Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall
Rabbits, pivotal in the EU as livestock, pets, and experimental animals, face bacterial infection challenges, prompting a quest for alternatives to curb antibiotic resistance. Bovine colostrum (BC), rich in immunoregulatory compounds, antimicrobial peptides, and growth factors, is explored for disea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/5/800 |
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| author | Federica Riva Susanna Draghi Alessia Inglesi Joel Filipe Paola Cremonesi Antonio Lavazza Patrizia Cavadini Daniele Vigo Stella Agradi Laura Menchetti Alessia Di Giancamillo Lucia Aidos Silvia Clotilde Modina Nour Elhouda Fehri Grazia Pastorelli Valentina Serra Claudia Maria Balzaretti Marta Castrica Marco Severgnini Gabriele Brecchia Giulio Curone |
| author_facet | Federica Riva Susanna Draghi Alessia Inglesi Joel Filipe Paola Cremonesi Antonio Lavazza Patrizia Cavadini Daniele Vigo Stella Agradi Laura Menchetti Alessia Di Giancamillo Lucia Aidos Silvia Clotilde Modina Nour Elhouda Fehri Grazia Pastorelli Valentina Serra Claudia Maria Balzaretti Marta Castrica Marco Severgnini Gabriele Brecchia Giulio Curone |
| author_sort | Federica Riva |
| collection | DOAJ |
| description | Rabbits, pivotal in the EU as livestock, pets, and experimental animals, face bacterial infection challenges, prompting a quest for alternatives to curb antibiotic resistance. Bovine colostrum (BC), rich in immunoregulatory compounds, antimicrobial peptides, and growth factors, is explored for disease treatment and prevention. This study assesses BC diet supplementation effects on rabbit intestines, examining gene expression. Thirty female New Zealand White rabbits at weaning (35 days) were divided into three experimental groups: control (commercial feed), 2.5% BC, and 5% BC. The diets were administered until slaughtering (81 days). BC-upregulated genes in the jejunum included IL-8, TGF-β, and CTNN-β1 at 5% BC, while PLVAP at 2.5% BC. Antioxidant-related genes (SOD1, GSR) were downregulated in the cecum and colon with 2.5% BC. BC 5% promoted IL-8 in the jejunum, fostering inflammation and immune cell migration. It also induced genes regulating inflammatory responses (TGF-β) and gastrointestinal permeability (CTNN-β1). BC 5% enhanced antioxidant activity in the cecum and colon, but no significant impact on anti-myxo antibody production was observed. These results suggest that BC has significant effects on the rabbit gastrointestinal tract’s inflammatory and antioxidant response, but further research is required to fully understand its histological and physiological impact. |
| first_indexed | 2024-04-25T00:36:41Z |
| format | Article |
| id | doaj.art-92d0b862f22e49d48fa1c3d51b9d31d7 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-25T00:36:41Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-92d0b862f22e49d48fa1c3d51b9d31d72024-03-12T16:38:22ZengMDPI AGAnimals2076-26152024-03-0114580010.3390/ani14050800Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut WallFederica Riva0Susanna Draghi1Alessia Inglesi2Joel Filipe3Paola Cremonesi4Antonio Lavazza5Patrizia Cavadini6Daniele Vigo7Stella Agradi8Laura Menchetti9Alessia Di Giancamillo10Lucia Aidos11Silvia Clotilde Modina12Nour Elhouda Fehri13Grazia Pastorelli14Valentina Serra15Claudia Maria Balzaretti16Marta Castrica17Marco Severgnini18Gabriele Brecchia19Giulio Curone20Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyIstituto di Biologia e Biotecnologia Agraria (IBBA), National Research Council (CNR), Via Einstein, 26900 Lodi, ItalyVirology Laboratory, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Bianchi 9, 25124 Brescia, ItalyVirology Laboratory, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Bianchi 9, 25124 Brescia, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93–95, 62024 Matelica, ItalyDepartment of Biomedical Sciences for Health, University of Milan, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDipartimento di Biomedicina Comparata e Alimentazione—BCA, University of Padua, Viale dell’Università 16, 35020 Legnaro, ItalyInstitute of Biomedical Technologies (ITB), National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyRabbits, pivotal in the EU as livestock, pets, and experimental animals, face bacterial infection challenges, prompting a quest for alternatives to curb antibiotic resistance. Bovine colostrum (BC), rich in immunoregulatory compounds, antimicrobial peptides, and growth factors, is explored for disease treatment and prevention. This study assesses BC diet supplementation effects on rabbit intestines, examining gene expression. Thirty female New Zealand White rabbits at weaning (35 days) were divided into three experimental groups: control (commercial feed), 2.5% BC, and 5% BC. The diets were administered until slaughtering (81 days). BC-upregulated genes in the jejunum included IL-8, TGF-β, and CTNN-β1 at 5% BC, while PLVAP at 2.5% BC. Antioxidant-related genes (SOD1, GSR) were downregulated in the cecum and colon with 2.5% BC. BC 5% promoted IL-8 in the jejunum, fostering inflammation and immune cell migration. It also induced genes regulating inflammatory responses (TGF-β) and gastrointestinal permeability (CTNN-β1). BC 5% enhanced antioxidant activity in the cecum and colon, but no significant impact on anti-myxo antibody production was observed. These results suggest that BC has significant effects on the rabbit gastrointestinal tract’s inflammatory and antioxidant response, but further research is required to fully understand its histological and physiological impact.https://www.mdpi.com/2076-2615/14/5/800nutraceuticalbovine colostrumgene expression |
| spellingShingle | Federica Riva Susanna Draghi Alessia Inglesi Joel Filipe Paola Cremonesi Antonio Lavazza Patrizia Cavadini Daniele Vigo Stella Agradi Laura Menchetti Alessia Di Giancamillo Lucia Aidos Silvia Clotilde Modina Nour Elhouda Fehri Grazia Pastorelli Valentina Serra Claudia Maria Balzaretti Marta Castrica Marco Severgnini Gabriele Brecchia Giulio Curone Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall Animals nutraceutical bovine colostrum gene expression |
| title | Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall |
| title_full | Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall |
| title_fullStr | Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall |
| title_full_unstemmed | Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall |
| title_short | Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut–Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall |
| title_sort | bovine colostrum supplementation in rabbit diet modulates gene expression of cytokines gut vascular barrier and red ox related molecules in the gut wall |
| topic | nutraceutical bovine colostrum gene expression |
| url | https://www.mdpi.com/2076-2615/14/5/800 |
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