Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle homogenate. This protocol can be easily adapted for...
| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Elsevier
2023-03-01
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| Colecção: | STAR Protocols |
| Assuntos: | |
| Acesso em linha: | http://www.sciencedirect.com/science/article/pii/S266616672200867X |