Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training
ABSTRACT Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2019-01-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-1149-en.html |
| _version_ | 1819078033085038592 |
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| author | Nasrin Ramezani Behnaz Vanaky Nader Shakeri Zahra Soltanian Fatemeh Fakhari Rad Zahra Shams |
| author_facet | Nasrin Ramezani Behnaz Vanaky Nader Shakeri Zahra Soltanian Fatemeh Fakhari Rad Zahra Shams |
| author_sort | Nasrin Ramezani |
| collection | DOAJ |
| description | ABSTRACT
Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats.
Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant.
Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention.
Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes.
Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes. |
| first_indexed | 2024-12-21T19:06:39Z |
| format | Article |
| id | doaj.art-92efea375a2a410f838b3d05ca51a7bd |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-21T19:06:39Z |
| publishDate | 2019-01-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-92efea375a2a410f838b3d05ca51a7bd2022-12-21T18:53:19ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492019-01-011311520Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval TrainingNasrin Ramezani0Behnaz Vanaky1Nader Shakeri2Zahra Soltanian3Fatemeh Fakhari Rad4Zahra Shams5 Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Tehran University, Tehran, Iran Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran Department of Sport Physiology, Faculty of Physical Education and Sports science, Central Tehran Branch, Islamic Azad University, Tehran, Iran ABSTRACT Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats. Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant. Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention. Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes. Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes.http://mlj.goums.ac.ir/article-1-1149-en.htmlbaxbcl-2high intensity interval traininghearttype 2 diabetes. |
| spellingShingle | Nasrin Ramezani Behnaz Vanaky Nader Shakeri Zahra Soltanian Fatemeh Fakhari Rad Zahra Shams Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training Medical Laboratory Journal bax bcl-2 high intensity interval training heart type 2 diabetes. |
| title | Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training |
| title_full | Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training |
| title_fullStr | Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training |
| title_full_unstemmed | Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training |
| title_short | Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training |
| title_sort | evaluation of bcl 2 and bax expression in the heart of diabetic rats after four weeks of high intensity interval training |
| topic | bax bcl-2 high intensity interval training heart type 2 diabetes. |
| url | http://mlj.goums.ac.ir/article-1-1149-en.html |
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