Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction
Abstract Surface engineering of metal–organic framework nanoparticles (MOF NPs), and enabling their post‐synthetic modulation that facilitates the formation of bio‐interfaces has tremendous potential for diverse applications including therapeutics, imaging, biosensing, and drug‐delivery systems. Des...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-09-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202300048 |
| _version_ | 1797685027013656576 |
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| author | Ilona Wagner Simon Spiegel Julian Brückel Matthias Schwotzer Alexander Welle Martina H. Stenzel Stefan Bräse Salma Begum Manuel Tsotsalas |
| author_facet | Ilona Wagner Simon Spiegel Julian Brückel Matthias Schwotzer Alexander Welle Martina H. Stenzel Stefan Bräse Salma Begum Manuel Tsotsalas |
| author_sort | Ilona Wagner |
| collection | DOAJ |
| description | Abstract Surface engineering of metal–organic framework nanoparticles (MOF NPs), and enabling their post‐synthetic modulation that facilitates the formation of bio‐interfaces has tremendous potential for diverse applications including therapeutics, imaging, biosensing, and drug‐delivery systems. Despite the progress in MOF NPs synthesis, colloidal stability and homogeneous dispersity—a desirable property for biotechnological applications, stands as a critical obstacle and remains a challenging task. In this report, dynamic surfaces modification of MOF NPs with polyethylene glycol (PEG) polymer is described using grafting‐from PEGylation by employing nitroxide‐mediated polymerization (NMP) and inserting arginylglycylaspartic acid (RGD) peptides on the surface via a nitroxide exchange reaction (NER). The dynamic modification strategy enables tailoring PEG‐grafted MOF NPs of the type UiO‐66‐NH2 with improved colloidal stability, and high dispersity, while the morphology and lattice crystallinity are strictly preserved. The interaction of PEG‐grafted MOF NPs with human serum albumin (HSA) protein under physiological conditions is studied. The PEG‐grafted colloidal MOF NPs adsorb less HSA protein than the uncoated ones. Therefore, the described approach increases the scope of bio‐relevant applications of colloidal MOF NPs by reducing nonspecific interactions using NMP based PEGylation, while preserving the possibility to introduce targeting moieties via NER for specific interactions. |
| first_indexed | 2024-03-12T00:39:18Z |
| format | Article |
| id | doaj.art-93045e41d54b414d8040d20cd0375161 |
| institution | Directory Open Access Journal |
| issn | 1438-7492 1439-2054 |
| language | English |
| last_indexed | 2024-03-12T00:39:18Z |
| publishDate | 2023-09-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj.art-93045e41d54b414d8040d20cd03751612023-09-15T09:14:11ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542023-09-013089n/an/a10.1002/mame.202300048Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange ReactionIlona Wagner0Simon Spiegel1Julian Brückel2Matthias Schwotzer3Alexander Welle4Martina H. Stenzel5Stefan Bräse6Salma Begum7Manuel Tsotsalas8Institute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyInstitute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyInstitute for Organic Chemistry (IOC), Karlsruhe Institute of Technology (KIT) Fritz‐Haber‐Weg 6 76131 Karlsruhe GermanyInstitute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyInstitute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyCenter for Advanced Macromolecular Design, School of Chemistry University of New South Wales Sydney NSW 2052 AustraliaInstitute for Organic Chemistry (IOC), Karlsruhe Institute of Technology (KIT) Fritz‐Haber‐Weg 6 76131 Karlsruhe GermanyInstitute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyInstitute of Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Hermann‐von Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen GermanyAbstract Surface engineering of metal–organic framework nanoparticles (MOF NPs), and enabling their post‐synthetic modulation that facilitates the formation of bio‐interfaces has tremendous potential for diverse applications including therapeutics, imaging, biosensing, and drug‐delivery systems. Despite the progress in MOF NPs synthesis, colloidal stability and homogeneous dispersity—a desirable property for biotechnological applications, stands as a critical obstacle and remains a challenging task. In this report, dynamic surfaces modification of MOF NPs with polyethylene glycol (PEG) polymer is described using grafting‐from PEGylation by employing nitroxide‐mediated polymerization (NMP) and inserting arginylglycylaspartic acid (RGD) peptides on the surface via a nitroxide exchange reaction (NER). The dynamic modification strategy enables tailoring PEG‐grafted MOF NPs of the type UiO‐66‐NH2 with improved colloidal stability, and high dispersity, while the morphology and lattice crystallinity are strictly preserved. The interaction of PEG‐grafted MOF NPs with human serum albumin (HSA) protein under physiological conditions is studied. The PEG‐grafted colloidal MOF NPs adsorb less HSA protein than the uncoated ones. Therefore, the described approach increases the scope of bio‐relevant applications of colloidal MOF NPs by reducing nonspecific interactions using NMP based PEGylation, while preserving the possibility to introduce targeting moieties via NER for specific interactions.https://doi.org/10.1002/mame.202300048MOF nanoparticlesnitroxide exchange reactionnitroxide‐mediated polymerizationpost‐synthetic modificationsurface functionalization |
| spellingShingle | Ilona Wagner Simon Spiegel Julian Brückel Matthias Schwotzer Alexander Welle Martina H. Stenzel Stefan Bräse Salma Begum Manuel Tsotsalas Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction Macromolecular Materials and Engineering MOF nanoparticles nitroxide exchange reaction nitroxide‐mediated polymerization post‐synthetic modification surface functionalization |
| title | Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction |
| title_full | Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction |
| title_fullStr | Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction |
| title_full_unstemmed | Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction |
| title_short | Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction |
| title_sort | biofunctionalization of metal organic framework nanoparticles via combined nitroxide mediated polymerization and nitroxide exchange reaction |
| topic | MOF nanoparticles nitroxide exchange reaction nitroxide‐mediated polymerization post‐synthetic modification surface functionalization |
| url | https://doi.org/10.1002/mame.202300048 |
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