Measuring SERCA-mediated calcium uptake in mouse muscle homogenates

Measuring SERCA-mediated calcium uptake in mouse muscle homogenates

Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle homogenate. This protocol can be easily adapted for...

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Bibliographic Details
Main Authors:	Mia S. Geromella, Jessica L. Braun, Val A. Fajardo
Format:	Article
Language:	English
Published:	Elsevier 2023-03-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell-based Assays Metabolism
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672200867X

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