Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids
Objective To investigate the histological differences and expression of E-cadherin, Vimentin, matrix metalloprotein-9 (MMP-9), TNF-α, dipeptidyl-peptidase 4 (DPP4, also called CD26) in the margin and center of hypertrophic scar, superficial dilated keloid and normal skin. Methods Ten patients with k...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Army Medical University
2022-11-01
|
| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202203103.htm |
| _version_ | 1828198578558861312 |
|---|---|
| author | GONG Wanru LIU Mengchang LIU Xingke XIE Defu YAN Hong |
| author_facet | GONG Wanru LIU Mengchang LIU Xingke XIE Defu YAN Hong |
| author_sort | GONG Wanru |
| collection | DOAJ |
| description | Objective To investigate the histological differences and expression of E-cadherin, Vimentin, matrix metalloprotein-9 (MMP-9), TNF-α, dipeptidyl-peptidase 4 (DPP4, also called CD26) in the margin and center of hypertrophic scar, superficial dilated keloid and normal skin. Methods Ten patients with keloid and another 10 with hypertrophic scar undergoing excision surgery in our hospital from October 2020 to August 2021 were included, and 8 cases requiring partial normal skin resection were also recruited. The resected tissues were collected, the tissue morphology was observed with HE staining, and the expression levels of E-cadherin, Vimentin, MMP-9, TNF-α and CD26 were detected by immunohistochemical staining and Western blotting. Results The E-cadherin expression in the epidermis of both keloid and hypertrophic scar was significantly lower than that of normal skin (P < 0.05), and the level in the edge of keloid was obviously weakened as compared with that of the center (P < 0.05), but there was no significant difference between the edge of hypertrophic scar and the center (P>0.05). By contrast, the expression levels of Vimentin, MMP-9, TNF-α and CD26 in the epidermis of both keloid and hypertrophic scar were remarkably enhanced when compared with those of normal skin (P < 0.05), and the keloids' edge higher than the center (P < 0.05), while no significance between the hypertrophic scars' edge and center (P>0.05). Conclusion The differences in epithelial-to-mesenchymal transition (EMT) and expression of relevant biomarkers within individual scars may be one of the reasons for the diversity of clinical features between hypertrophic scar and keloid, in which TNF-α and CD26 may be the key factors.
|
| first_indexed | 2024-04-12T10:40:24Z |
| format | Article |
| id | doaj.art-92cd7b46f107475b9b55ab91b1bfa6f1 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-12T10:40:24Z |
| publishDate | 2022-11-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-92cd7b46f107475b9b55ab91b1bfa6f12022-12-22T03:36:37ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272022-11-0144212174218210.16016/j.2097-0927.202203103Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloidsGONG Wanru0LIU Mengchang1LIU Xingke2XIE Defu3YAN Hong4Department of Plastic and Burn Surgery, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, 646000, ChinaDepartment of Plastic and Burn Surgery, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, 646000, ChinaDepartment of Plastic and Burn Surgery, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, 646000, ChinaDepartment of Plastic and Burn Surgery, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, 646000, ChinaDepartment of Plastic and Burn Surgery, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, 646000, ChinaObjective To investigate the histological differences and expression of E-cadherin, Vimentin, matrix metalloprotein-9 (MMP-9), TNF-α, dipeptidyl-peptidase 4 (DPP4, also called CD26) in the margin and center of hypertrophic scar, superficial dilated keloid and normal skin. Methods Ten patients with keloid and another 10 with hypertrophic scar undergoing excision surgery in our hospital from October 2020 to August 2021 were included, and 8 cases requiring partial normal skin resection were also recruited. The resected tissues were collected, the tissue morphology was observed with HE staining, and the expression levels of E-cadherin, Vimentin, MMP-9, TNF-α and CD26 were detected by immunohistochemical staining and Western blotting. Results The E-cadherin expression in the epidermis of both keloid and hypertrophic scar was significantly lower than that of normal skin (P < 0.05), and the level in the edge of keloid was obviously weakened as compared with that of the center (P < 0.05), but there was no significant difference between the edge of hypertrophic scar and the center (P>0.05). By contrast, the expression levels of Vimentin, MMP-9, TNF-α and CD26 in the epidermis of both keloid and hypertrophic scar were remarkably enhanced when compared with those of normal skin (P < 0.05), and the keloids' edge higher than the center (P < 0.05), while no significance between the hypertrophic scars' edge and center (P>0.05). Conclusion The differences in epithelial-to-mesenchymal transition (EMT) and expression of relevant biomarkers within individual scars may be one of the reasons for the diversity of clinical features between hypertrophic scar and keloid, in which TNF-α and CD26 may be the key factors. http://aammt.tmmu.edu.cn/Upload/rhtml/202203103.htmhypertrophic scarkeloidclinical manifestationepithelial-to-mesenchymal transitiontnf-αcd26 |
| spellingShingle | GONG Wanru LIU Mengchang LIU Xingke XIE Defu YAN Hong Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids 陆军军医大学学报 hypertrophic scar keloid clinical manifestation epithelial-to-mesenchymal transition tnf-α cd26 |
| title | Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids |
| title_full | Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids |
| title_fullStr | Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids |
| title_full_unstemmed | Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids |
| title_short | Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids |
| title_sort | heterogeneity of epithelial mesenchymal transition and cd26 expression in hypertrophic scars and keloids |
| topic | hypertrophic scar keloid clinical manifestation epithelial-to-mesenchymal transition tnf-α cd26 |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202203103.htm |
| work_keys_str_mv | AT gongwanru heterogeneityofepithelialmesenchymaltransitionandcd26expressioninhypertrophicscarsandkeloids AT liumengchang heterogeneityofepithelialmesenchymaltransitionandcd26expressioninhypertrophicscarsandkeloids AT liuxingke heterogeneityofepithelialmesenchymaltransitionandcd26expressioninhypertrophicscarsandkeloids AT xiedefu heterogeneityofepithelialmesenchymaltransitionandcd26expressioninhypertrophicscarsandkeloids AT yanhong heterogeneityofepithelialmesenchymaltransitionandcd26expressioninhypertrophicscarsandkeloids |