Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells
We evaluated the expression of the costimulatory moleculesCD80 and CD83 and major histocompatibility (MHC) class IIinduced by 2,4-dinitrofluorobenzene (DNFB) in the RAW264.7 macrophage cell line. In contrast to the previously reportedeffect of DNFB on dendritic cells, CD86 expression didnot change....
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| Format: | Article |
| Language: | English |
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Korean Society for Biochemistry and Molecular Biology
2012-09-01
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| Series: | BMB Reports |
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| Online Access: | http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTU0Ml8lMjg1MzgtNTQzJTI5Qk1CXzEyLTA0OC5wZGY= |
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| author | Dongbum Kim1, Min Chul Park1, Byoung Kwon Park1, Sanghoon Kwon2, Joon-Ho Choi3, Hyun-Jong Kim4, Soo-Young Choi5, Jinseu Park5, Younghee Lee6 & Hyung-Joo Kwon1,2,* |
| author_facet | Dongbum Kim1, Min Chul Park1, Byoung Kwon Park1, Sanghoon Kwon2, Joon-Ho Choi3, Hyun-Jong Kim4, Soo-Young Choi5, Jinseu Park5, Younghee Lee6 & Hyung-Joo Kwon1,2,* |
| author_sort | Dongbum Kim1, Min Chul Park1, Byoung Kwon Park1, Sanghoon Kwon2, Joon-Ho Choi3, Hyun-Jong Kim4, Soo-Young Choi5, Jinseu Park5, Younghee Lee6 & Hyung-Joo Kwon1,2,* |
| collection | DOAJ |
| description | We evaluated the expression of the costimulatory moleculesCD80 and CD83 and major histocompatibility (MHC) class IIinduced by 2,4-dinitrofluorobenzene (DNFB) in the RAW264.7 macrophage cell line. In contrast to the previously reportedeffect of DNFB on dendritic cells, CD86 expression didnot change. Furthermore, we observed that the CD83 expressionlevel transiently increased and then decreased.Induction of CD80 and MHC class II molecule expression anda decrease in CD83 expression by DNFB in vitro were alsoconfirmed in splenocytes of BALB/c and NC/Nga mice.However, DNFB did not influence CD83 expression in peritonealCD11b+ cells from BALB/c or NC/Nga mice. Detailedin vivo experiments and further studies on the possible contributionof CD11b+ cells to induce atopic dermatitis (AD)would be helpful to attain a better understanding of ADpathogenesis. |
| first_indexed | 2024-12-10T14:10:55Z |
| format | Article |
| id | doaj.art-9305724d0b044a968a74ce47d80d3e0b |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-12-10T14:10:55Z |
| publishDate | 2012-09-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-9305724d0b044a968a74ce47d80d3e0b2022-12-22T01:45:30ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2012-09-01459538543Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cellsDongbum Kim1, Min Chul Park1, Byoung Kwon Park1, Sanghoon Kwon2, Joon-Ho Choi3, Hyun-Jong Kim4, Soo-Young Choi5, Jinseu Park5, Younghee Lee6 & Hyung-Joo Kwon1,2,*We evaluated the expression of the costimulatory moleculesCD80 and CD83 and major histocompatibility (MHC) class IIinduced by 2,4-dinitrofluorobenzene (DNFB) in the RAW264.7 macrophage cell line. In contrast to the previously reportedeffect of DNFB on dendritic cells, CD86 expression didnot change. Furthermore, we observed that the CD83 expressionlevel transiently increased and then decreased.Induction of CD80 and MHC class II molecule expression anda decrease in CD83 expression by DNFB in vitro were alsoconfirmed in splenocytes of BALB/c and NC/Nga mice.However, DNFB did not influence CD83 expression in peritonealCD11b+ cells from BALB/c or NC/Nga mice. Detailedin vivo experiments and further studies on the possible contributionof CD11b+ cells to induce atopic dermatitis (AD)would be helpful to attain a better understanding of ADpathogenesis.http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTU0Ml8lMjg1MzgtNTQzJTI5Qk1CXzEyLTA0OC5wZGY=Atopic dermatitisCostimulatory moleculesDNFBMacrophagesNC/Nga mice |
| spellingShingle | Dongbum Kim1, Min Chul Park1, Byoung Kwon Park1, Sanghoon Kwon2, Joon-Ho Choi3, Hyun-Jong Kim4, Soo-Young Choi5, Jinseu Park5, Younghee Lee6 & Hyung-Joo Kwon1,2,* Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells BMB Reports Atopic dermatitis Costimulatory molecules DNFB Macrophages NC/Nga mice |
| title | Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells |
| title_full | Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells |
| title_fullStr | Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells |
| title_full_unstemmed | Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells |
| title_short | Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells |
| title_sort | differential expression of cell surface markers in response to 2 4 dinitrofluorobenzene in raw 264 7 and primary immune cells |
| topic | Atopic dermatitis Costimulatory molecules DNFB Macrophages NC/Nga mice |
| url | http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTU0Ml8lMjg1MzgtNTQzJTI5Qk1CXzEyLTA0OC5wZGY= |
| work_keys_str_mv | AT dongbumkim1minchulpark1byoungkwonpark1sanghoonkwon2joonhochoi3hyunjongkim4sooyoungchoi5jinseupark5youngheelee6hyungjookwon12 differentialexpressionofcellsurfacemarkersinresponseto24dinitrofluorobenzeneinraw2647andprimaryimmunecells |