Gelsolin Inhibits the Inflammatory Process Induced by LPS
Background/Aims: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. Methods: IL-6 and TNF-α in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2017-01-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/456043 |
| _version_ | 1818475453326819328 |
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| author | Ying Cheng Xuguang Hu Cong Liu Ming Chen Jianing Wang Mingyu Wang Fu Gao Jiaqi Han Chaoxiong Zhang Ding Sun Rui Min |
| author_facet | Ying Cheng Xuguang Hu Cong Liu Ming Chen Jianing Wang Mingyu Wang Fu Gao Jiaqi Han Chaoxiong Zhang Ding Sun Rui Min |
| author_sort | Ying Cheng |
| collection | DOAJ |
| description | Background/Aims: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. Methods: IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine. Results: LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPS-induced death, and si-GSN increased death in the LPS-pretreated mice. Conclusion: GSN exhibited a protective role in endotoxemia. |
| first_indexed | 2024-12-10T09:12:46Z |
| format | Article |
| id | doaj.art-93061b0170fc44d0b1e7dc6adfd891c8 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T09:12:46Z |
| publishDate | 2017-01-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-93061b0170fc44d0b1e7dc6adfd891c82022-12-22T01:54:58ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-01-0141120521210.1159/000456043456043Gelsolin Inhibits the Inflammatory Process Induced by LPSYing ChengXuguang HuCong LiuMing ChenJianing WangMingyu WangFu GaoJiaqi HanChaoxiong ZhangDing SunRui MinBackground/Aims: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. Methods: IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine. Results: LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPS-induced death, and si-GSN increased death in the LPS-pretreated mice. Conclusion: GSN exhibited a protective role in endotoxemia.http://www.karger.com/Article/FullText/456043TNF-αLPSIL-6Gelsolin |
| spellingShingle | Ying Cheng Xuguang Hu Cong Liu Ming Chen Jianing Wang Mingyu Wang Fu Gao Jiaqi Han Chaoxiong Zhang Ding Sun Rui Min Gelsolin Inhibits the Inflammatory Process Induced by LPS Cellular Physiology and Biochemistry TNF-α LPS IL-6 Gelsolin |
| title | Gelsolin Inhibits the Inflammatory Process Induced by LPS |
| title_full | Gelsolin Inhibits the Inflammatory Process Induced by LPS |
| title_fullStr | Gelsolin Inhibits the Inflammatory Process Induced by LPS |
| title_full_unstemmed | Gelsolin Inhibits the Inflammatory Process Induced by LPS |
| title_short | Gelsolin Inhibits the Inflammatory Process Induced by LPS |
| title_sort | gelsolin inhibits the inflammatory process induced by lps |
| topic | TNF-α LPS IL-6 Gelsolin |
| url | http://www.karger.com/Article/FullText/456043 |
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