Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens
Abstract The current study aims to investigate the effects of dietary source of n-3 polyunsaturated fatty acids (PUFA) on immune response in broiler chickens, represented by cytotoxic cell activity. A total of 255 one-day-old male Cobb 500 broiler chickens were fed on fish oil (FO)-, flaxseed oil-en...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-89381-3 |
| _version_ | 1819127134200791040 |
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| author | Hanan Al-Khalaifah Afaf Al-Nasser |
| author_facet | Hanan Al-Khalaifah Afaf Al-Nasser |
| author_sort | Hanan Al-Khalaifah |
| collection | DOAJ |
| description | Abstract The current study aims to investigate the effects of dietary source of n-3 polyunsaturated fatty acids (PUFA) on immune response in broiler chickens, represented by cytotoxic cell activity. A total of 255 one-day-old male Cobb 500 broiler chickens were fed on fish oil (FO)-, flaxseed oil-enriched diets at 50 and 19 g/kg, respectively, in addition to the soybean-based control diet. At slaughter, samples of blood and spleen were harvested from 20 birds/treatment (n = 20). The immune tissues' fatty acid profile was analyzed by gas chromatography, and the cytotoxic cell activity was investigated. The results showed that supplementing broiler chickens with diets rich in n-3 PUFA had a substantial effect on the broiler immune tissues' fatty acid profile. Cytotoxic cell activity was significantly higher in splenocytes and peripheral blood mononuclear cells (PBMCs) from broilers fed flaxseed oil than those provided FO and the soybean control diet. These results suggest that flaxseed oil may be used to enrich chickens with n-3 PUFA and improve the immune status of chicken flocks to resist diseases. |
| first_indexed | 2024-12-22T08:07:06Z |
| format | Article |
| id | doaj.art-92bc05daf8474fdebd95f42123cc69ea |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T08:07:06Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-92bc05daf8474fdebd95f42123cc69ea2022-12-21T18:33:06ZengNature PortfolioScientific Reports2045-23222021-05-0111111010.1038/s41598-021-89381-3Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickensHanan Al-Khalaifah0Afaf Al-Nasser1Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research (KISR)Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research (KISR)Abstract The current study aims to investigate the effects of dietary source of n-3 polyunsaturated fatty acids (PUFA) on immune response in broiler chickens, represented by cytotoxic cell activity. A total of 255 one-day-old male Cobb 500 broiler chickens were fed on fish oil (FO)-, flaxseed oil-enriched diets at 50 and 19 g/kg, respectively, in addition to the soybean-based control diet. At slaughter, samples of blood and spleen were harvested from 20 birds/treatment (n = 20). The immune tissues' fatty acid profile was analyzed by gas chromatography, and the cytotoxic cell activity was investigated. The results showed that supplementing broiler chickens with diets rich in n-3 PUFA had a substantial effect on the broiler immune tissues' fatty acid profile. Cytotoxic cell activity was significantly higher in splenocytes and peripheral blood mononuclear cells (PBMCs) from broilers fed flaxseed oil than those provided FO and the soybean control diet. These results suggest that flaxseed oil may be used to enrich chickens with n-3 PUFA and improve the immune status of chicken flocks to resist diseases.https://doi.org/10.1038/s41598-021-89381-3 |
| spellingShingle | Hanan Al-Khalaifah Afaf Al-Nasser Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens Scientific Reports |
| title | Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| title_full | Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| title_fullStr | Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| title_full_unstemmed | Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| title_short | Dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| title_sort | dietary source of polyunsaturated fatty acids influences cell cytotoxicity in broiler chickens |
| url | https://doi.org/10.1038/s41598-021-89381-3 |
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