Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis.
Despite high rates of exposure, only 5-10% of people infected with Mycobacterium tuberculosis will develop active tuberculosis (TB) disease, suggesting a significant role for genetic variation in the human immune response to this infection. Here, we studied TB association and expression of 18 genes...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-10-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2568981?pdf=render |
| _version_ | 1818136481655422976 |
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| author | Sonia Davila Martin L Hibberd Ranjeeta Hari Dass Hazel E E Wong Edhyana Sahiratmadja Carine Bonnard Bachti Alisjahbana Jeffrey S Szeszko Yanina Balabanova Francis Drobniewski Reinout van Crevel Esther van de Vosse Sergey Nejentsev Tom H M Ottenhoff Mark Seielstad |
| author_facet | Sonia Davila Martin L Hibberd Ranjeeta Hari Dass Hazel E E Wong Edhyana Sahiratmadja Carine Bonnard Bachti Alisjahbana Jeffrey S Szeszko Yanina Balabanova Francis Drobniewski Reinout van Crevel Esther van de Vosse Sergey Nejentsev Tom H M Ottenhoff Mark Seielstad |
| author_sort | Sonia Davila |
| collection | DOAJ |
| description | Despite high rates of exposure, only 5-10% of people infected with Mycobacterium tuberculosis will develop active tuberculosis (TB) disease, suggesting a significant role for genetic variation in the human immune response to this infection. Here, we studied TB association and expression of 18 genes involved in the Toll-like receptor (TLR) pathways. Initially, we genotyped 149 sequence polymorphisms in 375 pulmonary TB patients and 387 controls from Indonesia. We found that four polymorphisms in the TLR8 gene on chromosome X showed evidence of association with TB susceptibility in males, including a non-synonymous polymorphism rs3764880 (Met1Val; P = 0.007, odds ratio (OR) = 1.8, 95% c.i. = 1.2-2.7). We genotyped these four TLR8 polymorphisms in an independent collection of 1,837 pulmonary TB patients and 1,779 controls from Russia and again found evidence of association in males (for rs3764880 P = 0.03, OR = 1.2, 95% c.i. = 1.02-1.48). Combined evidence for association is P = 1.2x10(-3)-6x10(-4). In addition, a quantitative PCR analysis indicated that TLR8 transcript levels are significantly up-regulated in patients during the acute phase of disease (P = 9.36x10(-5)), relative to baseline levels following successful chemotherapy. A marked increase in TLR8 protein expression was also observed directly in differentiated macrophages upon infection with M. bovis bacille Calmette-Guérin (BCG). Taken together, our results provide evidence, for the first time, of a role for the TLR8 gene in susceptibility to pulmonary TB across different populations. |
| first_indexed | 2024-12-11T09:41:06Z |
| format | Article |
| id | doaj.art-92c7c158cba54fe682ce14d2f76b2fb0 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T09:41:06Z |
| publishDate | 2008-10-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj.art-92c7c158cba54fe682ce14d2f76b2fb02022-12-22T01:12:41ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042008-10-01410e100021810.1371/journal.pgen.1000218Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis.Sonia DavilaMartin L HibberdRanjeeta Hari DassHazel E E WongEdhyana SahiratmadjaCarine BonnardBachti AlisjahbanaJeffrey S SzeszkoYanina BalabanovaFrancis DrobniewskiReinout van CrevelEsther van de VosseSergey NejentsevTom H M OttenhoffMark SeielstadDespite high rates of exposure, only 5-10% of people infected with Mycobacterium tuberculosis will develop active tuberculosis (TB) disease, suggesting a significant role for genetic variation in the human immune response to this infection. Here, we studied TB association and expression of 18 genes involved in the Toll-like receptor (TLR) pathways. Initially, we genotyped 149 sequence polymorphisms in 375 pulmonary TB patients and 387 controls from Indonesia. We found that four polymorphisms in the TLR8 gene on chromosome X showed evidence of association with TB susceptibility in males, including a non-synonymous polymorphism rs3764880 (Met1Val; P = 0.007, odds ratio (OR) = 1.8, 95% c.i. = 1.2-2.7). We genotyped these four TLR8 polymorphisms in an independent collection of 1,837 pulmonary TB patients and 1,779 controls from Russia and again found evidence of association in males (for rs3764880 P = 0.03, OR = 1.2, 95% c.i. = 1.02-1.48). Combined evidence for association is P = 1.2x10(-3)-6x10(-4). In addition, a quantitative PCR analysis indicated that TLR8 transcript levels are significantly up-regulated in patients during the acute phase of disease (P = 9.36x10(-5)), relative to baseline levels following successful chemotherapy. A marked increase in TLR8 protein expression was also observed directly in differentiated macrophages upon infection with M. bovis bacille Calmette-Guérin (BCG). Taken together, our results provide evidence, for the first time, of a role for the TLR8 gene in susceptibility to pulmonary TB across different populations.http://europepmc.org/articles/PMC2568981?pdf=render |
| spellingShingle | Sonia Davila Martin L Hibberd Ranjeeta Hari Dass Hazel E E Wong Edhyana Sahiratmadja Carine Bonnard Bachti Alisjahbana Jeffrey S Szeszko Yanina Balabanova Francis Drobniewski Reinout van Crevel Esther van de Vosse Sergey Nejentsev Tom H M Ottenhoff Mark Seielstad Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. PLoS Genetics |
| title | Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. |
| title_full | Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. |
| title_fullStr | Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. |
| title_full_unstemmed | Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. |
| title_short | Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. |
| title_sort | genetic association and expression studies indicate a role of toll like receptor 8 in pulmonary tuberculosis |
| url | http://europepmc.org/articles/PMC2568981?pdf=render |
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