Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells
<p>Abstract</p> <p>Background</p> <p>Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess pr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-02-01
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| Series: | Journal of Biomedical Science |
| Online Access: | http://www.jbiomedsci.com/content/16/1/16 |
| _version_ | 1819035250439749632 |
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| author | Sun Lei Pan Wen-Liang Qu Jun-Le Chen Tong-Sheng Lu Ying-Ying Wei Xun-Bin |
| author_facet | Sun Lei Pan Wen-Liang Qu Jun-Le Chen Tong-Sheng Lu Ying-Ying Wei Xun-Bin |
| author_sort | Sun Lei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood.</p> <p>Methods</p> <p>In this study, cell counting kit (CCK-8) assay was employed to evaluate the survival of DHA-treated ASTC-a-1 cells. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. Collapse of mitochondrial transmembrane potential (ΔΨ<sub>m</sub>) was measured by dynamic detection under a laser scanning confocal microscope and flow cytometry analysis using Rhodamine123. Caspase-3 activities measured with or without Z-VAD-fmk (a broad spectrum caspase inhibitor) pretreatment by FRET techniques, caspase-3 activity measurement, and western blotting analysis.</p> <p>Results</p> <p>Our results indicated that DHA induced apoptotic cell death in a dose- and time-dependent manner, which was accompanied by mitochondrial morphology changes, the loss of ΔΨ<sub>m </sub>and the activation of caspase-3.</p> <p>Conclusion</p> <p>These results show for the first time that DHA can inhibit proliferation and induce apoptosis via caspase-3-dependent mitochondrial death pathway in ASTC-a-1 cells. Our work may provide evidence for further studies of DHA as a possible anticancer drug in the clinical treatment of lung adenocarcinoma.</p> |
| first_indexed | 2024-12-21T07:46:38Z |
| format | Article |
| id | doaj.art-930891baa5004d12ae497ce928bb2224 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-12-21T07:46:38Z |
| publishDate | 2009-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-930891baa5004d12ae497ce928bb22242022-12-21T19:11:11ZengBMCJournal of Biomedical Science1021-77701423-01272009-02-011611610.1186/1423-0127-16-16Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cellsSun LeiPan Wen-LiangQu Jun-LeChen Tong-ShengLu Ying-YingWei Xun-Bin<p>Abstract</p> <p>Background</p> <p>Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood.</p> <p>Methods</p> <p>In this study, cell counting kit (CCK-8) assay was employed to evaluate the survival of DHA-treated ASTC-a-1 cells. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. Collapse of mitochondrial transmembrane potential (ΔΨ<sub>m</sub>) was measured by dynamic detection under a laser scanning confocal microscope and flow cytometry analysis using Rhodamine123. Caspase-3 activities measured with or without Z-VAD-fmk (a broad spectrum caspase inhibitor) pretreatment by FRET techniques, caspase-3 activity measurement, and western blotting analysis.</p> <p>Results</p> <p>Our results indicated that DHA induced apoptotic cell death in a dose- and time-dependent manner, which was accompanied by mitochondrial morphology changes, the loss of ΔΨ<sub>m </sub>and the activation of caspase-3.</p> <p>Conclusion</p> <p>These results show for the first time that DHA can inhibit proliferation and induce apoptosis via caspase-3-dependent mitochondrial death pathway in ASTC-a-1 cells. Our work may provide evidence for further studies of DHA as a possible anticancer drug in the clinical treatment of lung adenocarcinoma.</p>http://www.jbiomedsci.com/content/16/1/16 |
| spellingShingle | Sun Lei Pan Wen-Liang Qu Jun-Le Chen Tong-Sheng Lu Ying-Ying Wei Xun-Bin Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells Journal of Biomedical Science |
| title | Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells |
| title_full | Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells |
| title_fullStr | Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells |
| title_full_unstemmed | Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells |
| title_short | Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells |
| title_sort | dihydroartemisinin dha induces caspase 3 dependent apoptosis in human lung adenocarcinoma astc a 1 cells |
| url | http://www.jbiomedsci.com/content/16/1/16 |
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