Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy
The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaugh...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2016-11-01
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| Series: | Italian Journal of Food Safety |
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| Online Access: | http://www.pagepressjournals.org/index.php/ijfs/article/view/6151 |
| _version_ | 1818435786705469440 |
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| author | Pierluigi Di Ciccio Maria Cristina Ossiprandi Emanuela Zanardi Sergio Ghidini Giancarlo Belluzzi Alberto Vergara Adriana Ianieri |
| author_facet | Pierluigi Di Ciccio Maria Cristina Ossiprandi Emanuela Zanardi Sergio Ghidini Giancarlo Belluzzi Alberto Vergara Adriana Ianieri |
| author_sort | Pierluigi Di Ciccio |
| collection | DOAJ |
| description | The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected. Samples from pig carcasses and slaughterhouse environment were analyzed for total viable count (TVC), <em>Enterobacteriaceae</em> count (EBC) and <em>Salmonella</em>. The caecal contents were examined for <em>Salmonella</em>. Carcasses from slaughterhouse A presented the greatest TVC and EBC mean log value, whereas environmental samples collected during slaughter activities from slaughterhouse C showed the greatest TVC and EBC mean log value. As far as the environmental samples collected before slaughter activities are concerned, an average up to 6 log<sub>10</sub> colony forming unit (CFU)/cm<sup>2</sup> TVC in two slaughter plants (A and C) and 5 log<sub>10</sub> CFU/cm<sup>2</sup> TVC in one slaughter plant (B) was detected. <em>Salmonella</em> was recovered in two slaughterhouses (A and B). Four different <em>Salmonella</em> serotypes were detected in the positive samples (11). Within serotype <em>S.</em> Rissen and <em>S.</em> Typhimurium monophasic-variant isolates, two pulsed-field gel electrophoresis patterns were identified. The findings in this survey suggest that carcass contamination is influenced by the slaughterhouse plant and this could be a result of differences in line speed. The results of environmental sampling have not shown an association with the slaughterhouse plant. |
| first_indexed | 2024-12-14T16:58:25Z |
| format | Article |
| id | doaj.art-92c45abfcdc646f8a4ea78892880ce7b |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-12-14T16:58:25Z |
| publishDate | 2016-11-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-92c45abfcdc646f8a4ea78892880ce7b2022-12-21T22:53:54ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322016-11-015410.4081/ijfs.2016.61514983Microbiological contamination in three large-scale pig slaughterhouses in Northern ItalyPierluigi Di Ciccio0Maria Cristina Ossiprandi1Emanuela Zanardi2Sergio Ghidini3Giancarlo Belluzzi4Alberto Vergara5Adriana Ianieri6Department of Food Science, University of Parma, ParmaDepartment of Veterinary Science, University of ParmaDepartment of Food Science, University of Parma, ParmaDepartment of Food Science, University of Parma, ParmaMinistry of Health, ParmaFaculty of Veterinary Medicine, University of Teramo, TeramoDepartment of Food Science, University of Parma, ParmaThe aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected. Samples from pig carcasses and slaughterhouse environment were analyzed for total viable count (TVC), <em>Enterobacteriaceae</em> count (EBC) and <em>Salmonella</em>. The caecal contents were examined for <em>Salmonella</em>. Carcasses from slaughterhouse A presented the greatest TVC and EBC mean log value, whereas environmental samples collected during slaughter activities from slaughterhouse C showed the greatest TVC and EBC mean log value. As far as the environmental samples collected before slaughter activities are concerned, an average up to 6 log<sub>10</sub> colony forming unit (CFU)/cm<sup>2</sup> TVC in two slaughter plants (A and C) and 5 log<sub>10</sub> CFU/cm<sup>2</sup> TVC in one slaughter plant (B) was detected. <em>Salmonella</em> was recovered in two slaughterhouses (A and B). Four different <em>Salmonella</em> serotypes were detected in the positive samples (11). Within serotype <em>S.</em> Rissen and <em>S.</em> Typhimurium monophasic-variant isolates, two pulsed-field gel electrophoresis patterns were identified. The findings in this survey suggest that carcass contamination is influenced by the slaughterhouse plant and this could be a result of differences in line speed. The results of environmental sampling have not shown an association with the slaughterhouse plant.http://www.pagepressjournals.org/index.php/ijfs/article/view/6151Pig slaughterhousePig carcassTotal viable countEnterobacteriaceaeSalmonella |
| spellingShingle | Pierluigi Di Ciccio Maria Cristina Ossiprandi Emanuela Zanardi Sergio Ghidini Giancarlo Belluzzi Alberto Vergara Adriana Ianieri Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy Italian Journal of Food Safety Pig slaughterhouse Pig carcass Total viable count Enterobacteriaceae Salmonella |
| title | Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy |
| title_full | Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy |
| title_fullStr | Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy |
| title_full_unstemmed | Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy |
| title_short | Microbiological contamination in three large-scale pig slaughterhouses in Northern Italy |
| title_sort | microbiological contamination in three large scale pig slaughterhouses in northern italy |
| topic | Pig slaughterhouse Pig carcass Total viable count Enterobacteriaceae Salmonella |
| url | http://www.pagepressjournals.org/index.php/ijfs/article/view/6151 |
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