Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea
To enhance the utilization of summer–autumn tea, a water-soluble polysaccharide (D1N1) was isolated through a series of techniques including hot water extraction, ethanol precipitation, and column chromatography. The structure of D1N1 was determined through the utilization of ultraviolet, Fourier-tr...
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MDPI AG
2024-03-01
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| Series: | Foods |
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| author | Miao Cao Zheng Cao Juanjuan Tian Wenping Lv Hongxin Wang |
| author_facet | Miao Cao Zheng Cao Juanjuan Tian Wenping Lv Hongxin Wang |
| author_sort | Miao Cao |
| collection | DOAJ |
| description | To enhance the utilization of summer–autumn tea, a water-soluble polysaccharide (D1N1) was isolated through a series of techniques including hot water extraction, ethanol precipitation, and column chromatography. The structure of D1N1 was determined through the utilization of ultraviolet, Fourier-transform infrared, high-performance anion-exchange chromatography, gas chromatography–tandem mass spectrometry, and nuclear magnetic resonance. The results revealed that glucose was the predominant component of D1N1, accounting for 95% of its composition. Additionally, D1N1 also contained galactose, arabinose, and rhamnose. The molecular weight (Mw) of D1N1 was determined to be 224.71 kDa. The backbone of D1N1 consisted of →4)-α-D -Glc<i>p</i> (1→, →3,4)-α-D-Gal<i>p</i>-(1→, →4,6)-α-D -Glc<i>p</i> (1→ at a molar ratio of 35:1:1, and branching at the O-3 position of →3,4)-α-D-Gal<i>p</i>-(1→ and O-6 position of →4,6)-α-D-Glc<i>p</i> (1→ with α-D -Glc<i>p</i> (1→. In addition, the antioxidant activity of D1N1 was also evaluated. D1N1 exhibited excellent antioxidant bioactivity against the DPPH, superoxide anion radical, and ABTS<sup>+</sup> radical. These findings provide a theoretical basis for the application of summer–autumn tea polysaccharide as a potential functional food. |
| first_indexed | 2024-04-24T18:17:08Z |
| format | Article |
| id | doaj.art-92bde21a97814ba89ca7e2cce8ebedbc |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-24T18:17:08Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-92bde21a97814ba89ca7e2cce8ebedbc2024-03-27T13:40:23ZengMDPI AGFoods2304-81582024-03-0113682110.3390/foods13060821Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn TeaMiao Cao0Zheng Cao1Juanjuan Tian2Wenping Lv3Hongxin Wang4Department of Tea and Food Science, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaDepartment of Tea and Food Science, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaDepartment of Tea and Food Science, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaSchool of Food Science & Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science & Technology, Jiangnan University, Wuxi 214122, ChinaTo enhance the utilization of summer–autumn tea, a water-soluble polysaccharide (D1N1) was isolated through a series of techniques including hot water extraction, ethanol precipitation, and column chromatography. The structure of D1N1 was determined through the utilization of ultraviolet, Fourier-transform infrared, high-performance anion-exchange chromatography, gas chromatography–tandem mass spectrometry, and nuclear magnetic resonance. The results revealed that glucose was the predominant component of D1N1, accounting for 95% of its composition. Additionally, D1N1 also contained galactose, arabinose, and rhamnose. The molecular weight (Mw) of D1N1 was determined to be 224.71 kDa. The backbone of D1N1 consisted of →4)-α-D -Glc<i>p</i> (1→, →3,4)-α-D-Gal<i>p</i>-(1→, →4,6)-α-D -Glc<i>p</i> (1→ at a molar ratio of 35:1:1, and branching at the O-3 position of →3,4)-α-D-Gal<i>p</i>-(1→ and O-6 position of →4,6)-α-D-Glc<i>p</i> (1→ with α-D -Glc<i>p</i> (1→. In addition, the antioxidant activity of D1N1 was also evaluated. D1N1 exhibited excellent antioxidant bioactivity against the DPPH, superoxide anion radical, and ABTS<sup>+</sup> radical. These findings provide a theoretical basis for the application of summer–autumn tea polysaccharide as a potential functional food.https://www.mdpi.com/2304-8158/13/6/821summer–autumn teapolysaccharidestructural characterizationantioxidant activity |
| spellingShingle | Miao Cao Zheng Cao Juanjuan Tian Wenping Lv Hongxin Wang Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea Foods summer–autumn tea polysaccharide structural characterization antioxidant activity |
| title | Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea |
| title_full | Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea |
| title_fullStr | Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea |
| title_full_unstemmed | Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea |
| title_short | Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea |
| title_sort | structural characterization and in vitro antioxidant activity of a novel polysaccharide from summer autumn tea |
| topic | summer–autumn tea polysaccharide structural characterization antioxidant activity |
| url | https://www.mdpi.com/2304-8158/13/6/821 |
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