Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers
Abstract Background The search of useful serum biomarkers for the early detection of cervical cancers has been of a high priority. The activation of Macrophage-Colony Stimulating Factor (M-CSF) and Vascular Endothelial Growth Factor (VEGF) is likely involved in the pathogenesis and spread of cancer....
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-04-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-019-5558-8 |
| _version_ | 1818109325007126528 |
|---|---|
| author | Iwona Sidorkiewicz Monika Zbucka-Krętowska Kamil Zaręba Emilia Lubowicka Monika Zajkowska Maciej Szmitkowski Ewa Gacuta Sławomir Ławicki |
| author_facet | Iwona Sidorkiewicz Monika Zbucka-Krętowska Kamil Zaręba Emilia Lubowicka Monika Zajkowska Maciej Szmitkowski Ewa Gacuta Sławomir Ławicki |
| author_sort | Iwona Sidorkiewicz |
| collection | DOAJ |
| description | Abstract Background The search of useful serum biomarkers for the early detection of cervical cancers has been of a high priority. The activation of Macrophage-Colony Stimulating Factor (M-CSF) and Vascular Endothelial Growth Factor (VEGF) is likely involved in the pathogenesis and spread of cancer. We compared the plasma levels of M-CSF and VEGF to the ones of commonly accepted tumor markers CA 125and SCC-Ag in three groups of patients: 1. the cervical cancer group (patients with either squamous cell carcinoma or adenocarcinoma); 2. the cervical dysplasia group; 3. the control group. Methods This cohort study included 100 patients with cervical cancer and 55 patients with cervical dysplasia. The control group consisted of 50 healthy volunteers. The plasma levels of VEGF and M-CSF were determined using ELISA, while CA 125 and SCC-Ag concentrations were obtained by the chemiluminescent microparticle immunoassay (CMIA). Results The median levels of M-CSF and VEGF as well as CA 125 and SCC-Ag in the entire group of cervical cancer patients, were significantly different compared to the healthy women group. In case of both the squamous cell carcinoma and the adenocarcinoma groups, plasma levels of M-CSF and VEGF were higher compared to the control group. No significant differences in the studied parameters between the squamous cell carcinoma and the adenocarcinoma group were observed. The highest sensitivity and specificity were obtained for VEGF (81.18 and 76.00%, respectively) and SCC-Ag (81.18%; 74.00%) in the squamous cell carcinoma group and for VEGF (86.67%; 76.00%) in the adenocarcinoma group. The area under the ROC curve for VEGF was the largest in the adenocarcinoma group followed by the squamous cell carcinoma group (0.9082 and 0.8566 respectively). Conclusions Obtained results indicate a possible clinical applicability and a high diagnostic power for the combination of MSC-F, VEGF, CA 125 and SCC-Ag in the diagnosis of both studied types of cervical cancer. |
| first_indexed | 2024-12-11T02:29:27Z |
| format | Article |
| id | doaj.art-92f0e614c2f5419bbd0602f303aeaea5 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T02:29:27Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-92f0e614c2f5419bbd0602f303aeaea52022-12-22T01:23:52ZengBMCBMC Cancer1471-24072019-04-0119111210.1186/s12885-019-5558-8Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancersIwona Sidorkiewicz0Monika Zbucka-Krętowska1Kamil Zaręba2Emilia Lubowicka3Monika Zajkowska4Maciej Szmitkowski5Ewa Gacuta6Sławomir Ławicki7Department of Reproduction and Gynecological Endocrinology, Medical University of BialystokDepartment of Reproduction and Gynecological Endocrinology, Medical University of BialystokDepartment of Gynecology and Gynecologic Oncology, Medical University of BiałystokDepartment of Esthetic Medicine, Medical University of BialystokDepartment of Biochemical Diagnostics, Medical University of BialystokDepartment of Biochemical Diagnostics, Medical University of BialystokDepartment of Perinatology, Medical University of BialystokDepartment of Population Medicine and Civilization Diseases Prevention, Medical University of BialystokAbstract Background The search of useful serum biomarkers for the early detection of cervical cancers has been of a high priority. The activation of Macrophage-Colony Stimulating Factor (M-CSF) and Vascular Endothelial Growth Factor (VEGF) is likely involved in the pathogenesis and spread of cancer. We compared the plasma levels of M-CSF and VEGF to the ones of commonly accepted tumor markers CA 125and SCC-Ag in three groups of patients: 1. the cervical cancer group (patients with either squamous cell carcinoma or adenocarcinoma); 2. the cervical dysplasia group; 3. the control group. Methods This cohort study included 100 patients with cervical cancer and 55 patients with cervical dysplasia. The control group consisted of 50 healthy volunteers. The plasma levels of VEGF and M-CSF were determined using ELISA, while CA 125 and SCC-Ag concentrations were obtained by the chemiluminescent microparticle immunoassay (CMIA). Results The median levels of M-CSF and VEGF as well as CA 125 and SCC-Ag in the entire group of cervical cancer patients, were significantly different compared to the healthy women group. In case of both the squamous cell carcinoma and the adenocarcinoma groups, plasma levels of M-CSF and VEGF were higher compared to the control group. No significant differences in the studied parameters between the squamous cell carcinoma and the adenocarcinoma group were observed. The highest sensitivity and specificity were obtained for VEGF (81.18 and 76.00%, respectively) and SCC-Ag (81.18%; 74.00%) in the squamous cell carcinoma group and for VEGF (86.67%; 76.00%) in the adenocarcinoma group. The area under the ROC curve for VEGF was the largest in the adenocarcinoma group followed by the squamous cell carcinoma group (0.9082 and 0.8566 respectively). Conclusions Obtained results indicate a possible clinical applicability and a high diagnostic power for the combination of MSC-F, VEGF, CA 125 and SCC-Ag in the diagnosis of both studied types of cervical cancer.http://link.springer.com/article/10.1186/s12885-019-5558-8M-CSFVEGFCervical cancerSerum markerTumor markerSquamous cell carcinoma |
| spellingShingle | Iwona Sidorkiewicz Monika Zbucka-Krętowska Kamil Zaręba Emilia Lubowicka Monika Zajkowska Maciej Szmitkowski Ewa Gacuta Sławomir Ławicki Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers BMC Cancer M-CSF VEGF Cervical cancer Serum marker Tumor marker Squamous cell carcinoma |
| title | Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| title_full | Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| title_fullStr | Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| title_full_unstemmed | Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| title_short | Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| title_sort | plasma levels of m csf and vegf in laboratory diagnostics and differentiation of selected histological types of cervical cancers |
| topic | M-CSF VEGF Cervical cancer Serum marker Tumor marker Squamous cell carcinoma |
| url | http://link.springer.com/article/10.1186/s12885-019-5558-8 |
| work_keys_str_mv | AT iwonasidorkiewicz plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT monikazbuckakretowska plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT kamilzareba plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT emilialubowicka plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT monikazajkowska plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT maciejszmitkowski plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT ewagacuta plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers AT sławomirławicki plasmalevelsofmcsfandvegfinlaboratorydiagnosticsanddifferentiationofselectedhistologicaltypesofcervicalcancers |