Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091
Based on previous research, related to detailed insight into mutualistic collaboration of microalga and its microbiome, we established an artificial plant-bacteria system of the microalga Micrasterias radians MZCH 672 and the bacterial isolate Dyadobacter sp. HH091. The bacteria, affiliated with the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006609/full |
| _version_ | 1798019502758166528 |
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| author | Yekaterina Astafyeva Marno Gurschke Wolfgang R. Streit Ines Krohn |
| author_facet | Yekaterina Astafyeva Marno Gurschke Wolfgang R. Streit Ines Krohn |
| author_sort | Yekaterina Astafyeva |
| collection | DOAJ |
| description | Based on previous research, related to detailed insight into mutualistic collaboration of microalga and its microbiome, we established an artificial plant-bacteria system of the microalga Micrasterias radians MZCH 672 and the bacterial isolate Dyadobacter sp. HH091. The bacteria, affiliated with the phylum Bacteroidota, strongly stimulated growth of the microalga when it was added to axenic algal cultures. For further advances, we studied the isolate HH091 and its interaction with the microalga M. radians using transcriptome and extensive genome analyses. The genome of HH091 contains predicted polysaccharide utilizing gene clusters co-working with the type IX secretion system (T9SS) and conceivably involved in the algae-bacteria liaison. Here, we focus on characterizing the mechanism of T9SS, implementing the attachment and invasion of microalga by Dyadobacter sp. HH091. Omics analysis exposed T9SS genes: gldK, gldL, gldM, gldN, sprA, sprE, sprF, sprT, porU and porV. Besides, gld genes not considered as the T9SS components but required for gliding motility and protein secretion (gldA, gldB, gldD, gldF, gldG, gldH, gldI, gldJ), were also identified at this analysis. A first model of T9SS apparatus of Dyadobacter was proposed in a course of this research. Using the combination of fluorescence labeling of Dyadobacter sp. HH091, we examined the bacterial colonisation and penetration into the cell wall of the algal host M. radians MZCH 672. |
| first_indexed | 2024-04-11T16:41:48Z |
| format | Article |
| id | doaj.art-92f188aa7a6a4260b14c45562142429e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T16:41:48Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-92f188aa7a6a4260b14c45562142429e2022-12-22T04:13:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10066091006609Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091Yekaterina AstafyevaMarno GurschkeWolfgang R. StreitInes KrohnBased on previous research, related to detailed insight into mutualistic collaboration of microalga and its microbiome, we established an artificial plant-bacteria system of the microalga Micrasterias radians MZCH 672 and the bacterial isolate Dyadobacter sp. HH091. The bacteria, affiliated with the phylum Bacteroidota, strongly stimulated growth of the microalga when it was added to axenic algal cultures. For further advances, we studied the isolate HH091 and its interaction with the microalga M. radians using transcriptome and extensive genome analyses. The genome of HH091 contains predicted polysaccharide utilizing gene clusters co-working with the type IX secretion system (T9SS) and conceivably involved in the algae-bacteria liaison. Here, we focus on characterizing the mechanism of T9SS, implementing the attachment and invasion of microalga by Dyadobacter sp. HH091. Omics analysis exposed T9SS genes: gldK, gldL, gldM, gldN, sprA, sprE, sprF, sprT, porU and porV. Besides, gld genes not considered as the T9SS components but required for gliding motility and protein secretion (gldA, gldB, gldD, gldF, gldG, gldH, gldI, gldJ), were also identified at this analysis. A first model of T9SS apparatus of Dyadobacter was proposed in a course of this research. Using the combination of fluorescence labeling of Dyadobacter sp. HH091, we examined the bacterial colonisation and penetration into the cell wall of the algal host M. radians MZCH 672.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006609/fullDyadobacter sp. HH091Micrasterias radiansmicroalgaebacteria interactionsynthetic early plant-bacteria systemsymbiotic relations |
| spellingShingle | Yekaterina Astafyeva Marno Gurschke Wolfgang R. Streit Ines Krohn Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 Frontiers in Microbiology Dyadobacter sp. HH091 Micrasterias radians microalgaebacteria interaction synthetic early plant-bacteria system symbiotic relations |
| title | Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 |
| title_full | Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 |
| title_fullStr | Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 |
| title_full_unstemmed | Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 |
| title_short | Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091 |
| title_sort | interplay between the microalgae micrasterias radians and its symbiont dyadobacter sp hh091 |
| topic | Dyadobacter sp. HH091 Micrasterias radians microalgaebacteria interaction synthetic early plant-bacteria system symbiotic relations |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006609/full |
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