Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa.
<h4>Purpose</h4>To investigate whether raised levels of retinal cyclic guanosine monophosphate (cGMP) was reflected in plasma levels in PDE6A-/- dogs.<h4>Materials and methods</h4>Retina was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 6), heterozygous dogs (PDE6A+...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0279437 |
| _version_ | 1797961218084831232 |
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| author | Laurence M Occelli Kelian Sun Paige A Winkler Brandy J Morgan Simon M Petersen-Jones |
| author_facet | Laurence M Occelli Kelian Sun Paige A Winkler Brandy J Morgan Simon M Petersen-Jones |
| author_sort | Laurence M Occelli |
| collection | DOAJ |
| description | <h4>Purpose</h4>To investigate whether raised levels of retinal cyclic guanosine monophosphate (cGMP) was reflected in plasma levels in PDE6A-/- dogs.<h4>Materials and methods</h4>Retina was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 6), heterozygous dogs (PDE6A+/-, N = 4) and affected dogs (PDE6A-/-, N = 3) and plasma was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 5), heterozygous dogs (PDE6A+/-, N = 5) and affected dogs (PDE6A-/-, N = 5). Retina and plasma samples were measured by ELISA.<h4>Results</h4>cGMP levels in retinal samples of PDE6A-/- dogs at 2 months of age were significantly elevated. There was no significant difference in plasma cGMP levels between wildtype and PDE6A-/- or PDE6A+/- puppies. However, the plasma cGMP levels of the PDE6A-/- puppies were significantly lower than that of PDE6A+/- puppies.<h4>Conclusion</h4>cGMP levels in the plasma from PDE6A-/- was not elevated when compared to control dogs. At the 2-month timepoint, cGMP plasma levels would not be a useful biomarker for disease. |
| first_indexed | 2024-04-11T00:55:37Z |
| format | Article |
| id | doaj.art-92ebe64fc9854b2dbbca7218a2b3eae3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T00:55:37Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-92ebe64fc9854b2dbbca7218a2b3eae32023-01-05T05:31:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712e027943710.1371/journal.pone.0279437Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa.Laurence M OccelliKelian SunPaige A WinklerBrandy J MorganSimon M Petersen-Jones<h4>Purpose</h4>To investigate whether raised levels of retinal cyclic guanosine monophosphate (cGMP) was reflected in plasma levels in PDE6A-/- dogs.<h4>Materials and methods</h4>Retina was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 6), heterozygous dogs (PDE6A+/-, N = 4) and affected dogs (PDE6A-/-, N = 3) and plasma was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 5), heterozygous dogs (PDE6A+/-, N = 5) and affected dogs (PDE6A-/-, N = 5). Retina and plasma samples were measured by ELISA.<h4>Results</h4>cGMP levels in retinal samples of PDE6A-/- dogs at 2 months of age were significantly elevated. There was no significant difference in plasma cGMP levels between wildtype and PDE6A-/- or PDE6A+/- puppies. However, the plasma cGMP levels of the PDE6A-/- puppies were significantly lower than that of PDE6A+/- puppies.<h4>Conclusion</h4>cGMP levels in the plasma from PDE6A-/- was not elevated when compared to control dogs. At the 2-month timepoint, cGMP plasma levels would not be a useful biomarker for disease.https://doi.org/10.1371/journal.pone.0279437 |
| spellingShingle | Laurence M Occelli Kelian Sun Paige A Winkler Brandy J Morgan Simon M Petersen-Jones Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. PLoS ONE |
| title | Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. |
| title_full | Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. |
| title_fullStr | Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. |
| title_full_unstemmed | Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. |
| title_short | Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa. |
| title_sort | elevated retinal cgmp is not associated with elevated circulating cgmp levels in a canine model of retinitis pigmentosa |
| url | https://doi.org/10.1371/journal.pone.0279437 |
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