Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl
Metsulfuron-methyl, a widely used herbicide, could cause damage to the sensitive plants in crop-rotation systems at extremely low levels in the soil. The potential of plant growth-promoting bacteria (PGPB) for enhancing the resistance of plants against herbicide stress has been discovered recently....
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Journal of Xenobiotics |
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| Online Access: | https://www.mdpi.com/2039-4713/14/1/5 |
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| author | Margarita Bakaeva Sergey Chetverikov Sergey Starikov Aliya Kendjieva Gaisar Khudaygulov Darya Chetverikova |
| author_facet | Margarita Bakaeva Sergey Chetverikov Sergey Starikov Aliya Kendjieva Gaisar Khudaygulov Darya Chetverikova |
| author_sort | Margarita Bakaeva |
| collection | DOAJ |
| description | Metsulfuron-methyl, a widely used herbicide, could cause damage to the sensitive plants in crop-rotation systems at extremely low levels in the soil. The potential of plant growth-promoting bacteria (PGPB) for enhancing the resistance of plants against herbicide stress has been discovered recently. Therefore, it is poorly understood how physiological processes occur in plants, while PGPB reduce the phytotoxicity of herbicides for agricultural crops. In greenhouse studies, the effect of strains <i>Pseudomonas protegens</i> DA1.2 and <i>Pseudomonas chlororaphis</i> 4CH on oxidative damage, acetolactate synthase (ALS), enzymatic and non-enzymatic antioxidants in canola (<i>Brassica napus</i> L.), and wheat (<i>Triticum aestivum</i> L.) were investigated under two levels (0.05 and 0.25 mg∙kg<sup>−1</sup>) of metsulfuron-methyl using spectrophotometric assays. The inoculation of herbicide-exposed wheat with bacteria significantly increased the shoots fresh weight (24–28%), amount of glutathione GSH (60–73%), and flavonoids (5–14%), as well as activity of ascorbate peroxidase (129–140%), superoxide dismutase SOD (35–49%), and ALS (50–57%). Bacterial treatment stimulated the activity of SOD (37–94%), ALS (65–73%), glutathione reductase (19–20%), and the accumulation of GSH (61–261%), flavonoids (17–22%), and shoots weight (27–33%) in herbicide-exposed canola. Simultaneous inoculation prevented lipid peroxidation induced by metsulfuron-methyl in sensitive plants. Based on the findings, it is possible that the protective role of bacterial strains against metsulfuron-metil is linked to antioxidant system activation. |
| first_indexed | 2024-04-24T18:07:12Z |
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| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-04-24T18:07:12Z |
| publishDate | 2024-01-01 |
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| series | Journal of Xenobiotics |
| spelling | doaj.art-92ff0dcbce8649239f59fdbd2983fe412024-03-27T13:49:55ZengMDPI AGJournal of Xenobiotics2039-47052039-47132024-01-01141799510.3390/jox14010005Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-MethylMargarita Bakaeva0Sergey Chetverikov1Sergey Starikov2Aliya Kendjieva3Gaisar Khudaygulov4Darya Chetverikova5Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, RussiaMetsulfuron-methyl, a widely used herbicide, could cause damage to the sensitive plants in crop-rotation systems at extremely low levels in the soil. The potential of plant growth-promoting bacteria (PGPB) for enhancing the resistance of plants against herbicide stress has been discovered recently. Therefore, it is poorly understood how physiological processes occur in plants, while PGPB reduce the phytotoxicity of herbicides for agricultural crops. In greenhouse studies, the effect of strains <i>Pseudomonas protegens</i> DA1.2 and <i>Pseudomonas chlororaphis</i> 4CH on oxidative damage, acetolactate synthase (ALS), enzymatic and non-enzymatic antioxidants in canola (<i>Brassica napus</i> L.), and wheat (<i>Triticum aestivum</i> L.) were investigated under two levels (0.05 and 0.25 mg∙kg<sup>−1</sup>) of metsulfuron-methyl using spectrophotometric assays. The inoculation of herbicide-exposed wheat with bacteria significantly increased the shoots fresh weight (24–28%), amount of glutathione GSH (60–73%), and flavonoids (5–14%), as well as activity of ascorbate peroxidase (129–140%), superoxide dismutase SOD (35–49%), and ALS (50–57%). Bacterial treatment stimulated the activity of SOD (37–94%), ALS (65–73%), glutathione reductase (19–20%), and the accumulation of GSH (61–261%), flavonoids (17–22%), and shoots weight (27–33%) in herbicide-exposed canola. Simultaneous inoculation prevented lipid peroxidation induced by metsulfuron-methyl in sensitive plants. Based on the findings, it is possible that the protective role of bacterial strains against metsulfuron-metil is linked to antioxidant system activation.https://www.mdpi.com/2039-4713/14/1/5acetolactate synthaseantioxidantsantioxidant enzymesbacteriabiostimulantherbicide |
| spellingShingle | Margarita Bakaeva Sergey Chetverikov Sergey Starikov Aliya Kendjieva Gaisar Khudaygulov Darya Chetverikova Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl Journal of Xenobiotics acetolactate synthase antioxidants antioxidant enzymes bacteria biostimulant herbicide |
| title | Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl |
| title_full | Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl |
| title_fullStr | Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl |
| title_full_unstemmed | Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl |
| title_short | Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl |
| title_sort | effect of plant growth promoting bacteria on antioxidant status acetolactate synthase activity and growth of common wheat and canola exposed to metsulfuron methyl |
| topic | acetolactate synthase antioxidants antioxidant enzymes bacteria biostimulant herbicide |
| url | https://www.mdpi.com/2039-4713/14/1/5 |
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