Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i>
Background: Previously published work has demonstrated that the LPS injection of <i>Ciona robusta</i> leads to the overexpression of a truncated form of an immune-related mRNA (C8short) by means of <i>Ciona robusta</i> (CR) alternative polyadenylation (APA) (CR-APA). Methods:...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/18/4/209 |
| _version_ | 1827718809650200576 |
|---|---|
| author | Valeria Longo Alessandra Longo Annamaria Martorana Antonino Lauria Giuseppa Augello Antonina Azzolina Melchiorre Cervello Paolo Colombo |
| author_facet | Valeria Longo Alessandra Longo Annamaria Martorana Antonino Lauria Giuseppa Augello Antonina Azzolina Melchiorre Cervello Paolo Colombo |
| author_sort | Valeria Longo |
| collection | DOAJ |
| description | Background: Previously published work has demonstrated that the LPS injection of <i>Ciona robusta</i> leads to the overexpression of a truncated form of an immune-related mRNA (C8short) by means of <i>Ciona robusta</i> (CR) alternative polyadenylation (APA) (CR-APA). Methods: The 3D structure of the C8short-derived <i>Ciona robusta</i> chemo-attractive peptide (CrCP) was evaluated by homology modeling. The biological activity of the CrCP was studied in vitro using a primary human dermal cell line (HuDe). Real-Time PCR was used to investigate the expression levels of genes involved in cell motility. NF-κB signaling was studied by western blotting. Results: In silico modeling showed that CrCP displayed structural characteristics already reported for a short domain of the vertebrate CRK gene, suggesting its possible involvement in cell migration mechanisms. In vitro assays demonstrated that CrCP was capable of inducing the motility of HuDe cells in both wound healing and chemo-attractive experiments. qPCR demonstrated the capability of CrCP to modulate the expression of the matrix metalloproteinase-7 (MMP-7) and E-cadherin genes. Finally, western blot analysis demonstrated that treatment with CrCP induced activation of the NF-κB signaling pathway. Conclusion: Our results describe the characterization of the 3D structure and chemo-attractive activity of an LPS-induced CrCP peptide from <i>Ciona robusta</i>. |
| first_indexed | 2024-03-10T20:31:09Z |
| format | Article |
| id | doaj.art-92d8046f77474f89a83b7515b207de77 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T20:31:09Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-92d8046f77474f89a83b7515b207de772023-11-19T21:25:08ZengMDPI AGMarine Drugs1660-33972020-04-0118420910.3390/md18040209Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i>Valeria Longo0Alessandra Longo1Annamaria Martorana2Antonino Lauria3Giuseppa Augello4Antonina Azzolina5Melchiorre Cervello6Paolo Colombo7Institute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyDipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche “STEBICEF”, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche “STEBICEF”, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, ItalyBackground: Previously published work has demonstrated that the LPS injection of <i>Ciona robusta</i> leads to the overexpression of a truncated form of an immune-related mRNA (C8short) by means of <i>Ciona robusta</i> (CR) alternative polyadenylation (APA) (CR-APA). Methods: The 3D structure of the C8short-derived <i>Ciona robusta</i> chemo-attractive peptide (CrCP) was evaluated by homology modeling. The biological activity of the CrCP was studied in vitro using a primary human dermal cell line (HuDe). Real-Time PCR was used to investigate the expression levels of genes involved in cell motility. NF-κB signaling was studied by western blotting. Results: In silico modeling showed that CrCP displayed structural characteristics already reported for a short domain of the vertebrate CRK gene, suggesting its possible involvement in cell migration mechanisms. In vitro assays demonstrated that CrCP was capable of inducing the motility of HuDe cells in both wound healing and chemo-attractive experiments. qPCR demonstrated the capability of CrCP to modulate the expression of the matrix metalloproteinase-7 (MMP-7) and E-cadherin genes. Finally, western blot analysis demonstrated that treatment with CrCP induced activation of the NF-κB signaling pathway. Conclusion: Our results describe the characterization of the 3D structure and chemo-attractive activity of an LPS-induced CrCP peptide from <i>Ciona robusta</i>.https://www.mdpi.com/1660-3397/18/4/209<i>Ciona robusta</i>inflammationchemoattractive peptideNF-kB3D modelling |
| spellingShingle | Valeria Longo Alessandra Longo Annamaria Martorana Antonino Lauria Giuseppa Augello Antonina Azzolina Melchiorre Cervello Paolo Colombo Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> Marine Drugs <i>Ciona robusta</i> inflammation chemoattractive peptide NF-kB 3D modelling |
| title | Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> |
| title_full | Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> |
| title_fullStr | Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> |
| title_full_unstemmed | Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> |
| title_short | Identification of an LPS-Induced Chemo-Attractive Peptide from <i>Ciona robusta</i> |
| title_sort | identification of an lps induced chemo attractive peptide from i ciona robusta i |
| topic | <i>Ciona robusta</i> inflammation chemoattractive peptide NF-kB 3D modelling |
| url | https://www.mdpi.com/1660-3397/18/4/209 |
| work_keys_str_mv | AT valerialongo identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT alessandralongo identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT annamariamartorana identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT antoninolauria identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT giuseppaaugello identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT antoninaazzolina identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT melchiorrecervello identificationofanlpsinducedchemoattractivepeptidefromicionarobustai AT paolocolombo identificationofanlpsinducedchemoattractivepeptidefromicionarobustai |