A Novel qPCR Method for the Detection of Lactic Acid Bacteria in Fermented Milk

A Novel qPCR Method for the Detection of Lactic Acid Bacteria in Fermented Milk

The number of live lactic acid bacteria (LAB) is an important quality indicator for yogurt, the quantitative testing of LAB has become an important task in the evaluation of product quality and function. By analyzing and comparing the performance of 16S rRNA gene and <i>tuf</i> gene used...

Full description

Bibliographic Details
Main Authors:	Xiankang Fan, Xiefei Li, Tao Zhang, Jue Xu, Zihang Shi, Zhen Wu, Jihuan Wu, Daodong Pan, Lihui Du
Format:	Article
Language:	English
Published:	MDPI AG 2021-12-01
Series:	Foods
Subjects:	qPCR lactic acid bacteria yogurt laser confocal microscope <i>tuf</i> gene flow cytometry
Online Access:	https://www.mdpi.com/2304-8158/10/12/3066

Similar Items

PMA-qPCR method for the selective quantitation of viable lactic acid bacteria in fermented milk
by: Zihang Shi, et al.
Published: (2022-09-01)

Characterization of a novel flavored yogurt enriched in γ-aminobutyric acid fermented by Levilactobacillus brevis CGMCC1.5954
by: Xiankang Fan, et al.
Published: (2023-02-01)

New Genetic Determinants for qPCR Identification and the Enumeration of Selected Lactic Acid Bacteria in Raw-Milk Cheese
by: Milena Alicja Stachelska, et al.
Published: (2024-03-01)

Establishment of a Rapid LAMP Assay for <i>Aeromonas hydrophila</i> and Comparison with the Application of qPCR
by: Zihui Gao, et al.
Published: (2023-07-01)

Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the <i>Gardnerella</i>-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis
by: Agnieszka Latka, et al.
Published: (2022-01-01)

Effects of Semen Ziziphi Spinosae extract and binary probiotics co-fermentation on the quality of yogurt and their underlying molecular mechanisms
by: Xiankang Fan, et al.
Published: (2024-03-01)

Evaluation of Reference Genes in the Polyploid Complex <i>Dianthus broteri</i> (Caryophyllaceae) Using qPCR
by: Alba Rodríguez-Parra, et al.
Published: (2022-02-01)

Constructing an In Vitro and In Vivo Flow Cytometry by Fast Line Scanning of Confocal Microscopy
by: Xiaohui Zhao, et al.
Published: (2023-03-01)

Development of a Multiplex qPCR Assay for Fast Detection and Differentiation of Paracoccidioidomycosis Agents
by: Breno Gonçalves Pinheiro, et al.
Published: (2023-03-01)

A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water
by: Yong Shao, et al.
Published: (2023-01-01)

Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of <i>Bacteroides fragilis</i>
by: Bakhtiyar Mahmood, et al.
Published: (2024-02-01)

Taqman qPCR Quantification and <i>Fusarium</i> Community Analysis to Evaluate Toxigenic Fungi in Cereals
by: Elina Sohlberg, et al.
Published: (2022-01-01)

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR
by: Fatemeh Nejati, et al.
Published: (2020-06-01)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
by: Baowei Li, et al.
Published: (2021-11-01)

Selection and Evaluation of Reference Genes for RT-qPCR Analysis in <i>Amorphophallus</i> Konjac Based on Transcriptome Data
by: Yanli Liu, et al.
Published: (2023-07-01)

Laboratory Exercise to Measure Plasmid Copy Number by qPCR
by: Benjamin David, et al.
Published: (2021-09-01)

Development of PCR, LAMP and qPCR Assays for the Detection of Aflatoxigenic Strains of <i>Aspergillus</i> <i>flavus</i> and <i>A. parasiticus</i> in Hazelnut
by: Sara Franco Ortega, et al.
Published: (2020-11-01)

Simplifying Genotyping of Mutants from Genome Editing with a Parallel qPCR-Based iGenotype Index
by: Liezhen Fu, et al.
Published: (2024-01-01)

Comparison of Two Commercially Available qPCR Kits for the Detection of <i>Candida auris</i>
by: Janko Sattler, et al.
Published: (2021-02-01)

Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future
by: Meagan Archer, et al.
Published: (2021-06-01)

Characterisation and probiotic potential of lactic acid bacteria isolated from Iranian traditional yogurts
by: Mohammad Kazem Sharifi Yazdi, et al.
Published: (2017-04-01)

Moving from qPCR to Chip Digital PCR Assays for Tracking of some <i>Fusarium</i> Species Causing <i>Fusarium</i> Head Blight in Cereals
by: Caterina Morcia, et al.
Published: (2020-08-01)

Authentication of caprine milk and cheese by commercial qPCR assay
by: Tomáš Fekete, et al.
Published: (2017-01-01)

Evaluation of Quantitative PCR (qPCR) <i>Paenibacillus larvae</i> Targeted Assays and Definition of Optimal Conditions for Its Detection/Quantification in Honey and Hive Debris
by: Franca Rossi, et al.
Published: (2018-11-01)

Persistence of <i>Legionella</i> in Routinely Disinfected Heater-Cooler Units and Heater Units assessed by Propidium Monoazide qPCR
by: Savina Ditommaso, et al.
Published: (2020-11-01)

Fermentation and Quality Characteristics of Yogurt Treated with <i>Bifidobacterium longum</i>
by: Jang Keun Son, et al.
Published: (2023-08-01)

Evaluation of a kDNA-Based qPCR Assay for the Detection and Quantification of Old World <i>Leishmania</i> Species
by: Marcello Ceccarelli, et al.
Published: (2020-12-01)

Importance of Adequate qPCR Controls in Infection Control
by: Matthew Oughton, et al.
Published: (2021-12-01)

Reference Genes for Expression Analysis Using RT-qPCR in <i>Cnaphalocrocis medinalis</i> (Lepidoptera: Pyralidae)
by: Xiaoyu Zhao, et al.
Published: (2022-11-01)

Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility
by: Inés Arrieta-Aguirre, et al.
Published: (2023-11-01)

A Quadruplex qPCR for Detection and Differentiation of Classic and Natural Recombinant Myxoma Virus Strains of Leporids
by: Fábio A. Abade dos Santos, et al.
Published: (2021-11-01)

Stable Reference Genes for qPCR Analysis in BM-MSCs Undergoing Osteogenic Differentiation within 3D Hyaluronan-Based Hydrogels
by: Johannes Hasler, et al.
Published: (2020-12-01)

Sequence Analysis of New <i>Tuf</i> Molecular Types of ‘<i>Candidatus</i> Phytoplasma Solani’ in Iranian Vineyards
by: Elham Jamshidi, et al.
Published: (2020-06-01)

Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR
by: Hyoung-Rai Ko, et al.
Published: (2019-12-01)

Identiﬁ cation of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR
by: Rublenko N.
Published: (2020-05-01)

Design and Validation of qPCR-Specific Primers for Quantification of the Marketed <i>Terfezia claveryi</i> and <i>Terfezia crassiverrucosa</i> in Soil
by: Francisco Arenas, et al.
Published: (2022-10-01)

Investigating Useful Properties of Four <i>Streptomyces</i> Strains Active against <i>Fusarium graminearum</i> Growth and Deoxynivalenol Production on Wheat Grains by qPCR
by: Elena Maria Colombo, et al.
Published: (2020-08-01)

Development of a Method for Detecting and Estimating <i>Moniliophthora roreri</i> Spore Loads Based on Spore Traps and qPCR
by: Diana L. Jiménez-Zapata, et al.
Published: (2022-12-01)

qPCRtools: An R package for qPCR data processing and visualization
by: Xiang Li, et al.
Published: (2022-09-01)

DNA vs RNA based studies of nitrogen removal bacteria genes via qPCR
by: Anna Banach-Wiśniewska, et al.
Published: (2021-03-01)