Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics
The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2014-12-01
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| Series: | Media Peternakan |
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| Online Access: | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/7969 |
| _version_ | 1828337850048839680 |
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| author | S. N. Jannah A. Dinoto K. G. Wiryawan I. Rusmana |
| author_facet | S. N. Jannah A. Dinoto K. G. Wiryawan I. Rusmana |
| author_sort | S. N. Jannah |
| collection | DOAJ |
| description | The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken. |
| first_indexed | 2024-04-13T22:20:11Z |
| format | Article |
| id | doaj.art-9307ccdd254b46f585bfab8da1ce5bcc |
| institution | Directory Open Access Journal |
| issn | 0126-0472 2087-4634 |
| language | English |
| last_indexed | 2024-04-13T22:20:11Z |
| publishDate | 2014-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Media Peternakan |
| spelling | doaj.art-9307ccdd254b46f585bfab8da1ce5bcc2022-12-22T02:27:18ZengBogor Agricultural UniversityMedia Peternakan0126-04722087-46342014-12-0137318218910.5398/medpet.2014.37.3.182Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as ProbioticsS. N. Jannah 0A. Dinoto 1K. G. Wiryawan 2I. Rusmana 3Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, IndonesiaResearch Center for Biology, Indonesian Institute of Sciences (LIPI) Department of Nutrition and Feed Tecnology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, IndonesiaThe aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/7969Cemani chickengastrointestinal tractlactic acid bacteriaprobiotic |
| spellingShingle | S. N. Jannah A. Dinoto K. G. Wiryawan I. Rusmana Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Media Peternakan Cemani chicken gastrointestinal tract lactic acid bacteria probiotic |
| title | Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics |
| title_full | Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics |
| title_fullStr | Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics |
| title_full_unstemmed | Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics |
| title_short | Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics |
| title_sort | characteristics of lactic acid bacteria isolated from gastrointestinal tract of cemani chicken and their potential use as probiotics |
| topic | Cemani chicken gastrointestinal tract lactic acid bacteria probiotic |
| url | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/7969 |
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