Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria
<i>Staphylococcus aureus</i> is an opportunistic bacterium causing a wide variety of diseases. Biofilm formation of <i>Staphylococcus aureus</i> is of primary public and animal health concern. The purposes of the present study were to investigate the ability of <i>Staph...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/2/153 |
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| author | Rachid Achek Helmut Hotzel Ibrahim Nabi Souad Kechida Djamila Mami Nassima Didouh Herbert Tomaso Heinrich Neubauer Ralf Ehricht Stefan Monecke Hosny El-Adawy |
| author_facet | Rachid Achek Helmut Hotzel Ibrahim Nabi Souad Kechida Djamila Mami Nassima Didouh Herbert Tomaso Heinrich Neubauer Ralf Ehricht Stefan Monecke Hosny El-Adawy |
| author_sort | Rachid Achek |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is an opportunistic bacterium causing a wide variety of diseases. Biofilm formation of <i>Staphylococcus aureus</i> is of primary public and animal health concern. The purposes of the present study were to investigate the ability of <i>Staphylococcus aureus</i> isolated from animals, humans, and food samples to form biofilms and to screen for the presence of biofilm-associated and regulatory genes. In total, 55 <i>Staphylococcus aureus</i> isolated from sheep mastitis cases (<i>n</i> = 28), humans (<i>n</i> = 19), and from food matrices (<i>n</i> = 8) were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The ability of <i>Staphylococcus aureus</i> for slime production and biofilm formation was determined quantitatively. A DNA microarray examination was performed to detect adhesion genes (<i>ica</i>ACD and biofilm-associated protein gene (<i>bap</i>)), genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), regulatory genes (accessory gene regulator (<i>agr</i>) and staphylococcal accessory regulator (<i>sar</i>A)), and the staphylococcal cassette chromosome <i>mec</i> elements (SCC<i>mec</i>). Out of 55 <i>Staphylococcus aureus</i> isolates, 39 (71.0%) and 23 (41.8%) were producing slime and biofilm, respectively. All <i>Staphylococcus aureus</i> strains isolated from food showed biofilm formation ability. 52.6% of the <i>Staphylococcus aureus</i> strains isolated from sheep with mastitis, and 17.9% of isolates from humans, were able to form a biofilm. Microarray analysis typed the <i>Staphylococcus aureus</i> into 15 clonal complexes. Among all <i>Staphylococcus aureus</i> isolates, four of the human isolates (21.1%) harbored the <i>mec</i>A gene (SCC<i>mec</i> type IV) typed into 2 clonal complexes (CC22-MRSA-IV and CC80-MRSA-IV) and were considered as methicillin-resistant, while two of them were slime-producing. None of the isolates from sheep with mastitis harbored the <i>cna</i> gene which is associated with biofilm production. The <i>fnb</i>B gene was found in 100%, 60% and 40% of biofilm-producing <i>Staphylococcus aureus</i> isolated from food, humans, and sheep with mastitis, respectively. Three <i>agr</i> groups were present and <i>agr</i> group III was predominant with 43.6%, followed by <i>agr</i> group I (38.2%), and <i>agr</i> group II (18.2%). This study revealed the capacity of <i>Staphylococcus aureus</i> isolates to form biofilms and highlighted the genetic background displayed by <i>Staphylococcus aureus</i> isolates from different sources in Algeria. |
| first_indexed | 2024-04-14T03:29:15Z |
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| language | English |
| last_indexed | 2024-04-14T03:29:15Z |
| publishDate | 2020-02-01 |
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| series | Pathogens |
| spelling | doaj.art-92daf207693244818a938c59392e2b5c2022-12-22T02:15:02ZengMDPI AGPathogens2076-08172020-02-019215310.3390/pathogens9020153pathogens9020153Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in AlgeriaRachid Achek0Helmut Hotzel1Ibrahim Nabi2Souad Kechida3Djamila Mami4Nassima Didouh5Herbert Tomaso6Heinrich Neubauer7Ralf Ehricht8Stefan Monecke9Hosny El-Adawy10Faculty of Nature and Life and Earth Sciences, Djilali-Bounaama University, Soufay, Khemis-Miliana 44225, AlgeriaInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, GermanyFaculty of Sciences, Yahia-Farès University, Urban Pole, 26000 Médéa, AlgeriaFaculty of Sciences, Yahia-Farès University, Urban Pole, 26000 Médéa, AlgeriaFaculty of Sciences, Yahia-Farès University, Urban Pole, 26000 Médéa, AlgeriaFaculty of Nature and Life and Earth Sciences, Djilali-Bounaama University, Soufay, Khemis-Miliana 44225, AlgeriaInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, GermanyInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany<i>Staphylococcus aureus</i> is an opportunistic bacterium causing a wide variety of diseases. Biofilm formation of <i>Staphylococcus aureus</i> is of primary public and animal health concern. The purposes of the present study were to investigate the ability of <i>Staphylococcus aureus</i> isolated from animals, humans, and food samples to form biofilms and to screen for the presence of biofilm-associated and regulatory genes. In total, 55 <i>Staphylococcus aureus</i> isolated from sheep mastitis cases (<i>n</i> = 28), humans (<i>n</i> = 19), and from food matrices (<i>n</i> = 8) were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The ability of <i>Staphylococcus aureus</i> for slime production and biofilm formation was determined quantitatively. A DNA microarray examination was performed to detect adhesion genes (<i>ica</i>ACD and biofilm-associated protein gene (<i>bap</i>)), genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), regulatory genes (accessory gene regulator (<i>agr</i>) and staphylococcal accessory regulator (<i>sar</i>A)), and the staphylococcal cassette chromosome <i>mec</i> elements (SCC<i>mec</i>). Out of 55 <i>Staphylococcus aureus</i> isolates, 39 (71.0%) and 23 (41.8%) were producing slime and biofilm, respectively. All <i>Staphylococcus aureus</i> strains isolated from food showed biofilm formation ability. 52.6% of the <i>Staphylococcus aureus</i> strains isolated from sheep with mastitis, and 17.9% of isolates from humans, were able to form a biofilm. Microarray analysis typed the <i>Staphylococcus aureus</i> into 15 clonal complexes. Among all <i>Staphylococcus aureus</i> isolates, four of the human isolates (21.1%) harbored the <i>mec</i>A gene (SCC<i>mec</i> type IV) typed into 2 clonal complexes (CC22-MRSA-IV and CC80-MRSA-IV) and were considered as methicillin-resistant, while two of them were slime-producing. None of the isolates from sheep with mastitis harbored the <i>cna</i> gene which is associated with biofilm production. The <i>fnb</i>B gene was found in 100%, 60% and 40% of biofilm-producing <i>Staphylococcus aureus</i> isolated from food, humans, and sheep with mastitis, respectively. Three <i>agr</i> groups were present and <i>agr</i> group III was predominant with 43.6%, followed by <i>agr</i> group I (38.2%), and <i>agr</i> group II (18.2%). This study revealed the capacity of <i>Staphylococcus aureus</i> isolates to form biofilms and highlighted the genetic background displayed by <i>Staphylococcus aureus</i> isolates from different sources in Algeria.https://www.mdpi.com/2076-0817/9/2/153<i>staphylococcus aureus</i>biofilm formationadhesion genesmicroarray assayalgeria |
| spellingShingle | Rachid Achek Helmut Hotzel Ibrahim Nabi Souad Kechida Djamila Mami Nassima Didouh Herbert Tomaso Heinrich Neubauer Ralf Ehricht Stefan Monecke Hosny El-Adawy Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria Pathogens <i>staphylococcus aureus</i> biofilm formation adhesion genes microarray assay algeria |
| title | Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria |
| title_full | Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria |
| title_fullStr | Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria |
| title_full_unstemmed | Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria |
| title_short | Phenotypic and Molecular Detection of Biofilm Formation in <i>Staphylococcus aureus</i> Isolated from Different Sources in Algeria |
| title_sort | phenotypic and molecular detection of biofilm formation in i staphylococcus aureus i isolated from different sources in algeria |
| topic | <i>staphylococcus aureus</i> biofilm formation adhesion genes microarray assay algeria |
| url | https://www.mdpi.com/2076-0817/9/2/153 |
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