OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision
The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6N...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/2/294 |
| _version_ | 1797620821207810048 |
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| author | Jiri Lindovsky Marcela Palkova Viktoriia Symkina Miles Joseph Raishbrook Jan Prochazka Radislav Sedlacek |
| author_facet | Jiri Lindovsky Marcela Palkova Viktoriia Symkina Miles Joseph Raishbrook Jan Prochazka Radislav Sedlacek |
| author_sort | Jiri Lindovsky |
| collection | DOAJ |
| description | The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6NCrl retinal parameters in six age groups between 10 and 100 weeks as well as examples of mild and severe pathologies resulting from knocking out a single protein-coding gene. We also show example data obtained by more detailed analysis or additional methods useful in eye research, for example, the angiography of a superficial and deep vascular complex. We discuss the feasibility of these techniques in conditions demanding a high-throughput approach such as the systemic phenotyping carried out by the International Mouse Phenotyping Consortium. |
| first_indexed | 2024-03-11T08:48:04Z |
| format | Article |
| id | doaj.art-930d656e590b4d7eaa1824d2f3a0cc59 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:48:04Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-930d656e590b4d7eaa1824d2f3a0cc592023-11-16T20:40:59ZengMDPI AGGenes2073-44252023-01-0114229410.3390/genes14020294OCT and ERG Techniques in High-Throughput Phenotyping of Mouse VisionJiri Lindovsky0Marcela Palkova1Viktoriia Symkina2Miles Joseph Raishbrook3Jan Prochazka4Radislav Sedlacek5Czech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicThe purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6NCrl retinal parameters in six age groups between 10 and 100 weeks as well as examples of mild and severe pathologies resulting from knocking out a single protein-coding gene. We also show example data obtained by more detailed analysis or additional methods useful in eye research, for example, the angiography of a superficial and deep vascular complex. We discuss the feasibility of these techniques in conditions demanding a high-throughput approach such as the systemic phenotyping carried out by the International Mouse Phenotyping Consortium.https://www.mdpi.com/2073-4425/14/2/294phenotypingvisionOCTERGIMPC |
| spellingShingle | Jiri Lindovsky Marcela Palkova Viktoriia Symkina Miles Joseph Raishbrook Jan Prochazka Radislav Sedlacek OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision Genes phenotyping vision OCT ERG IMPC |
| title | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
| title_full | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
| title_fullStr | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
| title_full_unstemmed | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
| title_short | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
| title_sort | oct and erg techniques in high throughput phenotyping of mouse vision |
| topic | phenotyping vision OCT ERG IMPC |
| url | https://www.mdpi.com/2073-4425/14/2/294 |
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