Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners
Background Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study w...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-09-01
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| Series: | Journal of the International Society of Sports Nutrition |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1186/s12970-020-00376-3 |
| _version_ | 1827817774199603200 |
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| author | Mette Hansen Mikkel Oxfeldt Anne E. Larsen Lise S. Thomsen Torben Rokkedal-Lausch Britt Christensen Nikolaj Rittig Frank V. De Paoli Jens Bangsbo Niels Ørtenblad Klavs Madsen |
| author_facet | Mette Hansen Mikkel Oxfeldt Anne E. Larsen Lise S. Thomsen Torben Rokkedal-Lausch Britt Christensen Nikolaj Rittig Frank V. De Paoli Jens Bangsbo Niels Ørtenblad Klavs Madsen |
| author_sort | Mette Hansen |
| collection | DOAJ |
| description | Background Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners. Methods Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg− 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg− 1) before and protein-carbohydrate beverage (0.3 g protein kg− 1 and 1 g carbohydrate kg− 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention. Results Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07–0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups. Conclusion Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance. Trial registration clinicaltrials.gov, NCT03561337. Registered 6 June 2018 – Retrospectively registered. |
| first_indexed | 2024-03-12T00:38:25Z |
| format | Article |
| id | doaj.art-93176b8523e44964a157705fe639138a |
| institution | Directory Open Access Journal |
| issn | 1550-2783 |
| language | English |
| last_indexed | 2024-03-12T00:38:25Z |
| publishDate | 2020-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of the International Society of Sports Nutrition |
| spelling | doaj.art-93176b8523e44964a157705fe639138a2023-09-15T09:19:31ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832020-09-0117110.1186/s12970-020-00376-312130185Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runnersMette Hansen0Mikkel Oxfeldt1Anne E. Larsen2Lise S. Thomsen3Torben Rokkedal-Lausch4Britt Christensen5Nikolaj Rittig6Frank V. De Paoli7Jens Bangsbo8Niels Ørtenblad9Klavs Madsen10Section for Sport Sciences, Department of Public HealthSection for Sport Sciences, Department of Public HealthSection for Sport Sciences, Department of Public HealthDepartment of Sport Science and Clinical BiomechanicsDepartment of Health Science and TechnologyDepartment of Endocrinology and Internal MedicineDepartment of Endocrinology and Internal MedicineDepartment of BiomedicineDepartment of Nutrition, Exercise and SportsDepartment of Sport Science and Clinical BiomechanicsSection for Sport Sciences, Department of Public HealthBackground Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners. Methods Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg− 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg− 1) before and protein-carbohydrate beverage (0.3 g protein kg− 1 and 1 g carbohydrate kg− 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention. Results Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07–0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups. Conclusion Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance. Trial registration clinicaltrials.gov, NCT03561337. Registered 6 June 2018 – Retrospectively registered.http://dx.doi.org/10.1186/s12970-020-00376-3sports nutritionprotein hydrolysateperformanceenzyme activityskeletal musclemitochondriaendurance sport |
| spellingShingle | Mette Hansen Mikkel Oxfeldt Anne E. Larsen Lise S. Thomsen Torben Rokkedal-Lausch Britt Christensen Nikolaj Rittig Frank V. De Paoli Jens Bangsbo Niels Ørtenblad Klavs Madsen Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners Journal of the International Society of Sports Nutrition sports nutrition protein hydrolysate performance enzyme activity skeletal muscle mitochondria endurance sport |
| title | Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| title_full | Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| title_fullStr | Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| title_full_unstemmed | Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| title_short | Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| title_sort | supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners |
| topic | sports nutrition protein hydrolysate performance enzyme activity skeletal muscle mitochondria endurance sport |
| url | http://dx.doi.org/10.1186/s12970-020-00376-3 |
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