Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435
Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5′ position is the simplest way to develop nucleobase-based structures by transesterification using...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/13/4112 |
| _version_ | 1797442580614479872 |
|---|---|
| author | Sany Chea Khac Toan Nguyen Ruben R. Rosencrantz |
| author_facet | Sany Chea Khac Toan Nguyen Ruben R. Rosencrantz |
| author_sort | Sany Chea |
| collection | DOAJ |
| description | Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5′ position is the simplest way to develop nucleobase-based structures by transesterification using the lipase Novozym 435. Herein, we describe the optimization of the lipase-catalyzed synthesis of the monomer 5′-<i>O</i>-methacryloylcytidine with the assistance of microwave irradiation. Variable reaction parameters, such as enzyme concentration, molar ratio of the substrate, reaction temperature and reaction time, were investigated to find the optimum reaction condition in terms of obtaining the highest yield. |
| first_indexed | 2024-03-09T12:43:56Z |
| format | Article |
| id | doaj.art-92cfce308f224e5695a5a42f0d35dcf8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:43:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-92cfce308f224e5695a5a42f0d35dcf82023-11-30T22:14:12ZengMDPI AGMolecules1420-30492022-06-012713411210.3390/molecules27134112Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435Sany Chea0Khac Toan Nguyen1Ruben R. Rosencrantz2Fraunhofer Institute of Applied Polymer Research, Biofunctionalized Materials and (Glyco) Biotechnology, Geiselbergstr. 69, 14476 Potsdam, GermanyFraunhofer Institute of Applied Polymer Research, Biofunctionalized Materials and (Glyco) Biotechnology, Geiselbergstr. 69, 14476 Potsdam, GermanyFraunhofer Institute of Applied Polymer Research, Biofunctionalized Materials and (Glyco) Biotechnology, Geiselbergstr. 69, 14476 Potsdam, GermanyNucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5′ position is the simplest way to develop nucleobase-based structures by transesterification using the lipase Novozym 435. Herein, we describe the optimization of the lipase-catalyzed synthesis of the monomer 5′-<i>O</i>-methacryloylcytidine with the assistance of microwave irradiation. Variable reaction parameters, such as enzyme concentration, molar ratio of the substrate, reaction temperature and reaction time, were investigated to find the optimum reaction condition in terms of obtaining the highest yield.https://www.mdpi.com/1420-3049/27/13/4112microwave irradiationNovozym 435cytidinemonomersmart materials |
| spellingShingle | Sany Chea Khac Toan Nguyen Ruben R. Rosencrantz Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 Molecules microwave irradiation Novozym 435 cytidine monomer smart materials |
| title | Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 |
| title_full | Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 |
| title_fullStr | Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 |
| title_full_unstemmed | Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 |
| title_short | Microwave-Assisted Synthesis of 5′-<em>O</em>-methacryloylcytidine Using the Immobilized Lipase Novozym 435 |
| title_sort | microwave assisted synthesis of 5 em o em methacryloylcytidine using the immobilized lipase novozym 435 |
| topic | microwave irradiation Novozym 435 cytidine monomer smart materials |
| url | https://www.mdpi.com/1420-3049/27/13/4112 |
| work_keys_str_mv | AT sanychea microwaveassistedsynthesisof5emoemmethacryloylcytidineusingtheimmobilizedlipasenovozym435 AT khactoannguyen microwaveassistedsynthesisof5emoemmethacryloylcytidineusingtheimmobilizedlipasenovozym435 AT rubenrrosencrantz microwaveassistedsynthesisof5emoemmethacryloylcytidineusingtheimmobilizedlipasenovozym435 |