Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise.
Inflammation is a complex, biologic event that aims to protect and repair tissue. Previous studies suggest that inflammation is critical to induce a healing response following acute injury; however, whether similar inflammatory responses occur as a result of beneficial, non-injurious loading is unkn...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4598142?pdf=render |
| _version_ | 1818848212960673792 |
|---|---|
| author | Sarah Ilkhanipour Rooney John W Tobias Pankti R Bhatt Andrew F Kuntz Louis J Soslowsky |
| author_facet | Sarah Ilkhanipour Rooney John W Tobias Pankti R Bhatt Andrew F Kuntz Louis J Soslowsky |
| author_sort | Sarah Ilkhanipour Rooney |
| collection | DOAJ |
| description | Inflammation is a complex, biologic event that aims to protect and repair tissue. Previous studies suggest that inflammation is critical to induce a healing response following acute injury; however, whether similar inflammatory responses occur as a result of beneficial, non-injurious loading is unknown. The objective of this study was to screen for alterations in a subset of inflammatory and extracellular matrix genes to identify the responses of rat supraspinatus tendon and muscle to a known, non-injurious loading condition. We sought to define how a subset of genes representative of specific inflammation and matrix turnover pathways is altered in supraspinatus tendon and muscle 1) acutely following a single loading bout and 2) chronically following repeated loading bouts. In this study, Sprague-Dawley rats in the acute group ran a single bout of non-injurious exercise on a flat treadmill (10 m/min, 1 hour) and were sacrificed 12 or 24 hours after. Rats in the chronic group ran 5 days/wk for 1 or 8 weeks. A control group maintained normal cage activity. Supraspinatus muscle and tendon were harvested for RNA extractions, and a custom Panomics QuantiGene 2.0 multiplex assay was used to detect 48 target and 3 housekeeping genes. Muscle/tendon and acute/chronic groups had distinct gene expression. Components of the arachidonic acid cascade and matrix metalloproteinases and their inhibitors were altered with acute and chronic exercise. Collagen expression increased. Using a previously validated model of non-injurious exercise, we have shown that supraspinatus tendon and muscle respond to acute and chronic exercise by regulating inflammatory- and matrix turnover-related genes, suggesting that these pathways are involved in the beneficial adaptations to exercise. |
| first_indexed | 2024-12-19T06:13:46Z |
| format | Article |
| id | doaj.art-93040450fb3d40e2820bed7f920f614f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T06:13:46Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-93040450fb3d40e2820bed7f920f614f2022-12-21T20:32:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e013988010.1371/journal.pone.0139880Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise.Sarah Ilkhanipour RooneyJohn W TobiasPankti R BhattAndrew F KuntzLouis J SoslowskyInflammation is a complex, biologic event that aims to protect and repair tissue. Previous studies suggest that inflammation is critical to induce a healing response following acute injury; however, whether similar inflammatory responses occur as a result of beneficial, non-injurious loading is unknown. The objective of this study was to screen for alterations in a subset of inflammatory and extracellular matrix genes to identify the responses of rat supraspinatus tendon and muscle to a known, non-injurious loading condition. We sought to define how a subset of genes representative of specific inflammation and matrix turnover pathways is altered in supraspinatus tendon and muscle 1) acutely following a single loading bout and 2) chronically following repeated loading bouts. In this study, Sprague-Dawley rats in the acute group ran a single bout of non-injurious exercise on a flat treadmill (10 m/min, 1 hour) and were sacrificed 12 or 24 hours after. Rats in the chronic group ran 5 days/wk for 1 or 8 weeks. A control group maintained normal cage activity. Supraspinatus muscle and tendon were harvested for RNA extractions, and a custom Panomics QuantiGene 2.0 multiplex assay was used to detect 48 target and 3 housekeeping genes. Muscle/tendon and acute/chronic groups had distinct gene expression. Components of the arachidonic acid cascade and matrix metalloproteinases and their inhibitors were altered with acute and chronic exercise. Collagen expression increased. Using a previously validated model of non-injurious exercise, we have shown that supraspinatus tendon and muscle respond to acute and chronic exercise by regulating inflammatory- and matrix turnover-related genes, suggesting that these pathways are involved in the beneficial adaptations to exercise.http://europepmc.org/articles/PMC4598142?pdf=render |
| spellingShingle | Sarah Ilkhanipour Rooney John W Tobias Pankti R Bhatt Andrew F Kuntz Louis J Soslowsky Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. PLoS ONE |
| title | Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. |
| title_full | Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. |
| title_fullStr | Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. |
| title_full_unstemmed | Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. |
| title_short | Genetic Response of Rat Supraspinatus Tendon and Muscle to Exercise. |
| title_sort | genetic response of rat supraspinatus tendon and muscle to exercise |
| url | http://europepmc.org/articles/PMC4598142?pdf=render |
| work_keys_str_mv | AT sarahilkhanipourrooney geneticresponseofratsupraspinatustendonandmuscletoexercise AT johnwtobias geneticresponseofratsupraspinatustendonandmuscletoexercise AT panktirbhatt geneticresponseofratsupraspinatustendonandmuscletoexercise AT andrewfkuntz geneticresponseofratsupraspinatustendonandmuscletoexercise AT louisjsoslowsky geneticresponseofratsupraspinatustendonandmuscletoexercise |