Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_11206_95e5f8b7d94c3412373ce719195ec23e.pdf |
| _version_ | 1818514948185128960 |
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| author | Farideh Ranjibar Mahdi Habibi-Anbouhi Fatemeh Kazemi-Lomedasht Seyed Hamid Aghaee-Bakhtiyari Ehsan Alirahimi Mahdi Behdani |
| author_facet | Farideh Ranjibar Mahdi Habibi-Anbouhi Fatemeh Kazemi-Lomedasht Seyed Hamid Aghaee-Bakhtiyari Ehsan Alirahimi Mahdi Behdani |
| author_sort | Farideh Ranjibar |
| collection | DOAJ |
| description | Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR). Results: Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP. Conclusion: It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches. |
| first_indexed | 2024-12-11T00:22:38Z |
| format | Article |
| id | doaj.art-92f14b4fc4094b8483158d4275ae5d44 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-11T00:22:38Z |
| publishDate | 2018-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-92f14b4fc4094b8483158d4275ae5d442022-12-22T01:27:40ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-09-0121988488810.22038/ijbms.2018.26191.643211206Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobodyFarideh Ranjibar0Mahdi Habibi-Anbouhi1Fatemeh Kazemi-Lomedasht2Seyed Hamid Aghaee-Bakhtiyari3Ehsan Alirahimi4Mahdi Behdani5Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, IranNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, IranBiotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, IranBiotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Medical Biotechnology, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, IranBiotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, IranObjective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR). Results: Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP. Conclusion: It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches.http://ijbms.mums.ac.ir/article_11206_95e5f8b7d94c3412373ce719195ec23e.pdfBacteriophageNanobodyReceptor-mediated gene transferTargeted-gene deliveryVEGFR2 |
| spellingShingle | Farideh Ranjibar Mahdi Habibi-Anbouhi Fatemeh Kazemi-Lomedasht Seyed Hamid Aghaee-Bakhtiyari Ehsan Alirahimi Mahdi Behdani Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody Iranian Journal of Basic Medical Sciences Bacteriophage Nanobody Receptor-mediated gene transfer Targeted-gene delivery VEGFR2 |
| title | Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody |
| title_full | Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody |
| title_fullStr | Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody |
| title_full_unstemmed | Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody |
| title_short | Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody |
| title_sort | cell specific targeting by engineered m13 bacteriophage expressing vegfr2 nanobody |
| topic | Bacteriophage Nanobody Receptor-mediated gene transfer Targeted-gene delivery VEGFR2 |
| url | http://ijbms.mums.ac.ir/article_11206_95e5f8b7d94c3412373ce719195ec23e.pdf |
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