Oriented Immobilization of His-Tagged Protein on a Redox Active Thiol Derivative of DPTA-Cu(II) Layer Deposited on a Gold Electrode—The Base of Electrochemical Biosensors

Oriented Immobilization of His-Tagged Protein on a Redox Active Thiol Derivative of DPTA-Cu(II) Layer Deposited on a Gold Electrode—The Base of Electrochemical Biosensors

This paper concerns the development of an electrochemical biosensor for the determination of Aβ16–23' and Aβ1–40 peptides. The His-tagged V and VC1 domains of Receptor for Advanced Glycation end Products (RAGE) immobilized on a gold electrode surface were used as analytically active molecul...

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Bibliographic Details
Main Authors: Hanna Radecka, Jerzy Radecki, Wim Dehaen, Peter Verwilst, Ilona Marszałek, Aleksandra Wysłouch-Cieszyńska, Magdalena Sulima, Edyta Mikuła
Format: Article
Language:English
Published: MDPI AG 2013-09-01
Series:Sensors
Subjects:
pentetic acid–Cu(II) complex
gold electrodes
His-tagged proteins
Aβ peptides
electrochemical biosensor
Online Access:http://www.mdpi.com/1424-8220/13/9/11586

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