Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants
Drought stress profoundly affects plant growth and development, posing a significant challenge that is extensively researched in the field. Thioredoxins (TRXs), small proteins central to redox processes, are crucial to managing both abiotic and biotic stresses. In this research, the <i>VyTRXy&...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16388 |
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| author | Jiang Xiang Min Li Yiyi Li Yi Liu Lingzhu Wei Ting Zheng Jiang Wu Yihe Yu Jianhui Cheng |
| author_facet | Jiang Xiang Min Li Yiyi Li Yi Liu Lingzhu Wei Ting Zheng Jiang Wu Yihe Yu Jianhui Cheng |
| author_sort | Jiang Xiang |
| collection | DOAJ |
| description | Drought stress profoundly affects plant growth and development, posing a significant challenge that is extensively researched in the field. Thioredoxins (TRXs), small proteins central to redox processes, are crucial to managing both abiotic and biotic stresses. In this research, the <i>VyTRXy</i> gene, cloned from wild Yanshan grapes, was validated as a functional TRX through enzyme activity assays. VyTRXy was found to bolster photosynthesis, augment levels of osmotic regulators, stimulate antioxidant enzyme activities, and strengthen drought resilience in transgenic plants. These enhancements were evidenced by higher survival rates, optimized photosynthetic metrics, increased proline levels, augmented chlorophyll concentration, reduced electrolyte leakage, and decreased malondialdehyde and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels. Furthermore, there was a surge in the activities of enzymes such as catalase, ascorbate peroxidase, glutathione peroxidase, dehydroascorbate reductase, and glutathione reductase, along with an increased expression of TRX peroxidase. Notably, under drought stress, there was a marked elevation in the expression of stress-responsive genes, including the adversity stress-inducible expression gene (<i>NtRD29A</i>) and DRE-binding protein (NtDREB), in transgenic tobacco. This investigation is pivotal in the quest for drought-resistant grapevine varieties and provides significant insights into the molecular functionality of <i>VyTRXy</i> in enhancing grapevine drought tolerance. |
| first_indexed | 2024-03-09T16:45:10Z |
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| id | doaj.art-92d1f2a98eb046aaa5b867c92fee55f7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:10Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-92d1f2a98eb046aaa5b867c92fee55f72023-11-24T14:47:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221638810.3390/ijms242216388Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of PlantsJiang Xiang0Min Li1Yiyi Li2Yi Liu3Lingzhu Wei4Ting Zheng5Jiang Wu6Yihe Yu7Jianhui Cheng8Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaHenan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, College of Horticultural and Plant Protection, Henan University of Science and Technology, Luoyang 471023, ChinaHenan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, College of Horticultural and Plant Protection, Henan University of Science and Technology, Luoyang 471023, ChinaHenan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, College of Horticultural and Plant Protection, Henan University of Science and Technology, Luoyang 471023, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaHenan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, College of Horticultural and Plant Protection, Henan University of Science and Technology, Luoyang 471023, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaDrought stress profoundly affects plant growth and development, posing a significant challenge that is extensively researched in the field. Thioredoxins (TRXs), small proteins central to redox processes, are crucial to managing both abiotic and biotic stresses. In this research, the <i>VyTRXy</i> gene, cloned from wild Yanshan grapes, was validated as a functional TRX through enzyme activity assays. VyTRXy was found to bolster photosynthesis, augment levels of osmotic regulators, stimulate antioxidant enzyme activities, and strengthen drought resilience in transgenic plants. These enhancements were evidenced by higher survival rates, optimized photosynthetic metrics, increased proline levels, augmented chlorophyll concentration, reduced electrolyte leakage, and decreased malondialdehyde and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels. Furthermore, there was a surge in the activities of enzymes such as catalase, ascorbate peroxidase, glutathione peroxidase, dehydroascorbate reductase, and glutathione reductase, along with an increased expression of TRX peroxidase. Notably, under drought stress, there was a marked elevation in the expression of stress-responsive genes, including the adversity stress-inducible expression gene (<i>NtRD29A</i>) and DRE-binding protein (NtDREB), in transgenic tobacco. This investigation is pivotal in the quest for drought-resistant grapevine varieties and provides significant insights into the molecular functionality of <i>VyTRXy</i> in enhancing grapevine drought tolerance.https://www.mdpi.com/1422-0067/24/22/16388grapevine<i>VyTRXy</i>droughtphotosynthesis |
| spellingShingle | Jiang Xiang Min Li Yiyi Li Yi Liu Lingzhu Wei Ting Zheng Jiang Wu Yihe Yu Jianhui Cheng Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants International Journal of Molecular Sciences grapevine <i>VyTRXy</i> drought photosynthesis |
| title | Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants |
| title_full | Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants |
| title_fullStr | Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants |
| title_full_unstemmed | Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants |
| title_short | Overexpression of Grapevine <i>VyTRXy</i> Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Plants |
| title_sort | overexpression of grapevine i vytrxy i improves drought tolerance by maintaining photosynthesis and enhancing the antioxidant and osmolyte capacity of plants |
| topic | grapevine <i>VyTRXy</i> drought photosynthesis |
| url | https://www.mdpi.com/1422-0067/24/22/16388 |
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