Method for ultrastructural fine details of plant cuticles by transmission electron microscopy

This method for plant cuticles was adapted from spores and pollen procedure. It already enabled to prepare ultrathin plant cuticle sections of 60–70 nanometers of thickness, in high numbers as a routine, and allowed statistical measurements as any other macro- and/or micro-features. It provides thro...

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Main Author: Gaëtan Guignard
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121001849
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author Gaëtan Guignard
author_facet Gaëtan Guignard
author_sort Gaëtan Guignard
collection DOAJ
description This method for plant cuticles was adapted from spores and pollen procedure. It already enabled to prepare ultrathin plant cuticle sections of 60–70 nanometers of thickness, in high numbers as a routine, and allowed statistical measurements as any other macro- and/or micro-features. It provides through transmission electron microscope clean and contrasted observations of layers and sublayers of the cuticle, at a magnification over 100,000 times. It has been used efficiently for taxonomy, signature of environment and evolution topics since the end of the nineties (Gaëtan Guignard, 2019), however it is detailed here step by step for the first time, and illustrated. It can serve in the future for many purposes, like other taxonomical comparisons, palaeoreconstructions and evolutionary considerations.Value of the protocol • This method provides ultrathin plant cuticle sections of 60–70 nanometers of thickness • Details of layers and sublayers of the cuticle are easily observed • High numbers of sections allows statistical measurements as a routine
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spelling doaj.art-930ebb746330434db85a2c0462ebfef42022-12-21T19:38:07ZengElsevierMethodsX2215-01612021-01-018101391Method for ultrastructural fine details of plant cuticles by transmission electron microscopyGaëtan Guignard0Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622, Villeurbanne, FranceThis method for plant cuticles was adapted from spores and pollen procedure. It already enabled to prepare ultrathin plant cuticle sections of 60–70 nanometers of thickness, in high numbers as a routine, and allowed statistical measurements as any other macro- and/or micro-features. It provides through transmission electron microscope clean and contrasted observations of layers and sublayers of the cuticle, at a magnification over 100,000 times. It has been used efficiently for taxonomy, signature of environment and evolution topics since the end of the nineties (Gaëtan Guignard, 2019), however it is detailed here step by step for the first time, and illustrated. It can serve in the future for many purposes, like other taxonomical comparisons, palaeoreconstructions and evolutionary considerations.Value of the protocol • This method provides ultrathin plant cuticle sections of 60–70 nanometers of thickness • Details of layers and sublayers of the cuticle are easily observed • High numbers of sections allows statistical measurements as a routinehttp://www.sciencedirect.com/science/article/pii/S2215016121001849Method for ultra-thin sections of plant cuticles
spellingShingle Gaëtan Guignard
Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
MethodsX
Method for ultra-thin sections of plant cuticles
title Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
title_full Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
title_fullStr Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
title_full_unstemmed Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
title_short Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
title_sort method for ultrastructural fine details of plant cuticles by transmission electron microscopy
topic Method for ultra-thin sections of plant cuticles
url http://www.sciencedirect.com/science/article/pii/S2215016121001849
work_keys_str_mv AT gaetanguignard methodforultrastructuralfinedetailsofplantcuticlesbytransmissionelectronmicroscopy