The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer
ObjectiveTo evaluate the prognostic value and explore the biological significance of gap junction protein beta 2 (GJB2 or Cx26) in cervical cancer (CC).MethodsWe first compared GJB2 expression between CC and normal tissues using public databases and immunohistochemistry (IHC). Based on The Cancer Ge...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.907960/full |
| _version_ | 1811288128657293312 |
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| author | Silu Meng Silu Meng Yuhuan Liu Yuhuan Liu Xiaoyan Wang Xue Wu Xue Wu Wan Xie Wan Xie Xiaoyan Kang Xiaoyan Kang Xiaoyu Liu Xiaoyu Liu Lili Guo Lili Guo Changyu Wang Changyu Wang |
| author_facet | Silu Meng Silu Meng Yuhuan Liu Yuhuan Liu Xiaoyan Wang Xue Wu Xue Wu Wan Xie Wan Xie Xiaoyan Kang Xiaoyan Kang Xiaoyu Liu Xiaoyu Liu Lili Guo Lili Guo Changyu Wang Changyu Wang |
| author_sort | Silu Meng |
| collection | DOAJ |
| description | ObjectiveTo evaluate the prognostic value and explore the biological significance of gap junction protein beta 2 (GJB2 or Cx26) in cervical cancer (CC).MethodsWe first compared GJB2 expression between CC and normal tissues using public databases and immunohistochemistry (IHC). Based on The Cancer Genome Atlas data (TCGA cohort, n = 304) and tissue microarray samples (OBC cohort, n = 111), we explored the prognostic value of GJB2 for CC patients using bioinformatics analysis and IHC scoring. To explore the biological significance of GJB2, Gene set enrichment analysis (GSEA) and Gene Ontology (GO) were performed. The impact of GJB2 on the immune microenvironment was analyzed by CIBERSORTx and ESTIMATE algorithms. We finally investigated the relationship between GJB2 and drug sensitivity based on the Genomics of Drug Sensitivity in Cancer (GDSC).ResultsThe expression of GJB2 was significantly increased in CC over normal tissues. Both the TCGA and OBC cohort found that patients with high GJB2 expression had shorter overall survival (OS) time, and high GJB2 expression was the independent risk factor for prognosis (TCGA: HR, 2.566; 95% CI, 1.066–6.180; p = 0.036; OBC: HR, 2.198; 95% CI, 1.019–4.741; p = 0.045). GJB2 was correlated with patient clinical factors such as tumor size and differentiation grade. The p53 signaling pathway and toll-like receptor pathway may be regulated by GJB2. The abundance of various immune cells was significantly different between the low and high GJB2 expression groups. The ImmuneScore was significantly increased in the high GJB2 expression group. In addition, the expression level of GJB2 was positively correlated with the natural log of the half-maximal inhibitory concentration (LN_IC50) value of cisplatin/paclitaxel (Spearman r = 0.238/0.153, p < 0.001).ConclusionGJB2 can serve as a potential prognostic marker of poor survival and a therapeutic target in CC. Moreover, GJB2 may affect the immune microenvironment and is correlated with chemoresistance. |
| first_indexed | 2024-04-13T03:31:49Z |
| format | Article |
| id | doaj.art-92bbaefdadf24d6d9d3ba9771689333b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-13T03:31:49Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-92bbaefdadf24d6d9d3ba9771689333b2022-12-22T03:04:28ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-07-011210.3389/fonc.2022.907960907960The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancerSilu Meng0Silu Meng1Yuhuan Liu2Yuhuan Liu3Xiaoyan Wang4Xue Wu5Xue Wu6Wan Xie7Wan Xie8Xiaoyan Kang9Xiaoyan Kang10Xiaoyu Liu11Xiaoyu Liu12Lili Guo13Lili Guo14Changyu Wang15Changyu Wang16Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaObjectiveTo evaluate the prognostic value and explore the biological significance of gap junction protein beta 2 (GJB2 or Cx26) in cervical cancer (CC).MethodsWe first compared GJB2 expression between CC and normal tissues using public databases and immunohistochemistry (IHC). Based on The Cancer Genome Atlas data (TCGA cohort, n = 304) and tissue microarray samples (OBC cohort, n = 111), we explored the prognostic value of GJB2 for CC patients using bioinformatics analysis and IHC scoring. To explore the biological significance of GJB2, Gene set enrichment analysis (GSEA) and Gene Ontology (GO) were performed. The impact of GJB2 on the immune microenvironment was analyzed by CIBERSORTx and ESTIMATE algorithms. We finally investigated the relationship between GJB2 and drug sensitivity based on the Genomics of Drug Sensitivity in Cancer (GDSC).ResultsThe expression of GJB2 was significantly increased in CC over normal tissues. Both the TCGA and OBC cohort found that patients with high GJB2 expression had shorter overall survival (OS) time, and high GJB2 expression was the independent risk factor for prognosis (TCGA: HR, 2.566; 95% CI, 1.066–6.180; p = 0.036; OBC: HR, 2.198; 95% CI, 1.019–4.741; p = 0.045). GJB2 was correlated with patient clinical factors such as tumor size and differentiation grade. The p53 signaling pathway and toll-like receptor pathway may be regulated by GJB2. The abundance of various immune cells was significantly different between the low and high GJB2 expression groups. The ImmuneScore was significantly increased in the high GJB2 expression group. In addition, the expression level of GJB2 was positively correlated with the natural log of the half-maximal inhibitory concentration (LN_IC50) value of cisplatin/paclitaxel (Spearman r = 0.238/0.153, p < 0.001).ConclusionGJB2 can serve as a potential prognostic marker of poor survival and a therapeutic target in CC. Moreover, GJB2 may affect the immune microenvironment and is correlated with chemoresistance.https://www.frontiersin.org/articles/10.3389/fonc.2022.907960/fullGJB2cervical cancerprognostic markerimmune cell abundancechemoresistance |
| spellingShingle | Silu Meng Silu Meng Yuhuan Liu Yuhuan Liu Xiaoyan Wang Xue Wu Xue Wu Wan Xie Wan Xie Xiaoyan Kang Xiaoyan Kang Xiaoyu Liu Xiaoyu Liu Lili Guo Lili Guo Changyu Wang Changyu Wang The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer Frontiers in Oncology GJB2 cervical cancer prognostic marker immune cell abundance chemoresistance |
| title | The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer |
| title_full | The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer |
| title_fullStr | The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer |
| title_full_unstemmed | The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer |
| title_short | The prognostic value and biological significance of gap junction beta protein 2 (GJB2 or Cx26) in cervical cancer |
| title_sort | prognostic value and biological significance of gap junction beta protein 2 gjb2 or cx26 in cervical cancer |
| topic | GJB2 cervical cancer prognostic marker immune cell abundance chemoresistance |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.907960/full |
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