The decreasing of NFκB level in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with application of 1% curcumin on gingival sulcus
<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: no...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2015-03-01
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| Series: | Dental Journal |
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| Online Access: | http://e-journal.unair.ac.id/index.php/MKG/article/view/705 |
| Summary: | <p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Periodontal disease is a chronic, multi-factorial disease. Chronic periodontitis is one of the main causes of tooth </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>loss. Chronic periodontitis is usually caused by Porphyromonas gingivalis (P. gingivalis). P. gingivalis can induce NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B activation </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>resulting in the increasing of periodontal extracellular matrix degradation. Curcumin can inhibit NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B activation and reduce the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>severity of periodontal degradation. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This research was aimed to observe level of NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B in gingival junctional epithelium of </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>rat exposed to Porphyromonas gingivalis with local administration of curcumin. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sixteen Wistar rat were divided into two </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>groups. Group 1 (treatment) consisted of eight rat given 2 x 10</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>6</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>CFU/ml P. gingivalis and 1% curcumin. Meanwhile, group 2 (control) </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>consisted of eight rat given 2 x 10</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>6 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>CFU/ml P. gingivalis only. GCF samples were collected from gingival sulcus. The samples were </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>biochemically analyzed with ELISA method. Data were then analyzed statistically by using independent t-test (</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">α<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>=0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>examination of NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B level showed that there was significant difference between treatment group and control group (p<0.05). The level </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B in the treatment group was significantly lower than the control group. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>It can be concluded that 1% curcumin </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>application can reduce NF</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">κ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>B level in gingival junctional epithelium of rat exposed to P. gingivalis.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p> |
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| ISSN: | 1978-3728 2442-9740 |