Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology
<em>Capparis</em> <em>spionosa</em> L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of &...
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MDPI AG
2012-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/6/7323 |
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| author | Ling-Wen Zhang Zhen-Feng Liu Li-Na Jin Dong-Bin Ma Jing Miao Yu-Bin Ji Fang Dong |
| author_facet | Ling-Wen Zhang Zhen-Feng Liu Li-Na Jin Dong-Bin Ma Jing Miao Yu-Bin Ji Fang Dong |
| author_sort | Ling-Wen Zhang |
| collection | DOAJ |
| description | <em>Capparis</em> <em>spionosa</em> L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of <em>Capparis</em> <em>spionosa</em> L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the <em>in vivo</em> anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%, <em>p </em>> 0.05). This indicated that the model was adequate for the extraction process. Additionally, CSPS could prolong the survival time of H<sub>22</sub> bearing mice <em>in vivo</em>. The anti-tumor activities of CSPS were dose-dependent. |
| first_indexed | 2024-12-22T02:13:36Z |
| format | Article |
| id | doaj.art-92b479634909414f89afab4287d7d416 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T02:13:36Z |
| publishDate | 2012-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-92b479634909414f89afab4287d7d4162022-12-21T18:42:20ZengMDPI AGMolecules1420-30492012-06-011767323733510.3390/molecules17067323Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface MethodologyLing-Wen ZhangZhen-Feng LiuLi-Na JinDong-Bin MaJing MiaoYu-Bin JiFang Dong<em>Capparis</em> <em>spionosa</em> L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of <em>Capparis</em> <em>spionosa</em> L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the <em>in vivo</em> anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%, <em>p </em>> 0.05). This indicated that the model was adequate for the extraction process. Additionally, CSPS could prolong the survival time of H<sub>22</sub> bearing mice <em>in vivo</em>. The anti-tumor activities of CSPS were dose-dependent.http://www.mdpi.com/1420-3049/17/6/7323polysaccharides of <em>Capparis</em> <em>spionosa</em> L. (CSPS)extraction optimizationresponse surface methodologyanti-tumor activity |
| spellingShingle | Ling-Wen Zhang Zhen-Feng Liu Li-Na Jin Dong-Bin Ma Jing Miao Yu-Bin Ji Fang Dong Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology Molecules polysaccharides of <em>Capparis</em> <em>spionosa</em> L. (CSPS) extraction optimization response surface methodology anti-tumor activity |
| title | Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology |
| title_full | Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology |
| title_fullStr | Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology |
| title_full_unstemmed | Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology |
| title_short | Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of <em>Capparis</em> <em>spionosa</em> L. by Response Surface Methodology |
| title_sort | optimizing the extraction of anti tumor polysaccharides from the fruit of lt em gt capparis lt em gt lt em gt spionosa lt em gt l by response surface methodology |
| topic | polysaccharides of <em>Capparis</em> <em>spionosa</em> L. (CSPS) extraction optimization response surface methodology anti-tumor activity |
| url | http://www.mdpi.com/1420-3049/17/6/7323 |
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