival rate in transmenopausal women. It exhibits distinct areas influenced by changing environmental conditions. This study examines how these areas differ in the levels of estrogen receptors (ERs) whi...
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MDPI AG
2024-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Donata Simińska Klaudyna Kojder Dariusz Jeżewski Maciej Tarnowski Patrycja Tomasiak Katarzyna Piotrowska Agnieszka Kolasa Kapczuk Patrycja Dariusz Chlubek Irena Baranowska-Bosiacka |
| author_facet | Donata Simińska Klaudyna Kojder Dariusz Jeżewski Maciej Tarnowski Patrycja Tomasiak Katarzyna Piotrowska Agnieszka Kolasa Kapczuk Patrycja Dariusz Chlubek Irena Baranowska-Bosiacka |
| author_sort | Donata Simińska |
| collection | DOAJ |
| description | Glioblastoma multiforme (GBM) is a malignant tumor with a higher prevalence in men and a higher survival rate in transmenopausal women. It exhibits distinct areas influenced by changing environmental conditions. This study examines how these areas differ in the levels of estrogen receptors (ERs) which play an important role in the development and progression of many cancers, and whose expression levels are often correlated with patient survival. This study utilized two research models: an in vitro model employing the U87 cell line and a second model involving tumors resected from patients (including tumor core, enhancing tumor region, and peritumoral area). ER expression was assessed at both gene and protein levels, with the results validated using confocal microscopy and immunohistochemistry. Under hypoxic conditions, the U87 line displayed a decrease in ERβ mRNA expression and an increase in ERα mRNA expression. In patient samples, ERβ mRNA expression was lower in the tumor core compared to the enhancing tumor region (only in males when the study group was divided by sex). In addition, ERβ protein expression was lower in the tumor core than in the peritumoral area (only in women when the study group was divided by sex). Immunohistochemical analysis indicated the highest ERβ protein expression in the enhancing tumor area, followed by the peritumoral area, and the lowest in the tumor core. The findings suggest that ER expression may significantly influence the development of GBM, exhibiting variability under the influence of conditions present in different tumor areas. |
| first_indexed | 2024-04-24T10:42:27Z |
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| language | English |
| last_indexed | 2024-04-24T10:42:27Z |
| publishDate | 2024-04-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-92af3a9b62db4c4aaedd5d30d034c2112024-04-12T13:20:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257413010.3390/ijms25074130Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro ModelDonata Simińska0Klaudyna Kojder1Dariusz Jeżewski2Maciej Tarnowski3Patrycja Tomasiak4Katarzyna Piotrowska5Agnieszka Kolasa6Kapczuk Patrycja7Dariusz Chlubek8Irena Baranowska-Bosiacka9Department of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Anaesthesiology and Intensive Care, Pomeranian Medical University in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, PolandDepartment of Neurosurgery and Pediatric Neurosurgery, Pomeranian Medical University in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, PolandDepartment of Physiology in Health Sciences, Pomeranian Medical University in Szczecin, Żołnierska 54, 70-210 Szczecin, PolandInstitute of Physical Culture Sciences, University of Szczecin, 70-453 Szczecin, PolandDepartment of Physiology, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Histology and Embryology, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, PolandGlioblastoma multiforme (GBM) is a malignant tumor with a higher prevalence in men and a higher survival rate in transmenopausal women. It exhibits distinct areas influenced by changing environmental conditions. This study examines how these areas differ in the levels of estrogen receptors (ERs) which play an important role in the development and progression of many cancers, and whose expression levels are often correlated with patient survival. This study utilized two research models: an in vitro model employing the U87 cell line and a second model involving tumors resected from patients (including tumor core, enhancing tumor region, and peritumoral area). ER expression was assessed at both gene and protein levels, with the results validated using confocal microscopy and immunohistochemistry. Under hypoxic conditions, the U87 line displayed a decrease in ERβ mRNA expression and an increase in ERα mRNA expression. In patient samples, ERβ mRNA expression was lower in the tumor core compared to the enhancing tumor region (only in males when the study group was divided by sex). In addition, ERβ protein expression was lower in the tumor core than in the peritumoral area (only in women when the study group was divided by sex). Immunohistochemical analysis indicated the highest ERβ protein expression in the enhancing tumor area, followed by the peritumoral area, and the lowest in the tumor core. The findings suggest that ER expression may significantly influence the development of GBM, exhibiting variability under the influence of conditions present in different tumor areas.https://www.mdpi.com/1422-0067/25/7/4130estrogen receptor αestrogen receptor βglioblastoma multiformehypoxianutrient deficiencynuclear sex hormone receptors |
| spellingShingle | Donata Simińska Klaudyna Kojder Dariusz Jeżewski Maciej Tarnowski Patrycja Tomasiak Katarzyna Piotrowska Agnieszka Kolasa Kapczuk Patrycja Dariusz Chlubek Irena Baranowska-Bosiacka Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model International Journal of Molecular Sciences estrogen receptor α estrogen receptor β glioblastoma multiforme hypoxia nutrient deficiency nuclear sex hormone receptors |
| title | Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model |
| title_full | Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model |
| title_fullStr | Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model |
| title_full_unstemmed | Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model |
| title_short | Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model |
| title_sort | estrogen α and β receptor expression in the various regions of resected glioblastoma multiforme tumors and in an in vitro model |
| topic | estrogen receptor α estrogen receptor β glioblastoma multiforme hypoxia nutrient deficiency nuclear sex hormone receptors |
| url | https://www.mdpi.com/1422-0067/25/7/4130 |
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