Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma
NFL-TBS.40-63 peptide is a recently discovered peptide derived from the light neurofilament chain (NFL). In this study, we demonstrated that the Biotinylated-NFL-peptide (BIOT-NFL) can spontaneously self-assemble into well-organized nanofibers (approximately 5 nm width and several micrometers in len...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | International Journal of Pharmaceutics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590156722000172 |
| _version_ | 1818018120179122176 |
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| author | H. Alnemeh-Al Ali A. Griveau F. Artzner A. Dupont N. Lautram M.A. Jourdain J. Eyer |
| author_facet | H. Alnemeh-Al Ali A. Griveau F. Artzner A. Dupont N. Lautram M.A. Jourdain J. Eyer |
| author_sort | H. Alnemeh-Al Ali |
| collection | DOAJ |
| description | NFL-TBS.40-63 peptide is a recently discovered peptide derived from the light neurofilament chain (NFL). In this study, we demonstrated that the Biotinylated-NFL-peptide (BIOT-NFL) can spontaneously self-assemble into well-organized nanofibers (approximately 5 nm width and several micrometers in length) in several solutions, whereas the typical self-assembly was not systematically observed from other peptides with or without coupling. The critical aggregation concentration that allows the BIOT-NFL-peptide to aggregate and auto associate was determined at 10−4 mol/L by surface tension measurements. X-ray scattering of BIOT-NFL-peptide also demonstrated its beta-sheet structure that can facilitate the intermolecular interactions involved in the self-assembly process. The possible disassembly of self-assembled BIOT-NFL-peptide-nanofibers was examined via a dialysis membrane study. We further investigated the interaction between nanofibers formed by BIOT-NFL-peptide and gold nanoparticles. Interestingly, a strong interaction was demonstrated between these nanoparticles and BIOT-NFL-peptide resulted in the formation of BIOT-NFL-peptide-nanofibers grandly decorated by gold nanoparticles. Finally, we investigated the internalization of gold nanoparticles coupled with BIOT-NFL-nanofibers into F98 rat glioblastoma cells, which was increased compared to the non-coupled control gold nanoparticles. All these results indicate that this peptide could be a promising therapeutic agent for targeted delivery. |
| first_indexed | 2024-04-14T07:35:00Z |
| format | Article |
| id | doaj.art-92b57a9f93f54f549ae3177066762e34 |
| institution | Directory Open Access Journal |
| issn | 2590-1567 |
| language | English |
| last_indexed | 2024-04-14T07:35:00Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Pharmaceutics: X |
| spelling | doaj.art-92b57a9f93f54f549ae3177066762e342022-12-22T02:05:42ZengElsevierInternational Journal of Pharmaceutics: X2590-15672022-12-014100128Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastomaH. Alnemeh-Al Ali0A. Griveau1F. Artzner2A. Dupont3N. Lautram4M.A. Jourdain5J. Eyer6Univ Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, FranceUniv Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, FranceUniv Rennes, CNRS, IPR (Institut de Physique de Rennes), UMR 6251, F-35000 Rennes, FranceUniv Rennes, CNRS, Inserm, BIOSIT-UMS 3480, US_S 018, Rennes, FranceUniv Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, FranceUniv Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, FranceUniv Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, France; Corresponding author.NFL-TBS.40-63 peptide is a recently discovered peptide derived from the light neurofilament chain (NFL). In this study, we demonstrated that the Biotinylated-NFL-peptide (BIOT-NFL) can spontaneously self-assemble into well-organized nanofibers (approximately 5 nm width and several micrometers in length) in several solutions, whereas the typical self-assembly was not systematically observed from other peptides with or without coupling. The critical aggregation concentration that allows the BIOT-NFL-peptide to aggregate and auto associate was determined at 10−4 mol/L by surface tension measurements. X-ray scattering of BIOT-NFL-peptide also demonstrated its beta-sheet structure that can facilitate the intermolecular interactions involved in the self-assembly process. The possible disassembly of self-assembled BIOT-NFL-peptide-nanofibers was examined via a dialysis membrane study. We further investigated the interaction between nanofibers formed by BIOT-NFL-peptide and gold nanoparticles. Interestingly, a strong interaction was demonstrated between these nanoparticles and BIOT-NFL-peptide resulted in the formation of BIOT-NFL-peptide-nanofibers grandly decorated by gold nanoparticles. Finally, we investigated the internalization of gold nanoparticles coupled with BIOT-NFL-nanofibers into F98 rat glioblastoma cells, which was increased compared to the non-coupled control gold nanoparticles. All these results indicate that this peptide could be a promising therapeutic agent for targeted delivery.http://www.sciencedirect.com/science/article/pii/S2590156722000172NFL-TBS.40-63 peptideSelf-assemblyBIOT-NFL-peptide-nanofibersGold nanoparticlesGlioblastomaTargeted drug delivery |
| spellingShingle | H. Alnemeh-Al Ali A. Griveau F. Artzner A. Dupont N. Lautram M.A. Jourdain J. Eyer Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma International Journal of Pharmaceutics: X NFL-TBS.40-63 peptide Self-assembly BIOT-NFL-peptide-nanofibers Gold nanoparticles Glioblastoma Targeted drug delivery |
| title | Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| title_full | Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| title_fullStr | Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| title_full_unstemmed | Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| title_short | Investigation on the self-assembly of the NFL-TBS.40-63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| title_sort | investigation on the self assembly of the nfl tbs 40 63 peptide and its interaction with gold nanoparticles as a delivery agent for glioblastoma |
| topic | NFL-TBS.40-63 peptide Self-assembly BIOT-NFL-peptide-nanofibers Gold nanoparticles Glioblastoma Targeted drug delivery |
| url | http://www.sciencedirect.com/science/article/pii/S2590156722000172 |
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