Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis
The genus Vibrio includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence An...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2023-03-01
|
| Series: | Italian Journal of Food Safety |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/ijfs/article/view/11045 |
| _version_ | 1811158004350844928 |
|---|---|
| author | Mohammad Shamsur Rahman Sarah Currò Lisa Carraro Barbara Cardazzo Stefania Balzan Enrico Novelli Federico Fontana Greta Caburlotto Amedeo Manfrin Luca Fasolato |
| author_facet | Mohammad Shamsur Rahman Sarah Currò Lisa Carraro Barbara Cardazzo Stefania Balzan Enrico Novelli Federico Fontana Greta Caburlotto Amedeo Manfrin Luca Fasolato |
| author_sort | Mohammad Shamsur Rahman |
| collection | DOAJ |
| description |
The genus Vibrio includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (gyrB, pyrH, recA and atpA) was applied to identify 92 Vibrio strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (e.g. V. parahaemolyticus). Use of the PubMLST Vibrio database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (V. alginolyticus) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of V. cholerae, V. vulnificus and V. parahaemolyticus, MLSA could be a valid support for species considered a minor risk, such as V. alginolyticus, V. mimicus and V. fluvialis. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of Open Science to protect the consumer.
|
| first_indexed | 2024-04-10T05:16:06Z |
| format | Article |
| id | doaj.art-930681a033084d27a938ffae4d1e0d21 |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-04-10T05:16:06Z |
| publishDate | 2023-03-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-930681a033084d27a938ffae4d1e0d212023-03-08T22:43:29ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322023-03-0112110.4081/ijfs.2023.11045Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysisMohammad Shamsur Rahman0Sarah Currò1Lisa Carraro2Barbara Cardazzo3Stefania Balzan4Enrico Novelli5Federico Fontana6Greta Caburlotto7Amedeo Manfrin8Luca Fasolato9Aquatic Animal Health Group, Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, DhakaDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroDepartment of Comparative Biomedicine and Food Science, University of Padua, LegnaroNational Reference Laboratory for Fish, Crustacean and Mollusc Pathologies, Istituto Zooprofilattico Sperimentale delle Venezie, PadovaNational Reference Laboratory for Fish, Crustacean and Mollusc Pathologies, Istituto Zooprofilattico Sperimentale delle Venezie, PadovaDepartment of Comparative Biomedicine and Food Science, University of Padua, Legnaro The genus Vibrio includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (gyrB, pyrH, recA and atpA) was applied to identify 92 Vibrio strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (e.g. V. parahaemolyticus). Use of the PubMLST Vibrio database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (V. alginolyticus) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of V. cholerae, V. vulnificus and V. parahaemolyticus, MLSA could be a valid support for species considered a minor risk, such as V. alginolyticus, V. mimicus and V. fluvialis. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of Open Science to protect the consumer. https://www.pagepressjournals.org/index.php/ijfs/article/view/11045Vibrio spp.crustaceanMLSAemerging risks |
| spellingShingle | Mohammad Shamsur Rahman Sarah Currò Lisa Carraro Barbara Cardazzo Stefania Balzan Enrico Novelli Federico Fontana Greta Caburlotto Amedeo Manfrin Luca Fasolato Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis Italian Journal of Food Safety Vibrio spp. crustacean MLSA emerging risks |
| title | Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis |
| title_full | Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis |
| title_fullStr | Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis |
| title_full_unstemmed | Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis |
| title_short | Retrospective analysis of <em>Vibrio</em> spp. isolated from marketed crustaceans using multilocus sequence analysis |
| title_sort | retrospective analysis of em vibrio em spp isolated from marketed crustaceans using multilocus sequence analysis |
| topic | Vibrio spp. crustacean MLSA emerging risks |
| url | https://www.pagepressjournals.org/index.php/ijfs/article/view/11045 |
| work_keys_str_mv | AT mohammadshamsurrahman retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT sarahcurro retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT lisacarraro retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT barbaracardazzo retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT stefaniabalzan retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT enriconovelli retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT federicofontana retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT gretacaburlotto retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT amedeomanfrin retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis AT lucafasolato retrospectiveanalysisofemvibrioemsppisolatedfrommarketedcrustaceansusingmultilocussequenceanalysis |