Dissecting mitosis by RNAi in Drosophila tissue culture cells
<p>Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference (RNAi) in cultured cells of <it>Drosophila melanogaster</it>. This procedure is particularly useful for the analysis of genes for which ge...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/57/m57.htm |
| _version_ | 1818484699120533504 |
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| author | Maiato Helder Sunkel Claudio E. Earnshaw William C. |
| author_facet | Maiato Helder Sunkel Claudio E. Earnshaw William C. |
| author_sort | Maiato Helder |
| collection | DOAJ |
| description | <p>Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference (RNAi) in cultured cells of <it>Drosophila melanogaster</it>. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST/Orbit as an example for the usefulness of the technique. |
| first_indexed | 2024-12-10T15:58:19Z |
| format | Article |
| id | doaj.art-9306a48c27d44c2bbe195c6518b6b477 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-10T15:58:19Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-9306a48c27d44c2bbe195c6518b6b4772022-12-22T01:42:34ZengBMCBiological Procedures Online1480-92222003-01-015115316110.1251/bpo57Dissecting mitosis by RNAi in Drosophila tissue culture cellsMaiato HelderSunkel Claudio E.Earnshaw William C.<p>Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference (RNAi) in cultured cells of <it>Drosophila melanogaster</it>. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST/Orbit as an example for the usefulness of the technique.http://www.biologicalprocedures.com/bpo/arts/1/57/m57.htmMitosisMicrotubulesKinetochoresDrosophila |
| spellingShingle | Maiato Helder Sunkel Claudio E. Earnshaw William C. Dissecting mitosis by RNAi in Drosophila tissue culture cells Biological Procedures Online Mitosis Microtubules Kinetochores Drosophila |
| title | Dissecting mitosis by RNAi in Drosophila tissue culture cells |
| title_full | Dissecting mitosis by RNAi in Drosophila tissue culture cells |
| title_fullStr | Dissecting mitosis by RNAi in Drosophila tissue culture cells |
| title_full_unstemmed | Dissecting mitosis by RNAi in Drosophila tissue culture cells |
| title_short | Dissecting mitosis by RNAi in Drosophila tissue culture cells |
| title_sort | dissecting mitosis by rnai in drosophila tissue culture cells |
| topic | Mitosis Microtubules Kinetochores Drosophila |
| url | http://www.biologicalprocedures.com/bpo/arts/1/57/m57.htm |
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