Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis
The effects of the heavy metals cadmium and copper (50-500 µM) on the apoptotic events involving changes in mitochondrial membrane potential (∆Ψm) and phosphatidylserine (PS) translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent p...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2011-06-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/240 |
| _version_ | 1818560024264310784 |
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| author | F M Bearoff S L Fuller-Espie |
| author_facet | F M Bearoff S L Fuller-Espie |
| author_sort | F M Bearoff |
| collection | DOAJ |
| description | The effects of the heavy metals cadmium and copper (50-500 µM) on the apoptotic events
involving changes in mitochondrial membrane potential (∆Ψm) and phosphatidylserine (PS)
translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent probe JC-1, loss of membrane potential due to depolarization was detected in a greater proportion of cases when induced by cadmium compared to copper (58.7 % vs. 37 %) and at a lower concentration (50 µM vs. 125µM). With the use of the general caspase inhibitor Z-VAD-fmk, PS translocation detected by annexin V-FITC was found to be caspase-dependent when induced by cadmium at 125-250 µM but not at 50 µM or 500 µM; a high proportion of earthworms (60 %) exhibited inhibitory effects. Additionally, the collapse in membrane potential and PS translocation were found to strongly correlate (r > 0.5) in 89 % of cases when induced by cadmium and copper. Thus, heavy metals appear to induce death in celomocytes of E. hortensis through apoptosis by means of caspase dependent pathways. |
| first_indexed | 2024-12-14T00:33:04Z |
| format | Article |
| id | doaj.art-92fcf547f49840b6aff92d30fb5a1558 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-14T00:33:04Z |
| publishDate | 2011-06-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-92fcf547f49840b6aff92d30fb5a15582022-12-21T23:24:46ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2011-06-0181Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensisF M Bearoff0S L Fuller-Espie1Science Department, Cabrini College, 610 King of Prussia Road, Radnor, Pennsylvania 19087-3698, USAScience Department, Cabrini College, 610 King of Prussia Road, Radnor, Pennsylvania 19087-3698, USAThe effects of the heavy metals cadmium and copper (50-500 µM) on the apoptotic events involving changes in mitochondrial membrane potential (∆Ψm) and phosphatidylserine (PS) translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent probe JC-1, loss of membrane potential due to depolarization was detected in a greater proportion of cases when induced by cadmium compared to copper (58.7 % vs. 37 %) and at a lower concentration (50 µM vs. 125µM). With the use of the general caspase inhibitor Z-VAD-fmk, PS translocation detected by annexin V-FITC was found to be caspase-dependent when induced by cadmium at 125-250 µM but not at 50 µM or 500 µM; a high proportion of earthworms (60 %) exhibited inhibitory effects. Additionally, the collapse in membrane potential and PS translocation were found to strongly correlate (r > 0.5) in 89 % of cases when induced by cadmium and copper. Thus, heavy metals appear to induce death in celomocytes of E. hortensis through apoptosis by means of caspase dependent pathways.https://isj02.unimore.it/index.php/ISJ/article/view/240cadmium and copperJC-1annexin V-FITCmitochondrial membrane depolarizationphosphatidylserine translocationcaspases |
| spellingShingle | F M Bearoff S L Fuller-Espie Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis Invertebrate Survival Journal cadmium and copper JC-1 annexin V-FITC mitochondrial membrane depolarization phosphatidylserine translocation caspases |
| title | Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_full | Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_fullStr | Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_full_unstemmed | Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_short | Alteration of mitochondrial membrane potential (∆Ψm) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_sort | alteration of mitochondrial membrane potential ∆ψm and phosphatidylserine translocation as early indicators of heavy metal induced apoptosis in the earthworm eisenia hortensis |
| topic | cadmium and copper JC-1 annexin V-FITC mitochondrial membrane depolarization phosphatidylserine translocation caspases |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/240 |
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