Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection
<i>Salmonella enterica</i> serovar Typhimurium (ST) is a food-borne pathogen that can infect animals and humans. It is currently the most common bacterial pathogen that negatively affects the poultry industry. Although different chicken breeds have been observed to exhibit diverse resist...
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| author | Zixuan Wang Hailong Wang Astrid Lissette Barreto Sánchez Mamadou Thiam Jin Zhang Qinghe Li Maiqing Zheng Jie Wen Hegang Li Guiping Zhao Qiao Wang |
| author_facet | Zixuan Wang Hailong Wang Astrid Lissette Barreto Sánchez Mamadou Thiam Jin Zhang Qinghe Li Maiqing Zheng Jie Wen Hegang Li Guiping Zhao Qiao Wang |
| author_sort | Zixuan Wang |
| collection | DOAJ |
| description | <i>Salmonella enterica</i> serovar Typhimurium (ST) is a food-borne pathogen that can infect animals and humans. It is currently the most common bacterial pathogen that negatively affects the poultry industry. Although different chicken breeds have been observed to exhibit diverse resistance to ST infection, the underlying genetic mechanisms remain unclear and the genes involved in this differential disease resistance need to be identified. To overcome this knowledge gap, we used a liver transcriptome analysis to screen differentially expressed genes (DEGs) in two different chicken breeds (local Beijing You (BY) and commercial Guang Ming No. 2 broiler line B (GM)) before and after ST infection. We also performed weighted gene co-expression network analysis (WGCNA) to detect hub genes, and employed selection signal analysis of candidate genes. Three promising genes (<i>EGR1</i>, <i>JUN</i> and <i>FOS</i>) were eventually identified, and were significantly and differentially expressed in the same breed under different conditions, and in the two breeds after ST infection. Hub genes, such as <i>PPFIA4</i> and <i>ZNF395</i>, were identified using WGCNA, and were associated with the ratio of heterophils to lymphocytes (H/L), an indicator of disease resistance. the present study identified several genes and pathways associated with resistance to ST infection, and found that BY had greater resistance to ST infection than GM. The results obtained provide valuable resources for investigating the mechanisms of resistance to ST infection in different chicken breeds. |
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| id | doaj.art-93034302cb994bcfacf1a8a4ef271589 |
| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T16:04:24Z |
| publishDate | 2022-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-93034302cb994bcfacf1a8a4ef2715892023-11-24T16:49:08ZengMDPI AGMicroorganisms2076-26072022-12-011012244010.3390/microorganisms10122440Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium InfectionZixuan Wang0Hailong Wang1Astrid Lissette Barreto Sánchez2Mamadou Thiam3Jin Zhang4Qinghe Li5Maiqing Zheng6Jie Wen7Hegang Li8Guiping Zhao9Qiao Wang10Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China<i>Salmonella enterica</i> serovar Typhimurium (ST) is a food-borne pathogen that can infect animals and humans. It is currently the most common bacterial pathogen that negatively affects the poultry industry. Although different chicken breeds have been observed to exhibit diverse resistance to ST infection, the underlying genetic mechanisms remain unclear and the genes involved in this differential disease resistance need to be identified. To overcome this knowledge gap, we used a liver transcriptome analysis to screen differentially expressed genes (DEGs) in two different chicken breeds (local Beijing You (BY) and commercial Guang Ming No. 2 broiler line B (GM)) before and after ST infection. We also performed weighted gene co-expression network analysis (WGCNA) to detect hub genes, and employed selection signal analysis of candidate genes. Three promising genes (<i>EGR1</i>, <i>JUN</i> and <i>FOS</i>) were eventually identified, and were significantly and differentially expressed in the same breed under different conditions, and in the two breeds after ST infection. Hub genes, such as <i>PPFIA4</i> and <i>ZNF395</i>, were identified using WGCNA, and were associated with the ratio of heterophils to lymphocytes (H/L), an indicator of disease resistance. the present study identified several genes and pathways associated with resistance to ST infection, and found that BY had greater resistance to ST infection than GM. The results obtained provide valuable resources for investigating the mechanisms of resistance to ST infection in different chicken breeds.https://www.mdpi.com/2076-2607/10/12/2440<i>Salmonella enterica</i> serovar Typhimuriumdisease resistancetranscriptome analysisWGCNAselection signature |
| spellingShingle | Zixuan Wang Hailong Wang Astrid Lissette Barreto Sánchez Mamadou Thiam Jin Zhang Qinghe Li Maiqing Zheng Jie Wen Hegang Li Guiping Zhao Qiao Wang Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection Microorganisms <i>Salmonella enterica</i> serovar Typhimurium disease resistance transcriptome analysis WGCNA selection signature |
| title | Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection |
| title_full | Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection |
| title_fullStr | Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection |
| title_full_unstemmed | Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection |
| title_short | Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under <i>Salmonella enterica</i> Serovar Typhimurium Infection |
| title_sort | comparative analysis of the liver transcriptome of beijing you chickens and guang ming broilers under i salmonella enterica i serovar typhimurium infection |
| topic | <i>Salmonella enterica</i> serovar Typhimurium disease resistance transcriptome analysis WGCNA selection signature |
| url | https://www.mdpi.com/2076-2607/10/12/2440 |
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