A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells
Innate lymphoid cells (ILCs) play important roles in tissue homeostasis and host defense, but the proliferative properties and migratory behavior of especially human ILCs remain poorly understood. Here we mapped at single-cell resolution the spatial distribution of quiescent and proliferative human...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.902881/full |
| _version_ | 1811312713966551040 |
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| author | Yu Gao Arlisa Alisjahbana Daryl Zhong Hao Boey Imran Mohammad Natalie Sleiers Joakim S. Dahlin Tim Willinger |
| author_facet | Yu Gao Arlisa Alisjahbana Daryl Zhong Hao Boey Imran Mohammad Natalie Sleiers Joakim S. Dahlin Tim Willinger |
| author_sort | Yu Gao |
| collection | DOAJ |
| description | Innate lymphoid cells (ILCs) play important roles in tissue homeostasis and host defense, but the proliferative properties and migratory behavior of especially human ILCs remain poorly understood. Here we mapped at single-cell resolution the spatial distribution of quiescent and proliferative human ILCs within the vascular versus tissue compartment. For this purpose, we employed MISTRG humanized mice as an in-vivo model to study human ILCs. We uncovered subset-specific differences in the proliferative status between vascular and tissue ILCs within lymphoid and non-lymphoid organs. We also identified CD117-CRTH2-CD45RA+ ILCs in the spleen that were highly proliferative and expressed the transcription factor TCF-1. These proliferative ILCs were present during the neonatal period in human blood and emerged early during population of the human ILC compartment in MISTRG mice transplanted with human hematopoietic stem and progenitor cells (HSPCs). Single-cell RNA-sequencing combined with intravascular cell labeling suggested that proliferative ILCs actively migrated from the local vasculature into the spleen tissue. Collectively, our comprehensive map reveals the proliferative topography of human ILCs, linking cell migration and spatial compartmentalization with cell division. |
| first_indexed | 2024-04-13T10:41:30Z |
| format | Article |
| id | doaj.art-92b31eda76d3420e9350de4cd89f11b5 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T10:41:30Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-92b31eda76d3420e9350de4cd89f11b52022-12-22T02:49:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.902881902881A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cellsYu Gao0Arlisa Alisjahbana1Daryl Zhong Hao Boey2Imran Mohammad3Natalie Sleiers4Joakim S. Dahlin5Tim Willinger6Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenDepartment of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenDepartment of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenInnate lymphoid cells (ILCs) play important roles in tissue homeostasis and host defense, but the proliferative properties and migratory behavior of especially human ILCs remain poorly understood. Here we mapped at single-cell resolution the spatial distribution of quiescent and proliferative human ILCs within the vascular versus tissue compartment. For this purpose, we employed MISTRG humanized mice as an in-vivo model to study human ILCs. We uncovered subset-specific differences in the proliferative status between vascular and tissue ILCs within lymphoid and non-lymphoid organs. We also identified CD117-CRTH2-CD45RA+ ILCs in the spleen that were highly proliferative and expressed the transcription factor TCF-1. These proliferative ILCs were present during the neonatal period in human blood and emerged early during population of the human ILC compartment in MISTRG mice transplanted with human hematopoietic stem and progenitor cells (HSPCs). Single-cell RNA-sequencing combined with intravascular cell labeling suggested that proliferative ILCs actively migrated from the local vasculature into the spleen tissue. Collectively, our comprehensive map reveals the proliferative topography of human ILCs, linking cell migration and spatial compartmentalization with cell division.https://www.frontiersin.org/articles/10.3389/fimmu.2022.902881/fullinnate lymphoid cellsproliferationmigrationtissue residencyontogenyspleen |
| spellingShingle | Yu Gao Arlisa Alisjahbana Daryl Zhong Hao Boey Imran Mohammad Natalie Sleiers Joakim S. Dahlin Tim Willinger A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells Frontiers in Immunology innate lymphoid cells proliferation migration tissue residency ontogeny spleen |
| title | A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells |
| title_full | A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells |
| title_fullStr | A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells |
| title_full_unstemmed | A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells |
| title_short | A single-cell map of vascular and tissue lymphocytes identifies proliferative TCF-1+ human innate lymphoid cells |
| title_sort | single cell map of vascular and tissue lymphocytes identifies proliferative tcf 1 human innate lymphoid cells |
| topic | innate lymphoid cells proliferation migration tissue residency ontogeny spleen |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.902881/full |
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