Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms
Acute gouty arthritis is an acute inflammatory reaction caused by the deposition of monosodium urate (MSU) crystals in joints and surrounding soft tissues. Controlling inflammation is the key to preventing acute gouty arthritis. Anti-inflammatory activities and the possible molecular mechanisms of p...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/2/254 |
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| author | Yibin Li Wei Deng Li Wu Shouhui Chen Zhipeng Zheng Hongbo Song |
| author_facet | Yibin Li Wei Deng Li Wu Shouhui Chen Zhipeng Zheng Hongbo Song |
| author_sort | Yibin Li |
| collection | DOAJ |
| description | Acute gouty arthritis is an acute inflammatory reaction caused by the deposition of monosodium urate (MSU) crystals in joints and surrounding soft tissues. Controlling inflammation is the key to preventing acute gouty arthritis. Anti-inflammatory activities and the possible molecular mechanisms of plum (<i>Prunus salicina Lindl</i> cv. “furong”) polyphenols (PSLP) on RAW264.7 macrophage cells induced by monosodium urate were investigated. PPSF significantly inhibited the activity of inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-18 (IL-18). In addition, PPSF exhibited excellent activation of superoxide dismutase (SOD) activity and reduction of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels in RAW264.7 macrophages. The results of global screening of all transcripts by RNA-seq revealed 8585 differentially expressed genes between the PSLP-treated group and the MUS group. From GO analysis, PSLP could affect the occurrence and development of RAW264.7 macrophage inflammation through biological processes, such as organic substance metabolism, intracellular organelles, and binding function. The regulation mechanism of PSLP on MSU-induced RAW264.7 macrophage inflammation may be achieved through the HIF-1 signaling pathway, renal cell carcinoma, the ErbB signaling pathway, and the FoxO signaling pathway. Therefore, PSLP has great prospects in the prevention of gout and similar inflammatory diseases. |
| first_indexed | 2024-03-09T12:45:03Z |
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| id | doaj.art-9303dacb47974a2badc81cdff4d7ce92 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:45:03Z |
| publishDate | 2023-01-01 |
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| series | Foods |
| spelling | doaj.art-9303dacb47974a2badc81cdff4d7ce922023-11-30T22:13:43ZengMDPI AGFoods2304-81582023-01-0112225410.3390/foods12020254Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential MechanismsYibin Li0Wei Deng1Li Wu2Shouhui Chen3Zhipeng Zheng4Hongbo Song5College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaResearch Institute of Agri-Engineering and Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, ChinaResearch Institute of Agri-Engineering and Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaAcute gouty arthritis is an acute inflammatory reaction caused by the deposition of monosodium urate (MSU) crystals in joints and surrounding soft tissues. Controlling inflammation is the key to preventing acute gouty arthritis. Anti-inflammatory activities and the possible molecular mechanisms of plum (<i>Prunus salicina Lindl</i> cv. “furong”) polyphenols (PSLP) on RAW264.7 macrophage cells induced by monosodium urate were investigated. PPSF significantly inhibited the activity of inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-18 (IL-18). In addition, PPSF exhibited excellent activation of superoxide dismutase (SOD) activity and reduction of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels in RAW264.7 macrophages. The results of global screening of all transcripts by RNA-seq revealed 8585 differentially expressed genes between the PSLP-treated group and the MUS group. From GO analysis, PSLP could affect the occurrence and development of RAW264.7 macrophage inflammation through biological processes, such as organic substance metabolism, intracellular organelles, and binding function. The regulation mechanism of PSLP on MSU-induced RAW264.7 macrophage inflammation may be achieved through the HIF-1 signaling pathway, renal cell carcinoma, the ErbB signaling pathway, and the FoxO signaling pathway. Therefore, PSLP has great prospects in the prevention of gout and similar inflammatory diseases.https://www.mdpi.com/2304-8158/12/2/254<i>Prunus salicina Lindl</i>polyphenolsRNA-seqpathways |
| spellingShingle | Yibin Li Wei Deng Li Wu Shouhui Chen Zhipeng Zheng Hongbo Song Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms Foods <i>Prunus salicina Lindl</i> polyphenols RNA-seq pathways |
| title | Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms |
| title_full | Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms |
| title_fullStr | Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms |
| title_full_unstemmed | Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms |
| title_short | Anti-Inflammatory Effects of Polyphenols from Plum (<i>Prunus salicina Lindl</i>) on RAW264.7 Macrophages Induced by Monosodium Urate and Potential Mechanisms |
| title_sort | anti inflammatory effects of polyphenols from plum i prunus salicina lindl i on raw264 7 macrophages induced by monosodium urate and potential mechanisms |
| topic | <i>Prunus salicina Lindl</i> polyphenols RNA-seq pathways |
| url | https://www.mdpi.com/2304-8158/12/2/254 |
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