Milk fat miRNome changes in response to LPS challenge in Holstein cows
Abstract Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-023-01231-4 |
| _version_ | 1827633437821894656 |
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| author | Christine Leroux Matteo Cuccato Karol Pawłowski Francesca Tiziana Cannizzo Paola Sacchi José A. A. Pires Yannick Faulconnier |
| author_facet | Christine Leroux Matteo Cuccato Karol Pawłowski Francesca Tiziana Cannizzo Paola Sacchi José A. A. Pires Yannick Faulconnier |
| author_sort | Christine Leroux |
| collection | DOAJ |
| description | Abstract Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (miRNome) of milk fat, using microarray analyses in cows. Cows were fed a lactation diet at ad libitum intake (n = 6). At 27 ± 3 days in milk, cows were injected with 50 µg of LPS Escherichia coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS−) and 6.5 h after LPS challenge (LPS +) from the same cows. Microarray analysis was performed using customized 8 × 60 K ruminant miRNA microarrays to compare LPS− to LPS + miRNome. In silico functional analyses were performed using OmicsNet and Mienturnet software. MiRNome comparison between LPS− and LPS + identified 37 differentially abundant miRNAs (q-value ≤ 0.05). The predicted target genes of the 37 differentially abundant miRNAs are mostly involved in cell life including apoptosis, cell cycle, proliferation and differentiation and in gene expression processes. MiRNome analyses suggest that miRNAs profile is related to the inflammation response of the mammary gland. In conclusion, we demonstrated that milk fat might be an easy and rapid source of miRNAs that are potential indicators of early mastitis in cows. |
| first_indexed | 2024-03-09T14:59:12Z |
| format | Article |
| id | doaj.art-9318b4cc87654378b79c2aa6ce6723e4 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-09T14:59:12Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-9318b4cc87654378b79c2aa6ce6723e42023-11-26T13:59:53ZengBMCVeterinary Research1297-97162023-11-0154111010.1186/s13567-023-01231-4Milk fat miRNome changes in response to LPS challenge in Holstein cowsChristine Leroux0Matteo Cuccato1Karol Pawłowski2Francesca Tiziana Cannizzo3Paola Sacchi4José A. A. Pires5Yannick Faulconnier6INRAE, Université Clermont AuvergneINRAE, Université Clermont AuvergneINRAE, Université Clermont AuvergneDipartimento di Scienze Veterinarie, Università degli Studi di TorinoDipartimento di Scienze Veterinarie, Università degli Studi di TorinoINRAE, Université Clermont AuvergneINRAE, Université Clermont AuvergneAbstract Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (miRNome) of milk fat, using microarray analyses in cows. Cows were fed a lactation diet at ad libitum intake (n = 6). At 27 ± 3 days in milk, cows were injected with 50 µg of LPS Escherichia coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS−) and 6.5 h after LPS challenge (LPS +) from the same cows. Microarray analysis was performed using customized 8 × 60 K ruminant miRNA microarrays to compare LPS− to LPS + miRNome. In silico functional analyses were performed using OmicsNet and Mienturnet software. MiRNome comparison between LPS− and LPS + identified 37 differentially abundant miRNAs (q-value ≤ 0.05). The predicted target genes of the 37 differentially abundant miRNAs are mostly involved in cell life including apoptosis, cell cycle, proliferation and differentiation and in gene expression processes. MiRNome analyses suggest that miRNAs profile is related to the inflammation response of the mammary gland. In conclusion, we demonstrated that milk fat might be an easy and rapid source of miRNAs that are potential indicators of early mastitis in cows.https://doi.org/10.1186/s13567-023-01231-4miRNomesmilk fatdairy cowinflammationLPS |
| spellingShingle | Christine Leroux Matteo Cuccato Karol Pawłowski Francesca Tiziana Cannizzo Paola Sacchi José A. A. Pires Yannick Faulconnier Milk fat miRNome changes in response to LPS challenge in Holstein cows Veterinary Research miRNomes milk fat dairy cow inflammation LPS |
| title | Milk fat miRNome changes in response to LPS challenge in Holstein cows |
| title_full | Milk fat miRNome changes in response to LPS challenge in Holstein cows |
| title_fullStr | Milk fat miRNome changes in response to LPS challenge in Holstein cows |
| title_full_unstemmed | Milk fat miRNome changes in response to LPS challenge in Holstein cows |
| title_short | Milk fat miRNome changes in response to LPS challenge in Holstein cows |
| title_sort | milk fat mirnome changes in response to lps challenge in holstein cows |
| topic | miRNomes milk fat dairy cow inflammation LPS |
| url | https://doi.org/10.1186/s13567-023-01231-4 |
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