Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress
The sweet potato is very sensitive to low temperature. Our previous study revealed that <i>IbMPK3</i>-overexpressing transgenic sweet potato (M3) plants showed stronger low-temperature stress tolerance than wild-type plants (WT). However, the mechanism of M3 plants in response to low-tem...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/7/1247 |
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| author | Rong Jin Tao Yu Pengyu Guo Ming Liu Jiaquan Pan Peng Zhao Qiangqiang Zhang Xiaoya Zhu Jing Wang Aijun Zhang Qinghe Cao Zhonghou Tang |
| author_facet | Rong Jin Tao Yu Pengyu Guo Ming Liu Jiaquan Pan Peng Zhao Qiangqiang Zhang Xiaoya Zhu Jing Wang Aijun Zhang Qinghe Cao Zhonghou Tang |
| author_sort | Rong Jin |
| collection | DOAJ |
| description | The sweet potato is very sensitive to low temperature. Our previous study revealed that <i>IbMPK3</i>-overexpressing transgenic sweet potato (M3) plants showed stronger low-temperature stress tolerance than wild-type plants (WT). However, the mechanism of M3 plants in response to low-temperature stress is unclear. To further analyze how <i>IbMPK3</i> mediates low-temperature stress in sweet potato, WT and M3 plants were exposed to low-temperature stress for 2 h and 12 h for RNA-seq analysis, whereas normal conditions were used as a control (CK). In total, 3436 and 8718 differentially expressed genes (DEGs) were identified in WT at 2 h (vs. CK) and 12 h (vs. CK) under low-temperature stress, respectively, whereas 1450 and 9291 DEGs were detected in M3 plants, respectively. Many common and unique DEGs were analyzed in WT and M3 plants. DEGs related to low temperature were involved in Ca<sup>2+</sup> signaling, MAPK cascades, the reactive oxygen species (ROS) signaling pathway, hormone transduction pathway, encoding transcription factor families (<i>bHLH</i>, <i>NAC</i>, and <i>WRKY</i>), and downstream stress-related genes. Additionally, more upregulated genes were associated with the MAPK pathway in M3 plants during short-term low-temperature stress (CK vs. 2 h), and more upregulated genes were involved in secondary metabolic synthesis in M3 plants than in the WT during the long-time low-temperature stress treatment (CK vs. 12 h), such as fatty acid biosynthesis and elongation, glutathione metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and zeatin biosynthesis. Moreover, the interaction proteins of IbMPK3 related to photosynthesis, or encoding CaM, NAC, and ribosomal proteins, were identified using yeast two-hybrid (Y2H). This study may provide a valuable resource for elucidating the sweet potato low-temperature stress resistance mechanism, as well as data to support molecular-assisted breeding with the <i>IbMPK3</i> gene. |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:18:30Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-9313521d85884fafbedf98037a0ab39d2023-12-01T22:11:49ZengMDPI AGGenes2073-44252022-07-01137124710.3390/genes13071247Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature StressRong Jin0Tao Yu1Pengyu Guo2Ming Liu3Jiaquan Pan4Peng Zhao5Qiangqiang Zhang6Xiaoya Zhu7Jing Wang8Aijun Zhang9Qinghe Cao10Zhonghou Tang11Xuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaTube Division, Crop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaTube Division, Crop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaXuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, ChinaThe sweet potato is very sensitive to low temperature. Our previous study revealed that <i>IbMPK3</i>-overexpressing transgenic sweet potato (M3) plants showed stronger low-temperature stress tolerance than wild-type plants (WT). However, the mechanism of M3 plants in response to low-temperature stress is unclear. To further analyze how <i>IbMPK3</i> mediates low-temperature stress in sweet potato, WT and M3 plants were exposed to low-temperature stress for 2 h and 12 h for RNA-seq analysis, whereas normal conditions were used as a control (CK). In total, 3436 and 8718 differentially expressed genes (DEGs) were identified in WT at 2 h (vs. CK) and 12 h (vs. CK) under low-temperature stress, respectively, whereas 1450 and 9291 DEGs were detected in M3 plants, respectively. Many common and unique DEGs were analyzed in WT and M3 plants. DEGs related to low temperature were involved in Ca<sup>2+</sup> signaling, MAPK cascades, the reactive oxygen species (ROS) signaling pathway, hormone transduction pathway, encoding transcription factor families (<i>bHLH</i>, <i>NAC</i>, and <i>WRKY</i>), and downstream stress-related genes. Additionally, more upregulated genes were associated with the MAPK pathway in M3 plants during short-term low-temperature stress (CK vs. 2 h), and more upregulated genes were involved in secondary metabolic synthesis in M3 plants than in the WT during the long-time low-temperature stress treatment (CK vs. 12 h), such as fatty acid biosynthesis and elongation, glutathione metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and zeatin biosynthesis. Moreover, the interaction proteins of IbMPK3 related to photosynthesis, or encoding CaM, NAC, and ribosomal proteins, were identified using yeast two-hybrid (Y2H). This study may provide a valuable resource for elucidating the sweet potato low-temperature stress resistance mechanism, as well as data to support molecular-assisted breeding with the <i>IbMPK3</i> gene.https://www.mdpi.com/2073-4425/13/7/1247<i>IbMPK3</i>-overexpressing transgenic sweet potato plantslow-temperature stresstranscriptome analysisprotein interaction |
| spellingShingle | Rong Jin Tao Yu Pengyu Guo Ming Liu Jiaquan Pan Peng Zhao Qiangqiang Zhang Xiaoya Zhu Jing Wang Aijun Zhang Qinghe Cao Zhonghou Tang Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress Genes <i>IbMPK3</i>-overexpressing transgenic sweet potato plants low-temperature stress transcriptome analysis protein interaction |
| title | Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress |
| title_full | Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress |
| title_fullStr | Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress |
| title_full_unstemmed | Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress |
| title_short | Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of <i>IbMPK3</i>-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress |
| title_sort | comparative transcriptome and interaction protein analysis reveals the mechanism of i ibmpk3 i overexpressing transgenic sweet potato response to low temperature stress |
| topic | <i>IbMPK3</i>-overexpressing transgenic sweet potato plants low-temperature stress transcriptome analysis protein interaction |
| url | https://www.mdpi.com/2073-4425/13/7/1247 |
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