Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS
A method based on a QuEChERS sample preparation approach coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established to determine six aflatoxins (B1, B2, G1, G2, M1, M2) residues in grains. Samples were extracted with acetonitrile –0.1% formic acid aque...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2024-03-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240219 |
| _version_ | 1827304410406977536 |
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| author | WU Qin-yan ZHANG Wen-wen LIANG Hong-fang WANG Jian-hua CHEN Lu |
| author_facet | WU Qin-yan ZHANG Wen-wen LIANG Hong-fang WANG Jian-hua CHEN Lu |
| author_sort | WU Qin-yan |
| collection | DOAJ |
| description | A method based on a QuEChERS sample preparation approach coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established to determine six aflatoxins (B1, B2, G1, G2, M1, M2) residues in grains. Samples were extracted with acetonitrile –0.1% formic acid aqueous solution (84 : 16, v/v), followed by purifying with PSA, and determined by UPLC-MS/MS in multiple reaction detection mode with 0.1% formic acid-acetonitrile as mobile phase. The correlation coefficients of the standard curves of the six aflatoxins were all greater than 0.99, showing good linearity. The recoveries were 80.83%~117.5%, and the relative standard deviations (RSD) were 1.53%~15.59%. The matrix effects of six aflatoxins were 0.81~1.12 in different varieties of grains. The accuracy and precision of this method meet the requirements of relevant standards, and have the advantages of simple pretreatment technology, good purification effect and high precision determination, which could be suitable for the quantitative analysis of six aflatoxins in wheat, rice, corn, mung bean and red bean samples. |
| first_indexed | 2024-04-24T17:35:10Z |
| format | Article |
| id | doaj.art-92ff419da3bc4cb4852d0c5ab6c8b68c |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-24T17:35:10Z |
| publishDate | 2024-03-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-92ff419da3bc4cb4852d0c5ab6c8b68c2024-03-28T06:00:47ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612024-03-0132214515010.16210/j.cnki.1007-7561.2024.02.018Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MSWU Qin-yan0ZHANG Wen-wen1 LIANG Hong-fang2WANG Jian-hua3CHEN Lu4Central Laboratory of Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province, Jurong, Jiangsu 212400, ChinaZhenjiang Agricultural Products Quality Inspection and Testing Center, Zhenjiang, Jiangsu 212009, ChinaCentral Laboratory of Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province, Jurong, Jiangsu 212400, ChinaCentral Laboratory of Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province, Jurong, Jiangsu 212400, ChinaCentral Laboratory of Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province, Jurong, Jiangsu 212400, ChinaA method based on a QuEChERS sample preparation approach coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established to determine six aflatoxins (B1, B2, G1, G2, M1, M2) residues in grains. Samples were extracted with acetonitrile –0.1% formic acid aqueous solution (84 : 16, v/v), followed by purifying with PSA, and determined by UPLC-MS/MS in multiple reaction detection mode with 0.1% formic acid-acetonitrile as mobile phase. The correlation coefficients of the standard curves of the six aflatoxins were all greater than 0.99, showing good linearity. The recoveries were 80.83%~117.5%, and the relative standard deviations (RSD) were 1.53%~15.59%. The matrix effects of six aflatoxins were 0.81~1.12 in different varieties of grains. The accuracy and precision of this method meet the requirements of relevant standards, and have the advantages of simple pretreatment technology, good purification effect and high precision determination, which could be suitable for the quantitative analysis of six aflatoxins in wheat, rice, corn, mung bean and red bean samples.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240219quechersuplc-ms/msgrainsaflatoxins |
| spellingShingle | WU Qin-yan ZHANG Wen-wen LIANG Hong-fang WANG Jian-hua CHEN Lu Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS Liang you shipin ke-ji quechers uplc-ms/ms grains aflatoxins |
| title | Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS |
| title_full | Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS |
| title_fullStr | Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS |
| title_full_unstemmed | Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS |
| title_short | Determination of Aflatoxins in Grains by QuEChERS-UPLC-MS/MS |
| title_sort | determination of aflatoxins in grains by quechers uplc ms ms |
| topic | quechers uplc-ms/ms grains aflatoxins |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240219 |
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