Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways
Dietary protein supplementation has emerged as a promising strategy in combating sarcopenia. Furthermore, searching for alternatives of animal proteins has been a hot topic. The present study aimed to investigate the effects of zein peptides on C<sub>2</sub>C<sub>12</sub> myo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/13/6/919 |
| _version_ | 1797241048423989248 |
|---|---|
| author | Mohammad Sadiq Amin Binbin Yu Dongjing Wu Yujia Lu Wei Wu Jing Wang Yuhao Zhang Yu Fu |
| author_facet | Mohammad Sadiq Amin Binbin Yu Dongjing Wu Yujia Lu Wei Wu Jing Wang Yuhao Zhang Yu Fu |
| author_sort | Mohammad Sadiq Amin |
| collection | DOAJ |
| description | Dietary protein supplementation has emerged as a promising strategy in combating sarcopenia. Furthermore, searching for alternatives of animal proteins has been a hot topic. The present study aimed to investigate the effects of zein peptides on C<sub>2</sub>C<sub>12</sub> myoblasts and explore their potential molecular mechanisms. The proliferative, cell cycle, and anti-apoptotic activities of zein peptides were evaluated. Peptidomics analysis and transcriptome sequencing were employed to explore the structure-activity relationship and underlying molecular mechanisms. The results indicated that zein peptides (0.05–0.2 mg/mL) exerted a significant proliferation-promoting impact on C<sub>2</sub>C<sub>12</sub> cells, via increasing cell viability by 33.37 to 42.39%. Furthermore, zein peptides significantly increased S phase proportion and decreased the apoptosis rate from 34.08% (model group) to 28.96% in C<sub>2</sub>C<sub>12</sub> cells. In addition, zein peptides exhibited a pronounced anti-apoptotic effect on C<sub>2</sub>C<sub>12</sub> cells. Zein peptides are abundant in branch-chain amino acids, especially leucine. Transcriptomics analysis revealed that zein peptides can promote proliferation, accelerate cell cycle, and improve protein synthesis of muscle cells through mTORC1 and mTORC2 signaling pathways. |
| first_indexed | 2024-04-24T18:17:08Z |
| format | Article |
| id | doaj.art-92ebf876b5e24bfbafb3a6a1007511db |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-24T18:17:08Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-92ebf876b5e24bfbafb3a6a1007511db2024-03-27T13:41:10ZengMDPI AGFoods2304-81582024-03-0113691910.3390/foods13060919Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling PathwaysMohammad Sadiq Amin0Binbin Yu1Dongjing Wu2Yujia Lu3Wei Wu4Jing Wang5Yuhao Zhang6Yu Fu7College of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaDepartment of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USACollege of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaChina-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University (BTBU), Beijing 100048, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaDietary protein supplementation has emerged as a promising strategy in combating sarcopenia. Furthermore, searching for alternatives of animal proteins has been a hot topic. The present study aimed to investigate the effects of zein peptides on C<sub>2</sub>C<sub>12</sub> myoblasts and explore their potential molecular mechanisms. The proliferative, cell cycle, and anti-apoptotic activities of zein peptides were evaluated. Peptidomics analysis and transcriptome sequencing were employed to explore the structure-activity relationship and underlying molecular mechanisms. The results indicated that zein peptides (0.05–0.2 mg/mL) exerted a significant proliferation-promoting impact on C<sub>2</sub>C<sub>12</sub> cells, via increasing cell viability by 33.37 to 42.39%. Furthermore, zein peptides significantly increased S phase proportion and decreased the apoptosis rate from 34.08% (model group) to 28.96% in C<sub>2</sub>C<sub>12</sub> cells. In addition, zein peptides exhibited a pronounced anti-apoptotic effect on C<sub>2</sub>C<sub>12</sub> cells. Zein peptides are abundant in branch-chain amino acids, especially leucine. Transcriptomics analysis revealed that zein peptides can promote proliferation, accelerate cell cycle, and improve protein synthesis of muscle cells through mTORC1 and mTORC2 signaling pathways.https://www.mdpi.com/2304-8158/13/6/919myoblastzein peptidescell proliferationcell cycleanti-apoptoticmolecular mechanism |
| spellingShingle | Mohammad Sadiq Amin Binbin Yu Dongjing Wu Yujia Lu Wei Wu Jing Wang Yuhao Zhang Yu Fu Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways Foods myoblast zein peptides cell proliferation cell cycle anti-apoptotic molecular mechanism |
| title | Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways |
| title_full | Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways |
| title_fullStr | Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways |
| title_full_unstemmed | Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways |
| title_short | Zein-Derived Peptides from Corn Promote the Proliferation of C<sub>2</sub>C<sub>12</sub> Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways |
| title_sort | zein derived peptides from corn promote the proliferation of c sub 2 sub c sub 12 sub myoblasts via crosstalk of mtorc1 and mtorc2 signaling pathways |
| topic | myoblast zein peptides cell proliferation cell cycle anti-apoptotic molecular mechanism |
| url | https://www.mdpi.com/2304-8158/13/6/919 |
| work_keys_str_mv | AT mohammadsadiqamin zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT binbinyu zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT dongjingwu zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT yujialu zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT weiwu zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT jingwang zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT yuhaozhang zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways AT yufu zeinderivedpeptidesfromcornpromotetheproliferationofcsub2subcsub12submyoblastsviacrosstalkofmtorc1andmtorc2signalingpathways |