Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces
User-friendly devices for detecting low gliadin content in commercial foods are of extreme importance for people with gluten diseases. With this concern, the present work proposes a rapid and sensitive optical nanostructured microarrays platform for the detection of gliadin using specific anti-gliad...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/10/2/38 |
| _version_ | 1797481777166548992 |
|---|---|
| author | Giuliocesare Casari Bariani Lan Zhou Simone Poggesi Marisa Manzano Rodica Elena Ionescu |
| author_facet | Giuliocesare Casari Bariani Lan Zhou Simone Poggesi Marisa Manzano Rodica Elena Ionescu |
| author_sort | Giuliocesare Casari Bariani |
| collection | DOAJ |
| description | User-friendly devices for detecting low gliadin content in commercial foods are of extreme importance for people with gluten diseases. With this concern, the present work proposes a rapid and sensitive optical nanostructured microarrays platform for the detection of gliadin using specific anti-gliadin IgG antibodies immobilized on annealed gold nanostructures (AuNPs) obtained after the high annealing process (550 °C) of gold thin films evaporated on commercial glass coverslips. Localized Surface Plasmon Resonance (LSPR) immunosensing of gliadin in the range of 0.1 ppm to 1000 ppm is successfully achieved. In addition, the biofunctionalization protocol was used for gluten screening in five food complex products. |
| first_indexed | 2024-03-09T22:19:23Z |
| format | Article |
| id | doaj.art-92cf72d0c69a4ede9d406fabddec6b2c |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T22:19:23Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-92cf72d0c69a4ede9d406fabddec6b2c2023-11-23T19:16:54ZengMDPI AGChemosensors2227-90402022-01-011023810.3390/chemosensors10020038Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin TracesGiuliocesare Casari Bariani0Lan Zhou1Simone Poggesi2Marisa Manzano3Rodica Elena Ionescu4Light, Nanomaterials and Nanotechnology (L2n) Laboratory, CNRS EMR 7004, University of Technology of Troyes, 12 Rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, FranceLight, Nanomaterials and Nanotechnology (L2n) Laboratory, CNRS EMR 7004, University of Technology of Troyes, 12 Rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, FranceLight, Nanomaterials and Nanotechnology (L2n) Laboratory, CNRS EMR 7004, University of Technology of Troyes, 12 Rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, FranceDipartimento di Scienze Agroalimentari, Ambientali e Animali (DI4A), Università degli Studi di Udine, Via Sondrio 2/A, 33100 Udine, ItalyLight, Nanomaterials and Nanotechnology (L2n) Laboratory, CNRS EMR 7004, University of Technology of Troyes, 12 Rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, FranceUser-friendly devices for detecting low gliadin content in commercial foods are of extreme importance for people with gluten diseases. With this concern, the present work proposes a rapid and sensitive optical nanostructured microarrays platform for the detection of gliadin using specific anti-gliadin IgG antibodies immobilized on annealed gold nanostructures (AuNPs) obtained after the high annealing process (550 °C) of gold thin films evaporated on commercial glass coverslips. Localized Surface Plasmon Resonance (LSPR) immunosensing of gliadin in the range of 0.1 ppm to 1000 ppm is successfully achieved. In addition, the biofunctionalization protocol was used for gluten screening in five food complex products.https://www.mdpi.com/2227-9040/10/2/38glass coverslipgliadin in foodsTEM-gridsannealed gold nanostructuresdroplet biofunctionalizationLSPR immunosensor |
| spellingShingle | Giuliocesare Casari Bariani Lan Zhou Simone Poggesi Marisa Manzano Rodica Elena Ionescu Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces Chemosensors glass coverslip gliadin in foods TEM-grids annealed gold nanostructures droplet biofunctionalization LSPR immunosensor |
| title | Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces |
| title_full | Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces |
| title_fullStr | Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces |
| title_full_unstemmed | Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces |
| title_short | Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces |
| title_sort | patterning large scale nanostructured microarrays on coverslip for sensitive plasmonic detection of aqueous gliadin traces |
| topic | glass coverslip gliadin in foods TEM-grids annealed gold nanostructures droplet biofunctionalization LSPR immunosensor |
| url | https://www.mdpi.com/2227-9040/10/2/38 |
| work_keys_str_mv | AT giuliocesarecasaribariani patterninglargescalenanostructuredmicroarraysoncoverslipforsensitiveplasmonicdetectionofaqueousgliadintraces AT lanzhou patterninglargescalenanostructuredmicroarraysoncoverslipforsensitiveplasmonicdetectionofaqueousgliadintraces AT simonepoggesi patterninglargescalenanostructuredmicroarraysoncoverslipforsensitiveplasmonicdetectionofaqueousgliadintraces AT marisamanzano patterninglargescalenanostructuredmicroarraysoncoverslipforsensitiveplasmonicdetectionofaqueousgliadintraces AT rodicaelenaionescu patterninglargescalenanostructuredmicroarraysoncoverslipforsensitiveplasmonicdetectionofaqueousgliadintraces |